Cargando…

Molecular evidence of the avocado defense response to Fusarium kuroshium infection: a deep transcriptome analysis using RNA-Seq

Fusarium kuroshium is a novel member of the Ambrosia Fusarium Clade (AFC) that has been recognized as one of the symbionts of the invasive Kuroshio shot hole borer, an Asian ambrosia beetle. This complex is considered the causal agent of Fusarium dieback, a disease that has severely threatened natur...

Descripción completa

Detalles Bibliográficos
Autores principales: Pérez-Torres, Claudia-Anahí, Ibarra-Laclette, Enrique, Hernández-Domínguez, Eric-Edmundo, Rodríguez-Haas, Benjamín, Pérez-Lira, Alan-Josué, Villafán, Emanuel, Alonso-Sánchez, Alexandro, García-Ávila, Clemente de Jesús, Ramírez-Pool, José-Abrahán, Sánchez-Rangel, Diana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052963/
https://www.ncbi.nlm.nih.gov/pubmed/33954045
http://dx.doi.org/10.7717/peerj.11215
_version_ 1783680027021279232
author Pérez-Torres, Claudia-Anahí
Ibarra-Laclette, Enrique
Hernández-Domínguez, Eric-Edmundo
Rodríguez-Haas, Benjamín
Pérez-Lira, Alan-Josué
Villafán, Emanuel
Alonso-Sánchez, Alexandro
García-Ávila, Clemente de Jesús
Ramírez-Pool, José-Abrahán
Sánchez-Rangel, Diana
author_facet Pérez-Torres, Claudia-Anahí
Ibarra-Laclette, Enrique
Hernández-Domínguez, Eric-Edmundo
Rodríguez-Haas, Benjamín
Pérez-Lira, Alan-Josué
Villafán, Emanuel
Alonso-Sánchez, Alexandro
García-Ávila, Clemente de Jesús
Ramírez-Pool, José-Abrahán
Sánchez-Rangel, Diana
author_sort Pérez-Torres, Claudia-Anahí
collection PubMed
description Fusarium kuroshium is a novel member of the Ambrosia Fusarium Clade (AFC) that has been recognized as one of the symbionts of the invasive Kuroshio shot hole borer, an Asian ambrosia beetle. This complex is considered the causal agent of Fusarium dieback, a disease that has severely threatened natural forests, landscape trees, and avocado orchards in the last 8 years. Despite the interest in this species, the molecular responses of both the host and F. kuroshium during the infection process and disease establishment remain unknown. In this work, we established an in vitro pathosystem using Hass avocado stems inoculated with F. kuroshium to investigate differential gene expression at 1, 4, 7 and 14 days post-inoculation. RNA-seq technology allowed us to obtain data from both the plant and the fungus, and the sequences obtained from both organisms were analyzed independently. The pathosystem established was able to mimic Fusarium dieback symptoms, such as carbohydrate exudation, necrosis, and vascular tissue discoloration. The results provide interesting evidence regarding the genes that may play roles in the avocado defense response to Fusarium dieback disease. The avocado data set comprised a coding sequence collection of 51,379 UniGenes, from which 2,403 (4.67%) were identified as differentially expressed. The global expression analysis showed that F. kuroshium responsive UniGenes can be clustered into six groups according to their expression profiles. The biologically relevant functional categories that were identified included photosynthesis as well as responses to stress, hormones, abscisic acid, and water deprivation. Additionally, processes such as oxidation-reduction, organization and biogenesis of the cell wall and polysaccharide metabolism were detected. Moreover, we identified orthologues of nucleotide-binding leucine-rich receptors, and their possible action mode was analyzed. In F. kuroshium, we identified 57 differentially expressed genes. Interestingly, the alcohol metabolic process biological category had the highest number of upregulated genes, and the enzyme group in this category may play an important role in the mechanisms of secondary metabolite detoxification. Hydrolytic enzymes, such as endoglucanases and a pectate lyase, were also identified, as well as some proteases. In conclusion, our research was conducted mainly to explain how the vascular tissue of a recognized host of the ambrosia complex responds during F. kuroshium infection since Fusarium dieback is an ambrosia beetle-vectored disease and many variables facilitate its establishment.
format Online
Article
Text
id pubmed-8052963
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher PeerJ Inc.
record_format MEDLINE/PubMed
spelling pubmed-80529632021-05-04 Molecular evidence of the avocado defense response to Fusarium kuroshium infection: a deep transcriptome analysis using RNA-Seq Pérez-Torres, Claudia-Anahí Ibarra-Laclette, Enrique Hernández-Domínguez, Eric-Edmundo Rodríguez-Haas, Benjamín Pérez-Lira, Alan-Josué Villafán, Emanuel Alonso-Sánchez, Alexandro García-Ávila, Clemente de Jesús Ramírez-Pool, José-Abrahán Sánchez-Rangel, Diana PeerJ Agricultural Science Fusarium kuroshium is a novel member of the Ambrosia Fusarium Clade (AFC) that has been recognized as one of the symbionts of the invasive Kuroshio shot hole borer, an Asian ambrosia beetle. This complex is considered the causal agent of Fusarium dieback, a disease that has severely threatened natural forests, landscape trees, and avocado orchards in the last 8 years. Despite the interest in this species, the molecular responses of both the host and F. kuroshium during the infection process and disease establishment remain unknown. In this work, we established an in vitro pathosystem using Hass avocado stems inoculated with F. kuroshium to investigate differential gene expression at 1, 4, 7 and 14 days post-inoculation. RNA-seq technology allowed us to obtain data from both the plant and the fungus, and the sequences obtained from both organisms were analyzed independently. The pathosystem established was able to mimic Fusarium dieback symptoms, such as carbohydrate exudation, necrosis, and vascular tissue discoloration. The results provide interesting evidence regarding the genes that may play roles in the avocado defense response to Fusarium dieback disease. The avocado data set comprised a coding sequence collection of 51,379 UniGenes, from which 2,403 (4.67%) were identified as differentially expressed. The global expression analysis showed that F. kuroshium responsive UniGenes can be clustered into six groups according to their expression profiles. The biologically relevant functional categories that were identified included photosynthesis as well as responses to stress, hormones, abscisic acid, and water deprivation. Additionally, processes such as oxidation-reduction, organization and biogenesis of the cell wall and polysaccharide metabolism were detected. Moreover, we identified orthologues of nucleotide-binding leucine-rich receptors, and their possible action mode was analyzed. In F. kuroshium, we identified 57 differentially expressed genes. Interestingly, the alcohol metabolic process biological category had the highest number of upregulated genes, and the enzyme group in this category may play an important role in the mechanisms of secondary metabolite detoxification. Hydrolytic enzymes, such as endoglucanases and a pectate lyase, were also identified, as well as some proteases. In conclusion, our research was conducted mainly to explain how the vascular tissue of a recognized host of the ambrosia complex responds during F. kuroshium infection since Fusarium dieback is an ambrosia beetle-vectored disease and many variables facilitate its establishment. PeerJ Inc. 2021-04-14 /pmc/articles/PMC8052963/ /pubmed/33954045 http://dx.doi.org/10.7717/peerj.11215 Text en © 2021 Pérez-Torres et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Pérez-Torres, Claudia-Anahí
Ibarra-Laclette, Enrique
Hernández-Domínguez, Eric-Edmundo
Rodríguez-Haas, Benjamín
Pérez-Lira, Alan-Josué
Villafán, Emanuel
Alonso-Sánchez, Alexandro
García-Ávila, Clemente de Jesús
Ramírez-Pool, José-Abrahán
Sánchez-Rangel, Diana
Molecular evidence of the avocado defense response to Fusarium kuroshium infection: a deep transcriptome analysis using RNA-Seq
title Molecular evidence of the avocado defense response to Fusarium kuroshium infection: a deep transcriptome analysis using RNA-Seq
title_full Molecular evidence of the avocado defense response to Fusarium kuroshium infection: a deep transcriptome analysis using RNA-Seq
title_fullStr Molecular evidence of the avocado defense response to Fusarium kuroshium infection: a deep transcriptome analysis using RNA-Seq
title_full_unstemmed Molecular evidence of the avocado defense response to Fusarium kuroshium infection: a deep transcriptome analysis using RNA-Seq
title_short Molecular evidence of the avocado defense response to Fusarium kuroshium infection: a deep transcriptome analysis using RNA-Seq
title_sort molecular evidence of the avocado defense response to fusarium kuroshium infection: a deep transcriptome analysis using rna-seq
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052963/
https://www.ncbi.nlm.nih.gov/pubmed/33954045
http://dx.doi.org/10.7717/peerj.11215
work_keys_str_mv AT pereztorresclaudiaanahi molecularevidenceoftheavocadodefenseresponsetofusariumkuroshiuminfectionadeeptranscriptomeanalysisusingrnaseq
AT ibarralacletteenrique molecularevidenceoftheavocadodefenseresponsetofusariumkuroshiuminfectionadeeptranscriptomeanalysisusingrnaseq
AT hernandezdominguezericedmundo molecularevidenceoftheavocadodefenseresponsetofusariumkuroshiuminfectionadeeptranscriptomeanalysisusingrnaseq
AT rodriguezhaasbenjamin molecularevidenceoftheavocadodefenseresponsetofusariumkuroshiuminfectionadeeptranscriptomeanalysisusingrnaseq
AT perezliraalanjosue molecularevidenceoftheavocadodefenseresponsetofusariumkuroshiuminfectionadeeptranscriptomeanalysisusingrnaseq
AT villafanemanuel molecularevidenceoftheavocadodefenseresponsetofusariumkuroshiuminfectionadeeptranscriptomeanalysisusingrnaseq
AT alonsosanchezalexandro molecularevidenceoftheavocadodefenseresponsetofusariumkuroshiuminfectionadeeptranscriptomeanalysisusingrnaseq
AT garciaavilaclementedejesus molecularevidenceoftheavocadodefenseresponsetofusariumkuroshiuminfectionadeeptranscriptomeanalysisusingrnaseq
AT ramirezpooljoseabrahan molecularevidenceoftheavocadodefenseresponsetofusariumkuroshiuminfectionadeeptranscriptomeanalysisusingrnaseq
AT sanchezrangeldiana molecularevidenceoftheavocadodefenseresponsetofusariumkuroshiuminfectionadeeptranscriptomeanalysisusingrnaseq