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Molecular Species Identification of Six Forensically Important Iranian Flesh Flies (Diptera)

BACKGROUND: Flesh flies (Diptera: Sarcophagidae) are considered as myiasis agents and important evidences in forensic investigations. However, their use has been restricted because, at all larval stages and female adults, morphological species identification is difficult or very challenging. This st...

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Autores principales: Talebzadeh, Fahimeh, Oshaghi, Mohammad Ali, Akbarzadeh, Kamran, Panahi-Moghadam, Somayeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053068/
https://www.ncbi.nlm.nih.gov/pubmed/33954215
http://dx.doi.org/10.18502/jad.v14i4.5279
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author Talebzadeh, Fahimeh
Oshaghi, Mohammad Ali
Akbarzadeh, Kamran
Panahi-Moghadam, Somayeh
author_facet Talebzadeh, Fahimeh
Oshaghi, Mohammad Ali
Akbarzadeh, Kamran
Panahi-Moghadam, Somayeh
author_sort Talebzadeh, Fahimeh
collection PubMed
description BACKGROUND: Flesh flies (Diptera: Sarcophagidae) are considered as myiasis agents and important evidences in forensic investigations. However, their use has been restricted because, at all larval stages and female adults, morphological species identification is difficult or very challenging. This study investigated to test utility of mitochondrial cytochrome oxidase subunit I (mt-COI) sequences for differentiation of six forensically important Iranian flesh flies namely, Sarcophaga crassipalpis, S. flagellifera, S. hirtipes, S. aegyptica, S. africa and S. argyrostoma. METHODS: Male specimens were morphologically identified to species level and then the genomic DNA of the flies were extracted and subjected to polymerase chain reaction (PCR) against mt-COI gene. The PCR products were sequenced and the obtained sequences were analyzed for the species specific restriction fragment length polymorphisms (RFLPs). RESULTS: Rate of genetic variation between species was 6–10% which was enough to find restriction enzymes (RE) that were able to produce species-specific RFLP profiles. Combinations of three REs: BsrFI, RsaI and HinfI, provided diagnostic bands for identification of the six Sarcophaga species. CONCLUSION: The results of this study showed that molecular markers such as RFLPs enhancing the use of evidence from flesh flies in forensic investigation. However, lack proper restriction sites in the COI region inhibited introduction of a single restriction enzyme for easy species identification. It is recommended to apply larger part of DNA such as combination of COI and COII genes to provide better RFLP markers for species identification of flesh flies.
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spelling pubmed-80530682021-05-04 Molecular Species Identification of Six Forensically Important Iranian Flesh Flies (Diptera) Talebzadeh, Fahimeh Oshaghi, Mohammad Ali Akbarzadeh, Kamran Panahi-Moghadam, Somayeh J Arthropod Borne Dis Original Article BACKGROUND: Flesh flies (Diptera: Sarcophagidae) are considered as myiasis agents and important evidences in forensic investigations. However, their use has been restricted because, at all larval stages and female adults, morphological species identification is difficult or very challenging. This study investigated to test utility of mitochondrial cytochrome oxidase subunit I (mt-COI) sequences for differentiation of six forensically important Iranian flesh flies namely, Sarcophaga crassipalpis, S. flagellifera, S. hirtipes, S. aegyptica, S. africa and S. argyrostoma. METHODS: Male specimens were morphologically identified to species level and then the genomic DNA of the flies were extracted and subjected to polymerase chain reaction (PCR) against mt-COI gene. The PCR products were sequenced and the obtained sequences were analyzed for the species specific restriction fragment length polymorphisms (RFLPs). RESULTS: Rate of genetic variation between species was 6–10% which was enough to find restriction enzymes (RE) that were able to produce species-specific RFLP profiles. Combinations of three REs: BsrFI, RsaI and HinfI, provided diagnostic bands for identification of the six Sarcophaga species. CONCLUSION: The results of this study showed that molecular markers such as RFLPs enhancing the use of evidence from flesh flies in forensic investigation. However, lack proper restriction sites in the COI region inhibited introduction of a single restriction enzyme for easy species identification. It is recommended to apply larger part of DNA such as combination of COI and COII genes to provide better RFLP markers for species identification of flesh flies. Tehran University of Medical Sciences 2020-12-31 /pmc/articles/PMC8053068/ /pubmed/33954215 http://dx.doi.org/10.18502/jad.v14i4.5279 Text en Copyright © 2020 The Authors. Published by Tehran University of Medical Sciences https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International license (https://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited.
spellingShingle Original Article
Talebzadeh, Fahimeh
Oshaghi, Mohammad Ali
Akbarzadeh, Kamran
Panahi-Moghadam, Somayeh
Molecular Species Identification of Six Forensically Important Iranian Flesh Flies (Diptera)
title Molecular Species Identification of Six Forensically Important Iranian Flesh Flies (Diptera)
title_full Molecular Species Identification of Six Forensically Important Iranian Flesh Flies (Diptera)
title_fullStr Molecular Species Identification of Six Forensically Important Iranian Flesh Flies (Diptera)
title_full_unstemmed Molecular Species Identification of Six Forensically Important Iranian Flesh Flies (Diptera)
title_short Molecular Species Identification of Six Forensically Important Iranian Flesh Flies (Diptera)
title_sort molecular species identification of six forensically important iranian flesh flies (diptera)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053068/
https://www.ncbi.nlm.nih.gov/pubmed/33954215
http://dx.doi.org/10.18502/jad.v14i4.5279
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