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Expanding of ST11 Carbapenemase-Producing Klebsiella pneumoniae Subclones in a Chinese Hospital, Shenzhen, China

BACKGROUND: ST11 is the most prevalent sequence type of clinical Klebsiella pneumoniae in China. METHODS: We investigated the characteristics of the ST11 subclones using core genome multi-locus sequence typing (cgMLST). Ninety-three carbapenemase-producing K. pneumoniae isolates were collected at Sh...

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Autores principales: Zhang, Min, Li, Jia, Lu, Yuemei, Wu, Weiyuan, Wu, Jinsong, Xu, Yanji, Zhong, Yuxin, Liu, Shiyi, Lin, Chuchu, Xu, Shuye, Chen, Dandan, Gao, Xuefeng, Luo, Yutian, Chen, Huaisheng, Liu, Xueyan, Huang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053513/
https://www.ncbi.nlm.nih.gov/pubmed/33880046
http://dx.doi.org/10.2147/IDR.S299478
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author Zhang, Min
Li, Jia
Lu, Yuemei
Wu, Weiyuan
Wu, Jinsong
Xu, Yanji
Zhong, Yuxin
Liu, Shiyi
Lin, Chuchu
Xu, Shuye
Chen, Dandan
Gao, Xuefeng
Luo, Yutian
Chen, Huaisheng
Liu, Xueyan
Huang, Wei
author_facet Zhang, Min
Li, Jia
Lu, Yuemei
Wu, Weiyuan
Wu, Jinsong
Xu, Yanji
Zhong, Yuxin
Liu, Shiyi
Lin, Chuchu
Xu, Shuye
Chen, Dandan
Gao, Xuefeng
Luo, Yutian
Chen, Huaisheng
Liu, Xueyan
Huang, Wei
author_sort Zhang, Min
collection PubMed
description BACKGROUND: ST11 is the most prevalent sequence type of clinical Klebsiella pneumoniae in China. METHODS: We investigated the characteristics of the ST11 subclones using core genome multi-locus sequence typing (cgMLST). Ninety-three carbapenemase-producing K. pneumoniae isolates were collected at Shenzhen People’s Hospital. Then, whole-genome sequencing and cgMLST were used to discriminate apparent subclones within the ST11 group. RESULTS: We analyzed the prevalence and genetic relationships of these subclones. ST11 and K. pneumoniae carbapenemase (KPC-2) were the predominant genotype and carbapenemase, respectively, in the clinical carbapenemase-producing K. pneumoniae strains. cgMLST scheme genotyping divided the ST11 group into two clades across seven complex types (CTs). CT1313 was the most prevalent subclone. The deletion of galF and a high frequency of SNPs in genes associated with the stress- and SOS-responses were found in CT1291 and CT2405 over time, respectively. CONCLUSION: Our results indicated that the subclones of the ST11 group had different patterns of prevalence. Highly discriminatory genotyping techniques, such as cgMLST scheme, should be used in further molecular epidemiology investigations.
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spelling pubmed-80535132021-04-19 Expanding of ST11 Carbapenemase-Producing Klebsiella pneumoniae Subclones in a Chinese Hospital, Shenzhen, China Zhang, Min Li, Jia Lu, Yuemei Wu, Weiyuan Wu, Jinsong Xu, Yanji Zhong, Yuxin Liu, Shiyi Lin, Chuchu Xu, Shuye Chen, Dandan Gao, Xuefeng Luo, Yutian Chen, Huaisheng Liu, Xueyan Huang, Wei Infect Drug Resist Original Research BACKGROUND: ST11 is the most prevalent sequence type of clinical Klebsiella pneumoniae in China. METHODS: We investigated the characteristics of the ST11 subclones using core genome multi-locus sequence typing (cgMLST). Ninety-three carbapenemase-producing K. pneumoniae isolates were collected at Shenzhen People’s Hospital. Then, whole-genome sequencing and cgMLST were used to discriminate apparent subclones within the ST11 group. RESULTS: We analyzed the prevalence and genetic relationships of these subclones. ST11 and K. pneumoniae carbapenemase (KPC-2) were the predominant genotype and carbapenemase, respectively, in the clinical carbapenemase-producing K. pneumoniae strains. cgMLST scheme genotyping divided the ST11 group into two clades across seven complex types (CTs). CT1313 was the most prevalent subclone. The deletion of galF and a high frequency of SNPs in genes associated with the stress- and SOS-responses were found in CT1291 and CT2405 over time, respectively. CONCLUSION: Our results indicated that the subclones of the ST11 group had different patterns of prevalence. Highly discriminatory genotyping techniques, such as cgMLST scheme, should be used in further molecular epidemiology investigations. Dove 2021-04-13 /pmc/articles/PMC8053513/ /pubmed/33880046 http://dx.doi.org/10.2147/IDR.S299478 Text en © 2021 Zhang et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Zhang, Min
Li, Jia
Lu, Yuemei
Wu, Weiyuan
Wu, Jinsong
Xu, Yanji
Zhong, Yuxin
Liu, Shiyi
Lin, Chuchu
Xu, Shuye
Chen, Dandan
Gao, Xuefeng
Luo, Yutian
Chen, Huaisheng
Liu, Xueyan
Huang, Wei
Expanding of ST11 Carbapenemase-Producing Klebsiella pneumoniae Subclones in a Chinese Hospital, Shenzhen, China
title Expanding of ST11 Carbapenemase-Producing Klebsiella pneumoniae Subclones in a Chinese Hospital, Shenzhen, China
title_full Expanding of ST11 Carbapenemase-Producing Klebsiella pneumoniae Subclones in a Chinese Hospital, Shenzhen, China
title_fullStr Expanding of ST11 Carbapenemase-Producing Klebsiella pneumoniae Subclones in a Chinese Hospital, Shenzhen, China
title_full_unstemmed Expanding of ST11 Carbapenemase-Producing Klebsiella pneumoniae Subclones in a Chinese Hospital, Shenzhen, China
title_short Expanding of ST11 Carbapenemase-Producing Klebsiella pneumoniae Subclones in a Chinese Hospital, Shenzhen, China
title_sort expanding of st11 carbapenemase-producing klebsiella pneumoniae subclones in a chinese hospital, shenzhen, china
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053513/
https://www.ncbi.nlm.nih.gov/pubmed/33880046
http://dx.doi.org/10.2147/IDR.S299478
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