In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture

In vitro differentiation of human pluripotent stem cells (hPSCs) offers a genetically tractable system to examine the physiology and pathology of human tissue development and differentiation. We have used this approach to model the earliest stages of human B lineage development and characterize pote...

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Autores principales: Richardson, Simon E., Ghazanfari, Roshanak, Chhetri, Jyoti, Enver, Tariq, Böiers, Charlotta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053808/
https://www.ncbi.nlm.nih.gov/pubmed/33899010
http://dx.doi.org/10.1016/j.xpro.2021.100420
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author Richardson, Simon E.
Ghazanfari, Roshanak
Chhetri, Jyoti
Enver, Tariq
Böiers, Charlotta
author_facet Richardson, Simon E.
Ghazanfari, Roshanak
Chhetri, Jyoti
Enver, Tariq
Böiers, Charlotta
author_sort Richardson, Simon E.
collection PubMed
description In vitro differentiation of human pluripotent stem cells (hPSCs) offers a genetically tractable system to examine the physiology and pathology of human tissue development and differentiation. We have used this approach to model the earliest stages of human B lineage development and characterize potential target cells for the in utero initiation of childhood B acute lymphoblastic leukemia. Herein, we detail critical aspects of the protocol including reagent validation, controls, and examples of surface markers used for analysis and cell sorting. For complete details on the use and execution of this protocol, please refer to Boiers et al. (2018).
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spelling pubmed-80538082021-04-22 In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture Richardson, Simon E. Ghazanfari, Roshanak Chhetri, Jyoti Enver, Tariq Böiers, Charlotta STAR Protoc Protocol In vitro differentiation of human pluripotent stem cells (hPSCs) offers a genetically tractable system to examine the physiology and pathology of human tissue development and differentiation. We have used this approach to model the earliest stages of human B lineage development and characterize potential target cells for the in utero initiation of childhood B acute lymphoblastic leukemia. Herein, we detail critical aspects of the protocol including reagent validation, controls, and examples of surface markers used for analysis and cell sorting. For complete details on the use and execution of this protocol, please refer to Boiers et al. (2018). Elsevier 2021-03-31 /pmc/articles/PMC8053808/ /pubmed/33899010 http://dx.doi.org/10.1016/j.xpro.2021.100420 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Richardson, Simon E.
Ghazanfari, Roshanak
Chhetri, Jyoti
Enver, Tariq
Böiers, Charlotta
In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture
title In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture
title_full In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture
title_fullStr In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture
title_full_unstemmed In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture
title_short In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture
title_sort in vitro differentiation of human pluripotent stem cells into the b lineage using op9-ms5 co-culture
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053808/
https://www.ncbi.nlm.nih.gov/pubmed/33899010
http://dx.doi.org/10.1016/j.xpro.2021.100420
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