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New pathway ameliorating ulcerative colitis: focus on Roseburia intestinalis and the gut–brain axis

BACKGROUND: The community of gut microbes is a key factor controlling the intestinal barrier that communicates with the nervous system through the gut–brain axis. Based on our clinical data showing that populations of Roseburia intestinalis are dramatically decreased in the gut of patients with ulce...

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Autores principales: Xu, Fenghua, Cheng, Yi, Ruan, Guangcong, Fan, Liqin, Tian, Yuting, Xiao, Zhifeng, Chen, Dongfeng, Wei, Yanling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053823/
https://www.ncbi.nlm.nih.gov/pubmed/33948112
http://dx.doi.org/10.1177/17562848211004469
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author Xu, Fenghua
Cheng, Yi
Ruan, Guangcong
Fan, Liqin
Tian, Yuting
Xiao, Zhifeng
Chen, Dongfeng
Wei, Yanling
author_facet Xu, Fenghua
Cheng, Yi
Ruan, Guangcong
Fan, Liqin
Tian, Yuting
Xiao, Zhifeng
Chen, Dongfeng
Wei, Yanling
author_sort Xu, Fenghua
collection PubMed
description BACKGROUND: The community of gut microbes is a key factor controlling the intestinal barrier that communicates with the nervous system through the gut–brain axis. Based on our clinical data showing that populations of Roseburia intestinalis are dramatically decreased in the gut of patients with ulcerative colitis, we studied the efficacy of a strain belonging to this species in the context of colitis and stress using animal models. METHODS: Dextran sulfate sodium was used to induce colitis in rats, which then underwent an enema with R. intestinalis as a treatment. The disease activity index, fecal changes and body weight of rats were recorded to evaluate colitis, while histological and immunohistochemical analyses were carried out to examine colon function, and 16S rRNA sequencing was performed to evaluate the gut microbiota change. Behavioral assays and immunohistochemical staining of brain were performed to assess the effect of R. intestinalis on the gut–brain axis. RESULTS: Colitis-related symptoms in rats were significantly relieved after R. intestinalis enema, and the stool traits and colon length of rats were significantly recovered after treatment. The gut epithelial integrity and intestinal barrier were restored in treated rats, as evidenced by the higher expression of Zo-1 in colon tissues, accompanied by the restoration of gut microbiota. Meanwhile, depressive-like behaviors of rats were reduced after treatment, and laboratory experiments on neuronal cells also showed that IL-6, IL-7 and 5-HT were downregulated by R. intestinalis treatment in both serum and brain tissue, while Iba-1 expression was reduced in treated rats. CONCLUSIONS: The administration of R. intestinalis contributes to restoration of the gut microbiota, promoting colon repair and the recovery of gastrointestinal function. These alterations are accompanied by the relief of depressive-like behaviors through a process modulated by the neuronal network and the regulation of inflammation by the gut–brain axis.
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spelling pubmed-80538232021-05-03 New pathway ameliorating ulcerative colitis: focus on Roseburia intestinalis and the gut–brain axis Xu, Fenghua Cheng, Yi Ruan, Guangcong Fan, Liqin Tian, Yuting Xiao, Zhifeng Chen, Dongfeng Wei, Yanling Therap Adv Gastroenterol Original Research BACKGROUND: The community of gut microbes is a key factor controlling the intestinal barrier that communicates with the nervous system through the gut–brain axis. Based on our clinical data showing that populations of Roseburia intestinalis are dramatically decreased in the gut of patients with ulcerative colitis, we studied the efficacy of a strain belonging to this species in the context of colitis and stress using animal models. METHODS: Dextran sulfate sodium was used to induce colitis in rats, which then underwent an enema with R. intestinalis as a treatment. The disease activity index, fecal changes and body weight of rats were recorded to evaluate colitis, while histological and immunohistochemical analyses were carried out to examine colon function, and 16S rRNA sequencing was performed to evaluate the gut microbiota change. Behavioral assays and immunohistochemical staining of brain were performed to assess the effect of R. intestinalis on the gut–brain axis. RESULTS: Colitis-related symptoms in rats were significantly relieved after R. intestinalis enema, and the stool traits and colon length of rats were significantly recovered after treatment. The gut epithelial integrity and intestinal barrier were restored in treated rats, as evidenced by the higher expression of Zo-1 in colon tissues, accompanied by the restoration of gut microbiota. Meanwhile, depressive-like behaviors of rats were reduced after treatment, and laboratory experiments on neuronal cells also showed that IL-6, IL-7 and 5-HT were downregulated by R. intestinalis treatment in both serum and brain tissue, while Iba-1 expression was reduced in treated rats. CONCLUSIONS: The administration of R. intestinalis contributes to restoration of the gut microbiota, promoting colon repair and the recovery of gastrointestinal function. These alterations are accompanied by the relief of depressive-like behaviors through a process modulated by the neuronal network and the regulation of inflammation by the gut–brain axis. SAGE Publications 2021-04-15 /pmc/articles/PMC8053823/ /pubmed/33948112 http://dx.doi.org/10.1177/17562848211004469 Text en © The Author(s), 2021 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Research
Xu, Fenghua
Cheng, Yi
Ruan, Guangcong
Fan, Liqin
Tian, Yuting
Xiao, Zhifeng
Chen, Dongfeng
Wei, Yanling
New pathway ameliorating ulcerative colitis: focus on Roseburia intestinalis and the gut–brain axis
title New pathway ameliorating ulcerative colitis: focus on Roseburia intestinalis and the gut–brain axis
title_full New pathway ameliorating ulcerative colitis: focus on Roseburia intestinalis and the gut–brain axis
title_fullStr New pathway ameliorating ulcerative colitis: focus on Roseburia intestinalis and the gut–brain axis
title_full_unstemmed New pathway ameliorating ulcerative colitis: focus on Roseburia intestinalis and the gut–brain axis
title_short New pathway ameliorating ulcerative colitis: focus on Roseburia intestinalis and the gut–brain axis
title_sort new pathway ameliorating ulcerative colitis: focus on roseburia intestinalis and the gut–brain axis
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053823/
https://www.ncbi.nlm.nih.gov/pubmed/33948112
http://dx.doi.org/10.1177/17562848211004469
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