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Combination of platelet rich plasma and stromal vascular fraction on the level of vascular endothelial growth factor in rat subjects experiencing deep dermal burn injury

BACKGROUND: The healing process of burns includes coagulation, inflammation, and remodeling. Vascular endothelial growth factor (VEGF) is involved throughout this healing process. Stem cells from the platelet-rich plasma (PRP) with stromal vascular fraction (SVF) can increase concentrations of growt...

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Autores principales: Laidding, Sachraswaty Rachman, Josh, Fonny, Battung, Sartian, Bukhari, Agussalim, Warsinggih, Patellongi, Ilham Jaya, Massi, Muh Nassrum, Islam, Andi Asadul, Dososaputro, Iswinarno, Faruk, Muhammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053889/
https://www.ncbi.nlm.nih.gov/pubmed/33898027
http://dx.doi.org/10.1016/j.amsu.2021.102254
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author Laidding, Sachraswaty Rachman
Josh, Fonny
Battung, Sartian
Bukhari, Agussalim
Warsinggih
Patellongi, Ilham Jaya
Massi, Muh Nassrum
Islam, Andi Asadul
Dososaputro, Iswinarno
Faruk, Muhammad
author_facet Laidding, Sachraswaty Rachman
Josh, Fonny
Battung, Sartian
Bukhari, Agussalim
Warsinggih
Patellongi, Ilham Jaya
Massi, Muh Nassrum
Islam, Andi Asadul
Dososaputro, Iswinarno
Faruk, Muhammad
author_sort Laidding, Sachraswaty Rachman
collection PubMed
description BACKGROUND: The healing process of burns includes coagulation, inflammation, and remodeling. Vascular endothelial growth factor (VEGF) is involved throughout this healing process. Stem cells from the platelet-rich plasma (PRP) with stromal vascular fraction (SVF) can increase concentrations of growth factors, including VEGF. This is expected to accelerate burn healing. The aim of this study was to determine the effect of a combination of PRP and SVF on VEGF levels in a rats model of deep dermal burn wound healing. MATERIALS AND METHODS: This is an experimental research study in rats using a post-test control group design with 4 groups: A) control, B) Vaseline, C) topical PRP and SVF, and D) PRP and SVF injection. Burn wounds were induced according to the modified Guo method. RESULTS: In a rats model of deep dermal wound healing, topical Vaseline significantly increased serum VEGF compared to control. Topical application and injection of stem cells also significantly increased serum VEGF compared to control and Vaseline. The VEGF concentration was significantly higher following injection of PRP and SVF, suggesting that the injection route is more effective at increasing VEGF levels compared to the topical application of stem cells. CONCLUSION: The combination of PRP and SVF, either by injection or topical application, can increase VEGF levels during the healing process from deep dermal burns.
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spelling pubmed-80538892021-04-22 Combination of platelet rich plasma and stromal vascular fraction on the level of vascular endothelial growth factor in rat subjects experiencing deep dermal burn injury Laidding, Sachraswaty Rachman Josh, Fonny Battung, Sartian Bukhari, Agussalim Warsinggih Patellongi, Ilham Jaya Massi, Muh Nassrum Islam, Andi Asadul Dososaputro, Iswinarno Faruk, Muhammad Ann Med Surg (Lond) Experimental Research BACKGROUND: The healing process of burns includes coagulation, inflammation, and remodeling. Vascular endothelial growth factor (VEGF) is involved throughout this healing process. Stem cells from the platelet-rich plasma (PRP) with stromal vascular fraction (SVF) can increase concentrations of growth factors, including VEGF. This is expected to accelerate burn healing. The aim of this study was to determine the effect of a combination of PRP and SVF on VEGF levels in a rats model of deep dermal burn wound healing. MATERIALS AND METHODS: This is an experimental research study in rats using a post-test control group design with 4 groups: A) control, B) Vaseline, C) topical PRP and SVF, and D) PRP and SVF injection. Burn wounds were induced according to the modified Guo method. RESULTS: In a rats model of deep dermal wound healing, topical Vaseline significantly increased serum VEGF compared to control. Topical application and injection of stem cells also significantly increased serum VEGF compared to control and Vaseline. The VEGF concentration was significantly higher following injection of PRP and SVF, suggesting that the injection route is more effective at increasing VEGF levels compared to the topical application of stem cells. CONCLUSION: The combination of PRP and SVF, either by injection or topical application, can increase VEGF levels during the healing process from deep dermal burns. Elsevier 2021-03-26 /pmc/articles/PMC8053889/ /pubmed/33898027 http://dx.doi.org/10.1016/j.amsu.2021.102254 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Experimental Research
Laidding, Sachraswaty Rachman
Josh, Fonny
Battung, Sartian
Bukhari, Agussalim
Warsinggih
Patellongi, Ilham Jaya
Massi, Muh Nassrum
Islam, Andi Asadul
Dososaputro, Iswinarno
Faruk, Muhammad
Combination of platelet rich plasma and stromal vascular fraction on the level of vascular endothelial growth factor in rat subjects experiencing deep dermal burn injury
title Combination of platelet rich plasma and stromal vascular fraction on the level of vascular endothelial growth factor in rat subjects experiencing deep dermal burn injury
title_full Combination of platelet rich plasma and stromal vascular fraction on the level of vascular endothelial growth factor in rat subjects experiencing deep dermal burn injury
title_fullStr Combination of platelet rich plasma and stromal vascular fraction on the level of vascular endothelial growth factor in rat subjects experiencing deep dermal burn injury
title_full_unstemmed Combination of platelet rich plasma and stromal vascular fraction on the level of vascular endothelial growth factor in rat subjects experiencing deep dermal burn injury
title_short Combination of platelet rich plasma and stromal vascular fraction on the level of vascular endothelial growth factor in rat subjects experiencing deep dermal burn injury
title_sort combination of platelet rich plasma and stromal vascular fraction on the level of vascular endothelial growth factor in rat subjects experiencing deep dermal burn injury
topic Experimental Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053889/
https://www.ncbi.nlm.nih.gov/pubmed/33898027
http://dx.doi.org/10.1016/j.amsu.2021.102254
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