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Establishment of bovine expanded potential stem cells

Embryonic stem cells (ESCs) and induced pluripotent stem cells have the potential to differentiate to all cell types of an adult individual and are useful for studying development and for translational research. However, extrapolation of mouse and human ESC knowledge to deriving stable ESC lines of...

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Autores principales: Zhao, Lixia, Gao, Xuefei, Zheng, Yuxuan, Wang, Zixin, Zhao, Gaoping, Ren, Jie, Zhang, Jia, Wu, Jian, Wu, Baojiang, Chen, Yanglin, Sun, Wei, Li, Yunxia, Su, Jie, Ding, Yulin, Gao, Yuan, Liu, Moning, Bai, Xiaochun, Sun, Liangzhong, Cao, Guifang, Tang, Fuchou, Bao, Siqin, Liu, Pentao, Li, Xihe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053967/
https://www.ncbi.nlm.nih.gov/pubmed/33833056
http://dx.doi.org/10.1073/pnas.2018505118
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author Zhao, Lixia
Gao, Xuefei
Zheng, Yuxuan
Wang, Zixin
Zhao, Gaoping
Ren, Jie
Zhang, Jia
Wu, Jian
Wu, Baojiang
Chen, Yanglin
Sun, Wei
Li, Yunxia
Su, Jie
Ding, Yulin
Gao, Yuan
Liu, Moning
Bai, Xiaochun
Sun, Liangzhong
Cao, Guifang
Tang, Fuchou
Bao, Siqin
Liu, Pentao
Li, Xihe
author_facet Zhao, Lixia
Gao, Xuefei
Zheng, Yuxuan
Wang, Zixin
Zhao, Gaoping
Ren, Jie
Zhang, Jia
Wu, Jian
Wu, Baojiang
Chen, Yanglin
Sun, Wei
Li, Yunxia
Su, Jie
Ding, Yulin
Gao, Yuan
Liu, Moning
Bai, Xiaochun
Sun, Liangzhong
Cao, Guifang
Tang, Fuchou
Bao, Siqin
Liu, Pentao
Li, Xihe
author_sort Zhao, Lixia
collection PubMed
description Embryonic stem cells (ESCs) and induced pluripotent stem cells have the potential to differentiate to all cell types of an adult individual and are useful for studying development and for translational research. However, extrapolation of mouse and human ESC knowledge to deriving stable ESC lines of domestic ungulates and large livestock species has been challenging. In contrast to ESCs that are usually established from the blastocyst, mouse expanded potential stem cells (EPSCs) are derived from four-cell and eight-cell embryos. We have recently used the EPSC approach and established stem cells from porcine and human preimplantation embryos. EPSCs are molecularly similar across species and have broader developmental potential to generate embryonic and extraembryonic cell lineages. We further explore the EPSC technology for mammalian species refractory to the standard ESC approaches and report here the successful establishment of bovine EPSCs (bEPSCs) from preimplantation embryos of both wild-type and somatic cell nuclear transfer. bEPSCs express high levels of pluripotency genes, propagate robustly in feeder-free culture, and are genetically stable in long-term culture. bEPSCs have enriched transcriptomic features of early preimplantation embryos and differentiate in vitro to cells of the three somatic germ layers and, in chimeras, contribute to both the embryonic (fetal) and extraembryonic cell lineages. Importantly, precise gene editing is efficiently achieved in bEPSCs, and genetically modified bEPSCs can be used as donors in somatic cell nuclear transfer. bEPSCs therefore hold the potential to substantially advance biotechnology and agriculture.
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spelling pubmed-80539672021-05-04 Establishment of bovine expanded potential stem cells Zhao, Lixia Gao, Xuefei Zheng, Yuxuan Wang, Zixin Zhao, Gaoping Ren, Jie Zhang, Jia Wu, Jian Wu, Baojiang Chen, Yanglin Sun, Wei Li, Yunxia Su, Jie Ding, Yulin Gao, Yuan Liu, Moning Bai, Xiaochun Sun, Liangzhong Cao, Guifang Tang, Fuchou Bao, Siqin Liu, Pentao Li, Xihe Proc Natl Acad Sci U S A Biological Sciences Embryonic stem cells (ESCs) and induced pluripotent stem cells have the potential to differentiate to all cell types of an adult individual and are useful for studying development and for translational research. However, extrapolation of mouse and human ESC knowledge to deriving stable ESC lines of domestic ungulates and large livestock species has been challenging. In contrast to ESCs that are usually established from the blastocyst, mouse expanded potential stem cells (EPSCs) are derived from four-cell and eight-cell embryos. We have recently used the EPSC approach and established stem cells from porcine and human preimplantation embryos. EPSCs are molecularly similar across species and have broader developmental potential to generate embryonic and extraembryonic cell lineages. We further explore the EPSC technology for mammalian species refractory to the standard ESC approaches and report here the successful establishment of bovine EPSCs (bEPSCs) from preimplantation embryos of both wild-type and somatic cell nuclear transfer. bEPSCs express high levels of pluripotency genes, propagate robustly in feeder-free culture, and are genetically stable in long-term culture. bEPSCs have enriched transcriptomic features of early preimplantation embryos and differentiate in vitro to cells of the three somatic germ layers and, in chimeras, contribute to both the embryonic (fetal) and extraembryonic cell lineages. Importantly, precise gene editing is efficiently achieved in bEPSCs, and genetically modified bEPSCs can be used as donors in somatic cell nuclear transfer. bEPSCs therefore hold the potential to substantially advance biotechnology and agriculture. National Academy of Sciences 2021-04-13 2021-04-08 /pmc/articles/PMC8053967/ /pubmed/33833056 http://dx.doi.org/10.1073/pnas.2018505118 Text en Copyright © 2021 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by/4.0/This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Biological Sciences
Zhao, Lixia
Gao, Xuefei
Zheng, Yuxuan
Wang, Zixin
Zhao, Gaoping
Ren, Jie
Zhang, Jia
Wu, Jian
Wu, Baojiang
Chen, Yanglin
Sun, Wei
Li, Yunxia
Su, Jie
Ding, Yulin
Gao, Yuan
Liu, Moning
Bai, Xiaochun
Sun, Liangzhong
Cao, Guifang
Tang, Fuchou
Bao, Siqin
Liu, Pentao
Li, Xihe
Establishment of bovine expanded potential stem cells
title Establishment of bovine expanded potential stem cells
title_full Establishment of bovine expanded potential stem cells
title_fullStr Establishment of bovine expanded potential stem cells
title_full_unstemmed Establishment of bovine expanded potential stem cells
title_short Establishment of bovine expanded potential stem cells
title_sort establishment of bovine expanded potential stem cells
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053967/
https://www.ncbi.nlm.nih.gov/pubmed/33833056
http://dx.doi.org/10.1073/pnas.2018505118
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