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Atmospheric pressure chemical ionisation mass spectrometry for the routine analysis of low molecular weight analytes

The routine analysis of low molecular weight analytes by mass spectrometry is often complicated by the lability of the analyte’s functional groups and/or the lack of moieties that can be easily charged. If a molecule is too labile this precludes analysis by techniques such as electron ionisation or...

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Autor principal: Gates, Paul J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054169/
https://www.ncbi.nlm.nih.gov/pubmed/33820464
http://dx.doi.org/10.1177/14690667211005055
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author Gates, Paul J
author_facet Gates, Paul J
author_sort Gates, Paul J
collection PubMed
description The routine analysis of low molecular weight analytes by mass spectrometry is often complicated by the lability of the analyte’s functional groups and/or the lack of moieties that can be easily charged. If a molecule is too labile this precludes analysis by techniques such as electron ionisation or chemical ionisation as the analyte will undergo thermal decomposition prior to ionisation as well as spontaneous fragmentation during the ionisation process. If the analyte has a low propensity to form ions in electrospray ionisation (i.e., lacks acidic or basic sites) then often no analyte related ions are observed. In this paper, the robustness and versatility of the established method of atmospheric pressure chemical ionisation is demonstrated for the analysis of low molecular weight analytes. The utility of the technique is demonstrated through the analysis of 30 reference standards of varying functionality, and further by the analysis of 75 synthetic samples which were problematic when analysed by electron or electrospray ionisation. The resulting spectra are dominated by intact molecular species ([M+H](+) and M(+) in positive ion mode and [M − H](−) and [M + Cl](−) in negative ion mode) along with logical neutral losses reminiscent of what you might expect from the analyte’s structure (losses of H(2)O from alcohols or CO from aldehydes etc). This paper presents atmospheric pressure chemical ionisation as an essential tool for broadening the chemical space of successful analyses for any routine mass spectrometry service laboratory of facility.
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spelling pubmed-80541692021-05-04 Atmospheric pressure chemical ionisation mass spectrometry for the routine analysis of low molecular weight analytes Gates, Paul J Eur J Mass Spectrom (Chichester) Original Research Papers The routine analysis of low molecular weight analytes by mass spectrometry is often complicated by the lability of the analyte’s functional groups and/or the lack of moieties that can be easily charged. If a molecule is too labile this precludes analysis by techniques such as electron ionisation or chemical ionisation as the analyte will undergo thermal decomposition prior to ionisation as well as spontaneous fragmentation during the ionisation process. If the analyte has a low propensity to form ions in electrospray ionisation (i.e., lacks acidic or basic sites) then often no analyte related ions are observed. In this paper, the robustness and versatility of the established method of atmospheric pressure chemical ionisation is demonstrated for the analysis of low molecular weight analytes. The utility of the technique is demonstrated through the analysis of 30 reference standards of varying functionality, and further by the analysis of 75 synthetic samples which were problematic when analysed by electron or electrospray ionisation. The resulting spectra are dominated by intact molecular species ([M+H](+) and M(+) in positive ion mode and [M − H](−) and [M + Cl](−) in negative ion mode) along with logical neutral losses reminiscent of what you might expect from the analyte’s structure (losses of H(2)O from alcohols or CO from aldehydes etc). This paper presents atmospheric pressure chemical ionisation as an essential tool for broadening the chemical space of successful analyses for any routine mass spectrometry service laboratory of facility. SAGE Publications 2021-04-05 2021-02 /pmc/articles/PMC8054169/ /pubmed/33820464 http://dx.doi.org/10.1177/14690667211005055 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Research Papers
Gates, Paul J
Atmospheric pressure chemical ionisation mass spectrometry for the routine analysis of low molecular weight analytes
title Atmospheric pressure chemical ionisation mass spectrometry for the routine analysis of low molecular weight analytes
title_full Atmospheric pressure chemical ionisation mass spectrometry for the routine analysis of low molecular weight analytes
title_fullStr Atmospheric pressure chemical ionisation mass spectrometry for the routine analysis of low molecular weight analytes
title_full_unstemmed Atmospheric pressure chemical ionisation mass spectrometry for the routine analysis of low molecular weight analytes
title_short Atmospheric pressure chemical ionisation mass spectrometry for the routine analysis of low molecular weight analytes
title_sort atmospheric pressure chemical ionisation mass spectrometry for the routine analysis of low molecular weight analytes
topic Original Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054169/
https://www.ncbi.nlm.nih.gov/pubmed/33820464
http://dx.doi.org/10.1177/14690667211005055
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