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Evaluation of hTERT, KRT7, and survivin in urine for noninvasive detection of bladder cancer using real‐time PCR

BACKGROUND: Transitional cell carcinoma (TCC) of the bladder is the second most common genitourinary malignancy. Because of the low sensitivity of urinary cytology and the invasiveness and expense of frequent cystoscopies for the detection of low-grade superficial lesions, we aim to establish a sens...

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Autores principales: Yahyazadeh, Reza, Bashash, Davood, Ghaffari, Parisa, Kord, Saeid, Safaroghli-Azar, Ava, Ghaffari, Seyed H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054358/
https://www.ncbi.nlm.nih.gov/pubmed/33874920
http://dx.doi.org/10.1186/s12894-021-00838-z
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author Yahyazadeh, Reza
Bashash, Davood
Ghaffari, Parisa
Kord, Saeid
Safaroghli-Azar, Ava
Ghaffari, Seyed H.
author_facet Yahyazadeh, Reza
Bashash, Davood
Ghaffari, Parisa
Kord, Saeid
Safaroghli-Azar, Ava
Ghaffari, Seyed H.
author_sort Yahyazadeh, Reza
collection PubMed
description BACKGROUND: Transitional cell carcinoma (TCC) of the bladder is the second most common genitourinary malignancy. Because of the low sensitivity of urinary cytology and the invasiveness and expense of frequent cystoscopies for the detection of low-grade superficial lesions, we aim to establish a sensitive molecular approach to detect bladder cancer noninvasively. METHODS: Voided urine samples were collected from 80 patients with bladder cancer at the time of diagnosis, in addition to 30 patients with non-bladder cancer urological diseases and 20 healthy volunteers. The level of hTERT, KRT7, and survivin (SVV) mRNAs were analyzed using a qRT-PCR assay. RESULTS: The optimal threshold values for hTERT, KRT7, and SVV in urine were calculated by ROC curves analysis. The overall sensitivity was 81.3%, 91.3%, and 68.8% for hTERT, KRT7, and SVV, respectively, which were significantly higher than urine cytology (22.2%, p < 0.001). A higher positive ratio was obtained using multi-marker detection in comparison to single marker detection. The combined use of markers increased the sensitivity of cytology from 22.2 to 100%. In contrast with the urine cytology method, the sensitivity of these biomarkers was not correlated with the grades and stages of the bladder tumors. CONCLUSIONS: Our data indicate that urinary hTERT, KRT7, and SVV have superior sensitivities over cytology. The combined use of these markers offers a powerful potential assay and promising tool for a sensitive, noninvasive, and highly specific diagnostic method and follow-up of low-grade TCC of the bladder.
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spelling pubmed-80543582021-04-20 Evaluation of hTERT, KRT7, and survivin in urine for noninvasive detection of bladder cancer using real‐time PCR Yahyazadeh, Reza Bashash, Davood Ghaffari, Parisa Kord, Saeid Safaroghli-Azar, Ava Ghaffari, Seyed H. BMC Urol Research BACKGROUND: Transitional cell carcinoma (TCC) of the bladder is the second most common genitourinary malignancy. Because of the low sensitivity of urinary cytology and the invasiveness and expense of frequent cystoscopies for the detection of low-grade superficial lesions, we aim to establish a sensitive molecular approach to detect bladder cancer noninvasively. METHODS: Voided urine samples were collected from 80 patients with bladder cancer at the time of diagnosis, in addition to 30 patients with non-bladder cancer urological diseases and 20 healthy volunteers. The level of hTERT, KRT7, and survivin (SVV) mRNAs were analyzed using a qRT-PCR assay. RESULTS: The optimal threshold values for hTERT, KRT7, and SVV in urine were calculated by ROC curves analysis. The overall sensitivity was 81.3%, 91.3%, and 68.8% for hTERT, KRT7, and SVV, respectively, which were significantly higher than urine cytology (22.2%, p < 0.001). A higher positive ratio was obtained using multi-marker detection in comparison to single marker detection. The combined use of markers increased the sensitivity of cytology from 22.2 to 100%. In contrast with the urine cytology method, the sensitivity of these biomarkers was not correlated with the grades and stages of the bladder tumors. CONCLUSIONS: Our data indicate that urinary hTERT, KRT7, and SVV have superior sensitivities over cytology. The combined use of these markers offers a powerful potential assay and promising tool for a sensitive, noninvasive, and highly specific diagnostic method and follow-up of low-grade TCC of the bladder. BioMed Central 2021-04-19 /pmc/articles/PMC8054358/ /pubmed/33874920 http://dx.doi.org/10.1186/s12894-021-00838-z Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Yahyazadeh, Reza
Bashash, Davood
Ghaffari, Parisa
Kord, Saeid
Safaroghli-Azar, Ava
Ghaffari, Seyed H.
Evaluation of hTERT, KRT7, and survivin in urine for noninvasive detection of bladder cancer using real‐time PCR
title Evaluation of hTERT, KRT7, and survivin in urine for noninvasive detection of bladder cancer using real‐time PCR
title_full Evaluation of hTERT, KRT7, and survivin in urine for noninvasive detection of bladder cancer using real‐time PCR
title_fullStr Evaluation of hTERT, KRT7, and survivin in urine for noninvasive detection of bladder cancer using real‐time PCR
title_full_unstemmed Evaluation of hTERT, KRT7, and survivin in urine for noninvasive detection of bladder cancer using real‐time PCR
title_short Evaluation of hTERT, KRT7, and survivin in urine for noninvasive detection of bladder cancer using real‐time PCR
title_sort evaluation of htert, krt7, and survivin in urine for noninvasive detection of bladder cancer using real‐time pcr
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054358/
https://www.ncbi.nlm.nih.gov/pubmed/33874920
http://dx.doi.org/10.1186/s12894-021-00838-z
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