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The clinical significance of UBE2C gene in progression of renal cell carcinoma
Renal cell carcinoma (RCC), with high morbidity and mortality, is one of the top ten serious cancers. Due to limited therapies and little knowledge about the mechanism underlying RCC, overall survival of RCC patients is poor. UBE2C is a member of ubiquitin modification system and promotes carcinogen...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PAGEPress Publications, Pavia, Italy
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054571/ https://www.ncbi.nlm.nih.gov/pubmed/33782624 http://dx.doi.org/10.4081/ejh.2021.3196 |
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author | Chen, Zhiping Wang, Lanfeng |
author_facet | Chen, Zhiping Wang, Lanfeng |
author_sort | Chen, Zhiping |
collection | PubMed |
description | Renal cell carcinoma (RCC), with high morbidity and mortality, is one of the top ten serious cancers. Due to limited therapies and little knowledge about the mechanism underlying RCC, overall survival of RCC patients is poor. UBE2C is a member of ubiquitin modification system and promotes carcinogenesis in cancer, but its role in RCC is unknown. Based on the TCGA (The Cancer Genome Atlas) data, UBE2C was over-expressed in a total of 525 RCC tissues and displayed higher expression in advanced tissues (stage IV vs stage I, p<0.05). RT-qPCR and IHC analysis confirmed over-expression of UBE2C in 90 of clinical RCC tissues. Further, UBE2C was associated with clinical factors including TNM stage, gender, and pathological stage. And higher UBE2C expression predicted shorter overall survival and progression-free survival. Both univariate and multivariate COX analysis suggested UBE2C as a critical gene in RCC. Then GO and KEGG analysis showed that cell cycle and DNA replication pathways were two top signaling pathways affected by UBE2C. In vitro assay showed that knockdown of UBE2C in 786-O cells inhibited proliferation and migration significantly. Therefore, this study proves that UBE2C is an important gene in RCC and is essential to proliferation and migration of RCC. |
format | Online Article Text |
id | pubmed-8054571 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | PAGEPress Publications, Pavia, Italy |
record_format | MEDLINE/PubMed |
spelling | pubmed-80545712021-04-22 The clinical significance of UBE2C gene in progression of renal cell carcinoma Chen, Zhiping Wang, Lanfeng Eur J Histochem Article Renal cell carcinoma (RCC), with high morbidity and mortality, is one of the top ten serious cancers. Due to limited therapies and little knowledge about the mechanism underlying RCC, overall survival of RCC patients is poor. UBE2C is a member of ubiquitin modification system and promotes carcinogenesis in cancer, but its role in RCC is unknown. Based on the TCGA (The Cancer Genome Atlas) data, UBE2C was over-expressed in a total of 525 RCC tissues and displayed higher expression in advanced tissues (stage IV vs stage I, p<0.05). RT-qPCR and IHC analysis confirmed over-expression of UBE2C in 90 of clinical RCC tissues. Further, UBE2C was associated with clinical factors including TNM stage, gender, and pathological stage. And higher UBE2C expression predicted shorter overall survival and progression-free survival. Both univariate and multivariate COX analysis suggested UBE2C as a critical gene in RCC. Then GO and KEGG analysis showed that cell cycle and DNA replication pathways were two top signaling pathways affected by UBE2C. In vitro assay showed that knockdown of UBE2C in 786-O cells inhibited proliferation and migration significantly. Therefore, this study proves that UBE2C is an important gene in RCC and is essential to proliferation and migration of RCC. PAGEPress Publications, Pavia, Italy 2021-03-29 /pmc/articles/PMC8054571/ /pubmed/33782624 http://dx.doi.org/10.4081/ejh.2021.3196 Text en ©Copyright: the Author(s) https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution Noncommercial License (by-nc 4.0) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Article Chen, Zhiping Wang, Lanfeng The clinical significance of UBE2C gene in progression of renal cell carcinoma |
title | The clinical significance of UBE2C gene in progression of renal cell carcinoma |
title_full | The clinical significance of UBE2C gene in progression of renal cell carcinoma |
title_fullStr | The clinical significance of UBE2C gene in progression of renal cell carcinoma |
title_full_unstemmed | The clinical significance of UBE2C gene in progression of renal cell carcinoma |
title_short | The clinical significance of UBE2C gene in progression of renal cell carcinoma |
title_sort | clinical significance of ube2c gene in progression of renal cell carcinoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054571/ https://www.ncbi.nlm.nih.gov/pubmed/33782624 http://dx.doi.org/10.4081/ejh.2021.3196 |
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