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Genetic markers for preeclampsia in Peruvian women
BACKGROUND: Preeclampsia is a multiorgan disorder associated with maternal and perinatal morbi-mortality. In Peru, incidence is 10% and accounts for 22% of maternal deaths. Genome and genetic epidemiological studies have found an association between preeclampsia and genetic polymorphisms. OBJECTIVE:...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Universidad del Valle
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054708/ https://www.ncbi.nlm.nih.gov/pubmed/33911318 http://dx.doi.org/10.25100/cm.v52i1.4437 |
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author | Pacheco-Romero, José Acosta, Oscar Huerta, Doris Cabrera, Santiago Vargas, Marlene Mascaro, Pedro Huamán, Moisés Sandoval, José López, Rudy Mateus, Julio Gil, Enrique Guevara, Enrique Butrica, Nitza Catari, Diana Bellido, David Custodio, Gina Naranjo, Andrea |
author_facet | Pacheco-Romero, José Acosta, Oscar Huerta, Doris Cabrera, Santiago Vargas, Marlene Mascaro, Pedro Huamán, Moisés Sandoval, José López, Rudy Mateus, Julio Gil, Enrique Guevara, Enrique Butrica, Nitza Catari, Diana Bellido, David Custodio, Gina Naranjo, Andrea |
author_sort | Pacheco-Romero, José |
collection | PubMed |
description | BACKGROUND: Preeclampsia is a multiorgan disorder associated with maternal and perinatal morbi-mortality. In Peru, incidence is 10% and accounts for 22% of maternal deaths. Genome and genetic epidemiological studies have found an association between preeclampsia and genetic polymorphisms. OBJECTIVE: To determine the association of the vascular endothelial growth factor (VEGF) +936 C/T and +405 G/C, interleukine-6 (IL-6) -174 G/C, IL-1β-511 C/T, Apo A-1-75 G/A, Apo B-100 2488 C/T (Xbal) polymorphisms with preeclampsia in pregnant Peruvian women. METHODS: Were included preeclamptic and healthy (control) pregnant women. Maternal blood samples were subjected to DNA extraction, and molecular genetic analysis was conducted using the PCR-RFLP technique and following a specific protocol for each gene. Allele and genotypic frequencies in the cases and controls were compared. RESULTS: No association was found between the VEGF+936C/T and VEGF+405 polymorphisms and preeclampsia. The frequencies of the GG genotypes and the G allele of the -174 G/C polymorphism in the IL6 gene in preeclamptic and controls showed significant differences, with higher frequencies in cases. For the -511 C/T polymorphism of the IL-1β gene, no significant differences were found in the frequencies of TT genotypes compared with CT+CC. The genotypes and alleles of the Apo-A1-75 G/A and Apo-B100 Xbal variants showed no significant differences between cases and controls. CONCLUSION: No association was found between the studied genetic markers and preeclampsia. However, in the -174G/C polymorphism of the IL-6 gene, significant differences were found mainly in the GG genotype and G allele. |
format | Online Article Text |
id | pubmed-8054708 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Universidad del Valle |
record_format | MEDLINE/PubMed |
spelling | pubmed-80547082021-04-27 Genetic markers for preeclampsia in Peruvian women Pacheco-Romero, José Acosta, Oscar Huerta, Doris Cabrera, Santiago Vargas, Marlene Mascaro, Pedro Huamán, Moisés Sandoval, José López, Rudy Mateus, Julio Gil, Enrique Guevara, Enrique Butrica, Nitza Catari, Diana Bellido, David Custodio, Gina Naranjo, Andrea Colomb Med (Cali) Original Article BACKGROUND: Preeclampsia is a multiorgan disorder associated with maternal and perinatal morbi-mortality. In Peru, incidence is 10% and accounts for 22% of maternal deaths. Genome and genetic epidemiological studies have found an association between preeclampsia and genetic polymorphisms. OBJECTIVE: To determine the association of the vascular endothelial growth factor (VEGF) +936 C/T and +405 G/C, interleukine-6 (IL-6) -174 G/C, IL-1β-511 C/T, Apo A-1-75 G/A, Apo B-100 2488 C/T (Xbal) polymorphisms with preeclampsia in pregnant Peruvian women. METHODS: Were included preeclamptic and healthy (control) pregnant women. Maternal blood samples were subjected to DNA extraction, and molecular genetic analysis was conducted using the PCR-RFLP technique and following a specific protocol for each gene. Allele and genotypic frequencies in the cases and controls were compared. RESULTS: No association was found between the VEGF+936C/T and VEGF+405 polymorphisms and preeclampsia. The frequencies of the GG genotypes and the G allele of the -174 G/C polymorphism in the IL6 gene in preeclamptic and controls showed significant differences, with higher frequencies in cases. For the -511 C/T polymorphism of the IL-1β gene, no significant differences were found in the frequencies of TT genotypes compared with CT+CC. The genotypes and alleles of the Apo-A1-75 G/A and Apo-B100 Xbal variants showed no significant differences between cases and controls. CONCLUSION: No association was found between the studied genetic markers and preeclampsia. However, in the -174G/C polymorphism of the IL-6 gene, significant differences were found mainly in the GG genotype and G allele. Universidad del Valle 2021-02-26 /pmc/articles/PMC8054708/ /pubmed/33911318 http://dx.doi.org/10.25100/cm.v52i1.4437 Text en Copyright © 2021 Colombia Medica https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Original Article Pacheco-Romero, José Acosta, Oscar Huerta, Doris Cabrera, Santiago Vargas, Marlene Mascaro, Pedro Huamán, Moisés Sandoval, José López, Rudy Mateus, Julio Gil, Enrique Guevara, Enrique Butrica, Nitza Catari, Diana Bellido, David Custodio, Gina Naranjo, Andrea Genetic markers for preeclampsia in Peruvian women |
title | Genetic markers for preeclampsia in Peruvian women |
title_full | Genetic markers for preeclampsia in Peruvian women |
title_fullStr | Genetic markers for preeclampsia in Peruvian women |
title_full_unstemmed | Genetic markers for preeclampsia in Peruvian women |
title_short | Genetic markers for preeclampsia in Peruvian women |
title_sort | genetic markers for preeclampsia in peruvian women |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054708/ https://www.ncbi.nlm.nih.gov/pubmed/33911318 http://dx.doi.org/10.25100/cm.v52i1.4437 |
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