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Sequence analysis of new variants of porcine epidemic diarrhea virus in Luzon, Philippines, in 2017

Porcine epidemic diarrhea virus (PEDV) is a coronavirus that causes emaciation and watery diarrhea in pigs. First identified in Europe in 1977, it eventually spread to Asia and North America, causing deadly outbreaks in neonatal piglets. In the Philippines, PEDV has caused several recorded outbreaks...

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Autores principales: Salamat, Saubel Ezrael A., Collantes, Therese Marie A., Lumbera, Wenchie Marie L., Tablizo, Francis A., Mutia, Christian Thomas M., Ong, John Daniel P., Bandoy, D J Darwin R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055054/
https://www.ncbi.nlm.nih.gov/pubmed/33876315
http://dx.doi.org/10.1007/s00705-021-05068-0
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author Salamat, Saubel Ezrael A.
Collantes, Therese Marie A.
Lumbera, Wenchie Marie L.
Tablizo, Francis A.
Mutia, Christian Thomas M.
Ong, John Daniel P.
Bandoy, D J Darwin R.
author_facet Salamat, Saubel Ezrael A.
Collantes, Therese Marie A.
Lumbera, Wenchie Marie L.
Tablizo, Francis A.
Mutia, Christian Thomas M.
Ong, John Daniel P.
Bandoy, D J Darwin R.
author_sort Salamat, Saubel Ezrael A.
collection PubMed
description Porcine epidemic diarrhea virus (PEDV) is a coronavirus that causes emaciation and watery diarrhea in pigs. First identified in Europe in 1977, it eventually spread to Asia and North America, causing deadly outbreaks in neonatal piglets. In the Philippines, PEDV has caused several recorded outbreaks since 2005. However, DNA sequencing studies of local PEDV strains remain few and are limited to gene and gene fragment sequencing. Therefore, to provide updated sequence information about recent PEDV strains in the country, we performed reverse transcription PCR and sequencing of PEDV from swab samples collected from swine farms in the Philippines in 2017. Here, we report the first published whole genome sequence of PEDV from the Philippines as well as CO-26K equivalent (COE) domain sequences of strains from three provinces in Luzon where PEDV was detected in 2017. Sequence analysis suggested that PEDV from both the classical (genotype 1) and pandemic (genotype 2) groups are present in the Philippines, with possible East Asian and North American origins.
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spelling pubmed-80550542021-04-20 Sequence analysis of new variants of porcine epidemic diarrhea virus in Luzon, Philippines, in 2017 Salamat, Saubel Ezrael A. Collantes, Therese Marie A. Lumbera, Wenchie Marie L. Tablizo, Francis A. Mutia, Christian Thomas M. Ong, John Daniel P. Bandoy, D J Darwin R. Arch Virol Original Article Porcine epidemic diarrhea virus (PEDV) is a coronavirus that causes emaciation and watery diarrhea in pigs. First identified in Europe in 1977, it eventually spread to Asia and North America, causing deadly outbreaks in neonatal piglets. In the Philippines, PEDV has caused several recorded outbreaks since 2005. However, DNA sequencing studies of local PEDV strains remain few and are limited to gene and gene fragment sequencing. Therefore, to provide updated sequence information about recent PEDV strains in the country, we performed reverse transcription PCR and sequencing of PEDV from swab samples collected from swine farms in the Philippines in 2017. Here, we report the first published whole genome sequence of PEDV from the Philippines as well as CO-26K equivalent (COE) domain sequences of strains from three provinces in Luzon where PEDV was detected in 2017. Sequence analysis suggested that PEDV from both the classical (genotype 1) and pandemic (genotype 2) groups are present in the Philippines, with possible East Asian and North American origins. Springer Vienna 2021-04-19 2021 /pmc/articles/PMC8055054/ /pubmed/33876315 http://dx.doi.org/10.1007/s00705-021-05068-0 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Article
Salamat, Saubel Ezrael A.
Collantes, Therese Marie A.
Lumbera, Wenchie Marie L.
Tablizo, Francis A.
Mutia, Christian Thomas M.
Ong, John Daniel P.
Bandoy, D J Darwin R.
Sequence analysis of new variants of porcine epidemic diarrhea virus in Luzon, Philippines, in 2017
title Sequence analysis of new variants of porcine epidemic diarrhea virus in Luzon, Philippines, in 2017
title_full Sequence analysis of new variants of porcine epidemic diarrhea virus in Luzon, Philippines, in 2017
title_fullStr Sequence analysis of new variants of porcine epidemic diarrhea virus in Luzon, Philippines, in 2017
title_full_unstemmed Sequence analysis of new variants of porcine epidemic diarrhea virus in Luzon, Philippines, in 2017
title_short Sequence analysis of new variants of porcine epidemic diarrhea virus in Luzon, Philippines, in 2017
title_sort sequence analysis of new variants of porcine epidemic diarrhea virus in luzon, philippines, in 2017
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055054/
https://www.ncbi.nlm.nih.gov/pubmed/33876315
http://dx.doi.org/10.1007/s00705-021-05068-0
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