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Green, Cost-Effective Simultaneous Assay of Chloramphenicol, Methylparaben, and Propylparaben in Eye-Drops by Capillary Zone Electrophoresis

A green, cost-effective, and simple capillary zone electrophoresis (CZE) method was developed and validated for simultaneous determination of chloramphenicol, methylparaben, and propylparaben in eye-drops. With sodium tetraborate as background electrolyte (BGE), the apparent mobilities of chloramphe...

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Autores principales: Tong, Thi Thanh Vuong, Cao, Thi Thoa, Tran, Nguyen Ha, Le, Thi Kim Van, Le, Dinh Chi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055384/
https://www.ncbi.nlm.nih.gov/pubmed/33936836
http://dx.doi.org/10.1155/2021/5575701
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author Tong, Thi Thanh Vuong
Cao, Thi Thoa
Tran, Nguyen Ha
Le, Thi Kim Van
Le, Dinh Chi
author_facet Tong, Thi Thanh Vuong
Cao, Thi Thoa
Tran, Nguyen Ha
Le, Thi Kim Van
Le, Dinh Chi
author_sort Tong, Thi Thanh Vuong
collection PubMed
description A green, cost-effective, and simple capillary zone electrophoresis (CZE) method was developed and validated for simultaneous determination of chloramphenicol, methylparaben, and propylparaben in eye-drops. With sodium tetraborate as background electrolyte (BGE), the apparent mobilities of chloramphenicol, methylparaben, and propylparaben increased and analysis time reduced when pH of BGE increased from 8.5 to 10.0 and concentration of BGE decreased from 40 mM to 15 mM, but complete separation of chloramphenicol from other matrix components was achieved only with sodium tetraborate concentration at 30 mM or higher and at pH = 9.3 or lower. The most suitable electrophoretic conditions for the intended application were a 30 mM sodium tetraborate solution, pH 9.3 as BGE, working voltage set at 25 kV, and UV detection at 280 nm at the cathodic extremity of the capillary. The final method was validated and proved to be reliable for assay of chloramphenicol, methylparaben, and propylparaben in eye-drops.
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spelling pubmed-80553842021-04-29 Green, Cost-Effective Simultaneous Assay of Chloramphenicol, Methylparaben, and Propylparaben in Eye-Drops by Capillary Zone Electrophoresis Tong, Thi Thanh Vuong Cao, Thi Thoa Tran, Nguyen Ha Le, Thi Kim Van Le, Dinh Chi J Anal Methods Chem Research Article A green, cost-effective, and simple capillary zone electrophoresis (CZE) method was developed and validated for simultaneous determination of chloramphenicol, methylparaben, and propylparaben in eye-drops. With sodium tetraborate as background electrolyte (BGE), the apparent mobilities of chloramphenicol, methylparaben, and propylparaben increased and analysis time reduced when pH of BGE increased from 8.5 to 10.0 and concentration of BGE decreased from 40 mM to 15 mM, but complete separation of chloramphenicol from other matrix components was achieved only with sodium tetraborate concentration at 30 mM or higher and at pH = 9.3 or lower. The most suitable electrophoretic conditions for the intended application were a 30 mM sodium tetraborate solution, pH 9.3 as BGE, working voltage set at 25 kV, and UV detection at 280 nm at the cathodic extremity of the capillary. The final method was validated and proved to be reliable for assay of chloramphenicol, methylparaben, and propylparaben in eye-drops. Hindawi 2021-04-10 /pmc/articles/PMC8055384/ /pubmed/33936836 http://dx.doi.org/10.1155/2021/5575701 Text en Copyright © 2021 Thi Thanh Vuong Tong et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tong, Thi Thanh Vuong
Cao, Thi Thoa
Tran, Nguyen Ha
Le, Thi Kim Van
Le, Dinh Chi
Green, Cost-Effective Simultaneous Assay of Chloramphenicol, Methylparaben, and Propylparaben in Eye-Drops by Capillary Zone Electrophoresis
title Green, Cost-Effective Simultaneous Assay of Chloramphenicol, Methylparaben, and Propylparaben in Eye-Drops by Capillary Zone Electrophoresis
title_full Green, Cost-Effective Simultaneous Assay of Chloramphenicol, Methylparaben, and Propylparaben in Eye-Drops by Capillary Zone Electrophoresis
title_fullStr Green, Cost-Effective Simultaneous Assay of Chloramphenicol, Methylparaben, and Propylparaben in Eye-Drops by Capillary Zone Electrophoresis
title_full_unstemmed Green, Cost-Effective Simultaneous Assay of Chloramphenicol, Methylparaben, and Propylparaben in Eye-Drops by Capillary Zone Electrophoresis
title_short Green, Cost-Effective Simultaneous Assay of Chloramphenicol, Methylparaben, and Propylparaben in Eye-Drops by Capillary Zone Electrophoresis
title_sort green, cost-effective simultaneous assay of chloramphenicol, methylparaben, and propylparaben in eye-drops by capillary zone electrophoresis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055384/
https://www.ncbi.nlm.nih.gov/pubmed/33936836
http://dx.doi.org/10.1155/2021/5575701
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