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Inhibition of defensin A and cecropin A responses to dengue virus 1 infection in Aedes aegypti
INTRODUCTION: It is essential to determine the interactions between viruses and mosquitoes to diminish dengue viral transmission. These interactions constitute a very complex system of highly regulated pathways known as the innate immune system of the mosquito, which produces antimicrobial peptides...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Biteca
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055593/ https://www.ncbi.nlm.nih.gov/pubmed/33761199 http://dx.doi.org/10.7705/biomedica.5491 |
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author | Méndez, Yda Pacheco, César Herrera, Flor |
author_facet | Méndez, Yda Pacheco, César Herrera, Flor |
author_sort | Méndez, Yda |
collection | PubMed |
description | INTRODUCTION: It is essential to determine the interactions between viruses and mosquitoes to diminish dengue viral transmission. These interactions constitute a very complex system of highly regulated pathways known as the innate immune system of the mosquito, which produces antimicrobial peptides that act as effector molecules against bacterial and fungal infections. There is less information about such effects on virus infections. OBJECTIVE: To determine the expression of two antimicrobial peptide genes, defensin A and cecropin A, in Aedes aegypti mosquitoes infected with DENV-1. MATERIALS AND METHODS: We used the F(1) generation of mosquitoes orally infected with DENV-1 and real-time PCR analysis to determine whether the defensin A and cecropin A genes played a role in controlling DENV-1 replication in Ae. aegypti. As a reference, we conducted similar experiments with the bacteria Escherichia coli. RESULTS: Basal levels of defensin A and cecropin A mRNA were expressed in uninfected mosquitoes at different times post-blood feeding. The infected mosquitoes experienced reduced expression of these mRNA by at least eightfold when compared to uninfected control mosquitoes at all times post-infection. In contrast with the behavior of DENV-1, results showed that bacterial infection produced up-regulation of defensin and cecropin genes; however, the induction of transcripts occurred at later times (15 days). Conclusion: DENV-1 virus inhibited the expression of defensin A and cecropin A genes in a wild Ae. aegypti population from Venezuela. |
format | Online Article Text |
id | pubmed-8055593 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Biteca |
record_format | MEDLINE/PubMed |
spelling | pubmed-80555932021-04-20 Inhibition of defensin A and cecropin A responses to dengue virus 1 infection in Aedes aegypti Méndez, Yda Pacheco, César Herrera, Flor Biomedica Article INTRODUCTION: It is essential to determine the interactions between viruses and mosquitoes to diminish dengue viral transmission. These interactions constitute a very complex system of highly regulated pathways known as the innate immune system of the mosquito, which produces antimicrobial peptides that act as effector molecules against bacterial and fungal infections. There is less information about such effects on virus infections. OBJECTIVE: To determine the expression of two antimicrobial peptide genes, defensin A and cecropin A, in Aedes aegypti mosquitoes infected with DENV-1. MATERIALS AND METHODS: We used the F(1) generation of mosquitoes orally infected with DENV-1 and real-time PCR analysis to determine whether the defensin A and cecropin A genes played a role in controlling DENV-1 replication in Ae. aegypti. As a reference, we conducted similar experiments with the bacteria Escherichia coli. RESULTS: Basal levels of defensin A and cecropin A mRNA were expressed in uninfected mosquitoes at different times post-blood feeding. The infected mosquitoes experienced reduced expression of these mRNA by at least eightfold when compared to uninfected control mosquitoes at all times post-infection. In contrast with the behavior of DENV-1, results showed that bacterial infection produced up-regulation of defensin and cecropin genes; however, the induction of transcripts occurred at later times (15 days). Conclusion: DENV-1 virus inhibited the expression of defensin A and cecropin A genes in a wild Ae. aegypti population from Venezuela. Biteca 2020-08-28 /pmc/articles/PMC8055593/ /pubmed/33761199 http://dx.doi.org/10.7705/biomedica.5491 Text en https://creativecommons.org/licenses/by/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Méndez, Yda Pacheco, César Herrera, Flor Inhibition of defensin A and cecropin A responses to dengue virus 1 infection in Aedes aegypti |
title | Inhibition of defensin A and cecropin A responses to dengue virus 1 infection in Aedes aegypti |
title_full | Inhibition of defensin A and cecropin A responses to dengue virus 1 infection in Aedes aegypti |
title_fullStr | Inhibition of defensin A and cecropin A responses to dengue virus 1 infection in Aedes aegypti |
title_full_unstemmed | Inhibition of defensin A and cecropin A responses to dengue virus 1 infection in Aedes aegypti |
title_short | Inhibition of defensin A and cecropin A responses to dengue virus 1 infection in Aedes aegypti |
title_sort | inhibition of defensin a and cecropin a responses to dengue virus 1 infection in aedes aegypti |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055593/ https://www.ncbi.nlm.nih.gov/pubmed/33761199 http://dx.doi.org/10.7705/biomedica.5491 |
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