Pooled CRISPR-activation screening coupled with single-cell RNA-seq in mouse embryonic stem cells

CRISPR/Cas9 screens are a powerful approach to identify key regulators of biological processes. By combining pooled CRISPR/Cas9 screening with single-cell RNA-sequencing readout, individual perturbations can be assessed in parallel both comprehensively and at scale. Importantly, this allows gene fun...

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Detalles Bibliográficos
Autores principales: Alda-Catalinas, Celia, Eckersley-Maslin, Melanie A., Reik, Wolf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055612/
https://www.ncbi.nlm.nih.gov/pubmed/33899013
http://dx.doi.org/10.1016/j.xpro.2021.100426

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