Modeling retinitis pigmentosa through patient-derived retinal organoids

Human-induced pluripotent stem cells (hiPSCs) can be differentiated into well-structured retinal organoids. In this protocol, we successfully established 3D retinae from patient-derived hiPSCs and built the retinitis pigmentosa model in vitro. Moreover, mutation in the retinitis pigmentosa GTPase re...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Yan-Ping, Deng, Wen-Li, Jin, Zi-Bing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055708/
https://www.ncbi.nlm.nih.gov/pubmed/33899019
http://dx.doi.org/10.1016/j.xpro.2021.100438
_version_ 1783680507838464000
author Li, Yan-Ping
Deng, Wen-Li
Jin, Zi-Bing
author_facet Li, Yan-Ping
Deng, Wen-Li
Jin, Zi-Bing
author_sort Li, Yan-Ping
collection PubMed
description Human-induced pluripotent stem cells (hiPSCs) can be differentiated into well-structured retinal organoids. In this protocol, we successfully established 3D retinae from patient-derived hiPSCs and built the retinitis pigmentosa model in vitro. Moreover, mutation in the retinitis pigmentosa GTPase regulator (RPGR) gene was corrected by CRISPR-Cas9 gene editing, which rescued the structure and function of the 3D retinae. For complete details on the use and execution of this protocol, please refer to Deng et al. (2018).
format Online
Article
Text
id pubmed-8055708
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-80557082021-04-23 Modeling retinitis pigmentosa through patient-derived retinal organoids Li, Yan-Ping Deng, Wen-Li Jin, Zi-Bing STAR Protoc Protocol Human-induced pluripotent stem cells (hiPSCs) can be differentiated into well-structured retinal organoids. In this protocol, we successfully established 3D retinae from patient-derived hiPSCs and built the retinitis pigmentosa model in vitro. Moreover, mutation in the retinitis pigmentosa GTPase regulator (RPGR) gene was corrected by CRISPR-Cas9 gene editing, which rescued the structure and function of the 3D retinae. For complete details on the use and execution of this protocol, please refer to Deng et al. (2018). Elsevier 2021-04-08 /pmc/articles/PMC8055708/ /pubmed/33899019 http://dx.doi.org/10.1016/j.xpro.2021.100438 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Li, Yan-Ping
Deng, Wen-Li
Jin, Zi-Bing
Modeling retinitis pigmentosa through patient-derived retinal organoids
title Modeling retinitis pigmentosa through patient-derived retinal organoids
title_full Modeling retinitis pigmentosa through patient-derived retinal organoids
title_fullStr Modeling retinitis pigmentosa through patient-derived retinal organoids
title_full_unstemmed Modeling retinitis pigmentosa through patient-derived retinal organoids
title_short Modeling retinitis pigmentosa through patient-derived retinal organoids
title_sort modeling retinitis pigmentosa through patient-derived retinal organoids
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055708/
https://www.ncbi.nlm.nih.gov/pubmed/33899019
http://dx.doi.org/10.1016/j.xpro.2021.100438
work_keys_str_mv AT liyanping modelingretinitispigmentosathroughpatientderivedretinalorganoids
AT dengwenli modelingretinitispigmentosathroughpatientderivedretinalorganoids
AT jinzibing modelingretinitispigmentosathroughpatientderivedretinalorganoids

Ejemplares similares