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Characterization of Strip1 Expression in Mouse Cochlear Hair Cells

Striatin-interacting protein 1 (Strip1) is a core component of the striatin interacting phosphatase and kinase (STRIPAK) complex, which is involved in embryogenesis and development, circadian rhythms, type 2 diabetes, and cancer progression. However, the expression and role of Strip1 in the mammalia...

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Autores principales: Zhang, Shasha, Dong, Ying, Qiang, Ruiying, Zhang, Yuan, Zhang, Xiaoli, Chen, Yin, Jiang, Pei, Ma, Xiangyu, Wu, Leilei, Ai, Jingru, Gao, Xia, Wang, Pengjun, Chen, Jie, Chai, Renjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056008/
https://www.ncbi.nlm.nih.gov/pubmed/33889175
http://dx.doi.org/10.3389/fgene.2021.625867
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author Zhang, Shasha
Dong, Ying
Qiang, Ruiying
Zhang, Yuan
Zhang, Xiaoli
Chen, Yin
Jiang, Pei
Ma, Xiangyu
Wu, Leilei
Ai, Jingru
Gao, Xia
Wang, Pengjun
Chen, Jie
Chai, Renjie
author_facet Zhang, Shasha
Dong, Ying
Qiang, Ruiying
Zhang, Yuan
Zhang, Xiaoli
Chen, Yin
Jiang, Pei
Ma, Xiangyu
Wu, Leilei
Ai, Jingru
Gao, Xia
Wang, Pengjun
Chen, Jie
Chai, Renjie
author_sort Zhang, Shasha
collection PubMed
description Striatin-interacting protein 1 (Strip1) is a core component of the striatin interacting phosphatase and kinase (STRIPAK) complex, which is involved in embryogenesis and development, circadian rhythms, type 2 diabetes, and cancer progression. However, the expression and role of Strip1 in the mammalian cochlea remains unclear. Here we studied the expression and function of Strip1 in the mouse cochlea by using Strip1 knockout mice. We first found that the mRNA and protein expression of Strip1 increases as mice age starting from postnatal day (P) 3 and reaches its highest expression level at P30 and that the expression of Strip1 can be detected by immunofluorescent staining starting from P14 only in cochlear HCs, and not in supporting cells (SCs). Next, we crossed Strip1 heterozygous knockout (Strip +/−) mice to obtain Strip1 homozygous knockout (Strip1−/−) mice for studying the role of Strip1 in cochlear HCs. However, no Strip1−/− mice were obtained and the ratio of Strip +/− to Strip1+/+ mice per litter was about 2:1, which suggested that homozygous Strip1 knockout is embryonic lethal. We measured hearing function and counted the HC number in P30 and P60 Strip +/− mice and found that they had normal hearing ability and HC numbers compared to Strip1+/+ mice. Our study suggested that Strip1 probably play important roles in HC development and maturation, which needs further study in the future.
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spelling pubmed-80560082021-04-21 Characterization of Strip1 Expression in Mouse Cochlear Hair Cells Zhang, Shasha Dong, Ying Qiang, Ruiying Zhang, Yuan Zhang, Xiaoli Chen, Yin Jiang, Pei Ma, Xiangyu Wu, Leilei Ai, Jingru Gao, Xia Wang, Pengjun Chen, Jie Chai, Renjie Front Genet Genetics Striatin-interacting protein 1 (Strip1) is a core component of the striatin interacting phosphatase and kinase (STRIPAK) complex, which is involved in embryogenesis and development, circadian rhythms, type 2 diabetes, and cancer progression. However, the expression and role of Strip1 in the mammalian cochlea remains unclear. Here we studied the expression and function of Strip1 in the mouse cochlea by using Strip1 knockout mice. We first found that the mRNA and protein expression of Strip1 increases as mice age starting from postnatal day (P) 3 and reaches its highest expression level at P30 and that the expression of Strip1 can be detected by immunofluorescent staining starting from P14 only in cochlear HCs, and not in supporting cells (SCs). Next, we crossed Strip1 heterozygous knockout (Strip +/−) mice to obtain Strip1 homozygous knockout (Strip1−/−) mice for studying the role of Strip1 in cochlear HCs. However, no Strip1−/− mice were obtained and the ratio of Strip +/− to Strip1+/+ mice per litter was about 2:1, which suggested that homozygous Strip1 knockout is embryonic lethal. We measured hearing function and counted the HC number in P30 and P60 Strip +/− mice and found that they had normal hearing ability and HC numbers compared to Strip1+/+ mice. Our study suggested that Strip1 probably play important roles in HC development and maturation, which needs further study in the future. Frontiers Media S.A. 2021-04-06 /pmc/articles/PMC8056008/ /pubmed/33889175 http://dx.doi.org/10.3389/fgene.2021.625867 Text en Copyright © 2021 Zhang, Dong, Qiang, Zhang, Zhang, Chen, Jiang, Ma, Wu, Ai, Gao, Wang, Chen and Chai. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Zhang, Shasha
Dong, Ying
Qiang, Ruiying
Zhang, Yuan
Zhang, Xiaoli
Chen, Yin
Jiang, Pei
Ma, Xiangyu
Wu, Leilei
Ai, Jingru
Gao, Xia
Wang, Pengjun
Chen, Jie
Chai, Renjie
Characterization of Strip1 Expression in Mouse Cochlear Hair Cells
title Characterization of Strip1 Expression in Mouse Cochlear Hair Cells
title_full Characterization of Strip1 Expression in Mouse Cochlear Hair Cells
title_fullStr Characterization of Strip1 Expression in Mouse Cochlear Hair Cells
title_full_unstemmed Characterization of Strip1 Expression in Mouse Cochlear Hair Cells
title_short Characterization of Strip1 Expression in Mouse Cochlear Hair Cells
title_sort characterization of strip1 expression in mouse cochlear hair cells
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056008/
https://www.ncbi.nlm.nih.gov/pubmed/33889175
http://dx.doi.org/10.3389/fgene.2021.625867
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