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QTL Mapping and Favorable Allele Mining of Nitrogen Deficiency Tolerance Using an Interconnected Breeding Population in Rice

Nitrogen is one of the most important nutrients for rice growth and development. Breeding of nitrogen deficiency tolerance (NDT) variety is considered to be the most economic measure to solve the constrain of low nitrogen stress on grain yield in rice. An interconnected breeding (IB) population of 4...

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Autores principales: Shen, Congcong, Chen, Kai, Cui, Yanru, Chen, Jiantao, Mi, Xuefei, Zhu, Shuangbin, Zhu, Yajun, Ali, Jauhar, Ye, Guoyou, Li, Zhikang, Xu, Jianlong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056011/
https://www.ncbi.nlm.nih.gov/pubmed/33889173
http://dx.doi.org/10.3389/fgene.2021.616428
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author Shen, Congcong
Chen, Kai
Cui, Yanru
Chen, Jiantao
Mi, Xuefei
Zhu, Shuangbin
Zhu, Yajun
Ali, Jauhar
Ye, Guoyou
Li, Zhikang
Xu, Jianlong
author_facet Shen, Congcong
Chen, Kai
Cui, Yanru
Chen, Jiantao
Mi, Xuefei
Zhu, Shuangbin
Zhu, Yajun
Ali, Jauhar
Ye, Guoyou
Li, Zhikang
Xu, Jianlong
author_sort Shen, Congcong
collection PubMed
description Nitrogen is one of the most important nutrients for rice growth and development. Breeding of nitrogen deficiency tolerance (NDT) variety is considered to be the most economic measure to solve the constrain of low nitrogen stress on grain yield in rice. An interconnected breeding (IB) population of 497 lines developed using Huanghuazhan (HHZ) as the recurrent parent and eight elite lines as the donor parents were tested for five traits including grain yield, biomass, harvest index, thousand grain weight, and spikelet fertility under two nitrogen treatments in three growing seasons. Association analysis using 7,388 bins generated by sequencing identified a total of 14, 14, and 12 QTLs for the five traits under low nitrogen (LN), normal nitrogen (NN), and LN/NN conditions, respectively, across three seasons. Favorable alleles were dissected for the 40 QTLs at the 10 NDT regions, and OM1723 was considered as the most important parent with the highest frequency of favorable alleles contributing to NDT-related traits. Six superior lines all showed significantly higher GY in LN environments and similar GY under NN environments except for H10. Substitution mapping using near-isogenic introgression lines delimited the qTGW2-1, which was identified on chromosome 2 under LN, NN, and LN/NN conditions into two QTLs, which were located in the two regions of about 200 and 350 kb with different favorable alleles. The bins 16, 1301, 1465, 1486, 3464, and 6249 harbored the QTLs for NDT detected in this study, and the QTLs/genes previously identified for NDT or nitrogen use efficiency (NUE) could be used for enhancing NDT and NUE by marker-assisted selection (MAS).
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spelling pubmed-80560112021-04-21 QTL Mapping and Favorable Allele Mining of Nitrogen Deficiency Tolerance Using an Interconnected Breeding Population in Rice Shen, Congcong Chen, Kai Cui, Yanru Chen, Jiantao Mi, Xuefei Zhu, Shuangbin Zhu, Yajun Ali, Jauhar Ye, Guoyou Li, Zhikang Xu, Jianlong Front Genet Genetics Nitrogen is one of the most important nutrients for rice growth and development. Breeding of nitrogen deficiency tolerance (NDT) variety is considered to be the most economic measure to solve the constrain of low nitrogen stress on grain yield in rice. An interconnected breeding (IB) population of 497 lines developed using Huanghuazhan (HHZ) as the recurrent parent and eight elite lines as the donor parents were tested for five traits including grain yield, biomass, harvest index, thousand grain weight, and spikelet fertility under two nitrogen treatments in three growing seasons. Association analysis using 7,388 bins generated by sequencing identified a total of 14, 14, and 12 QTLs for the five traits under low nitrogen (LN), normal nitrogen (NN), and LN/NN conditions, respectively, across three seasons. Favorable alleles were dissected for the 40 QTLs at the 10 NDT regions, and OM1723 was considered as the most important parent with the highest frequency of favorable alleles contributing to NDT-related traits. Six superior lines all showed significantly higher GY in LN environments and similar GY under NN environments except for H10. Substitution mapping using near-isogenic introgression lines delimited the qTGW2-1, which was identified on chromosome 2 under LN, NN, and LN/NN conditions into two QTLs, which were located in the two regions of about 200 and 350 kb with different favorable alleles. The bins 16, 1301, 1465, 1486, 3464, and 6249 harbored the QTLs for NDT detected in this study, and the QTLs/genes previously identified for NDT or nitrogen use efficiency (NUE) could be used for enhancing NDT and NUE by marker-assisted selection (MAS). Frontiers Media S.A. 2021-04-06 /pmc/articles/PMC8056011/ /pubmed/33889173 http://dx.doi.org/10.3389/fgene.2021.616428 Text en Copyright © 2021 Shen, Chen, Cui, Chen, Mi, Zhu, Zhu, Ali, Ye, Li and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Shen, Congcong
Chen, Kai
Cui, Yanru
Chen, Jiantao
Mi, Xuefei
Zhu, Shuangbin
Zhu, Yajun
Ali, Jauhar
Ye, Guoyou
Li, Zhikang
Xu, Jianlong
QTL Mapping and Favorable Allele Mining of Nitrogen Deficiency Tolerance Using an Interconnected Breeding Population in Rice
title QTL Mapping and Favorable Allele Mining of Nitrogen Deficiency Tolerance Using an Interconnected Breeding Population in Rice
title_full QTL Mapping and Favorable Allele Mining of Nitrogen Deficiency Tolerance Using an Interconnected Breeding Population in Rice
title_fullStr QTL Mapping and Favorable Allele Mining of Nitrogen Deficiency Tolerance Using an Interconnected Breeding Population in Rice
title_full_unstemmed QTL Mapping and Favorable Allele Mining of Nitrogen Deficiency Tolerance Using an Interconnected Breeding Population in Rice
title_short QTL Mapping and Favorable Allele Mining of Nitrogen Deficiency Tolerance Using an Interconnected Breeding Population in Rice
title_sort qtl mapping and favorable allele mining of nitrogen deficiency tolerance using an interconnected breeding population in rice
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056011/
https://www.ncbi.nlm.nih.gov/pubmed/33889173
http://dx.doi.org/10.3389/fgene.2021.616428
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