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Evaluation of the cytotoxic and immunogenic potential of temozolamide, panobinostat, and Lophophora williamsii extract against C6 glioma cells
Glioblastoma multiforme is a malignant neoplasm of the brain with poor prognosis. The first-line drug against glioblastoma is the alkylating agent temozolamide (TMZ); unfortunately, treatment resistance and tumor re-incidence are common. In some cases, immunogenic cell death (ICD) inducers can decre...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Leibniz Research Centre for Working Environment and Human Factors
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056056/ https://www.ncbi.nlm.nih.gov/pubmed/33883986 http://dx.doi.org/10.17179/excli2020-3181 |
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author | Franco-Molina, Moisés Armides Santana-Krímskaya, Silvia Elena Madrigal-de-León, Luis Mario Coronado-Cerda, Erika Evangelina Zárate-Triviño, Diana Ginette Hernández-Martínez, Sara Paola García-Coronado, Paola Leonor Rodríguez-Padilla, Cristina |
author_facet | Franco-Molina, Moisés Armides Santana-Krímskaya, Silvia Elena Madrigal-de-León, Luis Mario Coronado-Cerda, Erika Evangelina Zárate-Triviño, Diana Ginette Hernández-Martínez, Sara Paola García-Coronado, Paola Leonor Rodríguez-Padilla, Cristina |
author_sort | Franco-Molina, Moisés Armides |
collection | PubMed |
description | Glioblastoma multiforme is a malignant neoplasm of the brain with poor prognosis. The first-line drug against glioblastoma is the alkylating agent temozolamide (TMZ); unfortunately, treatment resistance and tumor re-incidence are common. In some cases, immunogenic cell death (ICD) inducers can decrease treatment resistance and tumor recurrence by stimulating an antitumor specific immune response. Not all ICD inducers, however, are suitable for glioma patients because of the low permeability of the blood-brain barrier (BBB). Panobinostat (PAN), a histone deacetylase inhibitor and Lophophora williamsii (LW) extract can pass through the BBB and have antitumor properties. The aim of this study is to evaluate the cytotoxic potential of TMZ, PAN and LW extract against the glioma C6 cell line, and its role in the release of damage-associated molecular patterns (DAMPs), which is a hallmark of ICD. Our results indicate that all treatments induce cellular death in a time- and concentration-dependent manner, and that PAN and LW extract induce apoptosis, whereas TMZ induces apoptosis and necrosis. Also, that some of the treatments and their sequential administration induce the release of DAMPs. Furthermore, in a rat glioma model, we observed that all treatments decreased tumor volume, but the in vivo cell death mechanism was not ICD. Our findings indicate that TMZ, PAN, and LW combination have a cytotoxic effect against glioma cells but do not induce ICD. |
format | Online Article Text |
id | pubmed-8056056 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Leibniz Research Centre for Working Environment and Human Factors |
record_format | MEDLINE/PubMed |
spelling | pubmed-80560562021-04-20 Evaluation of the cytotoxic and immunogenic potential of temozolamide, panobinostat, and Lophophora williamsii extract against C6 glioma cells Franco-Molina, Moisés Armides Santana-Krímskaya, Silvia Elena Madrigal-de-León, Luis Mario Coronado-Cerda, Erika Evangelina Zárate-Triviño, Diana Ginette Hernández-Martínez, Sara Paola García-Coronado, Paola Leonor Rodríguez-Padilla, Cristina EXCLI J Original Article Glioblastoma multiforme is a malignant neoplasm of the brain with poor prognosis. The first-line drug against glioblastoma is the alkylating agent temozolamide (TMZ); unfortunately, treatment resistance and tumor re-incidence are common. In some cases, immunogenic cell death (ICD) inducers can decrease treatment resistance and tumor recurrence by stimulating an antitumor specific immune response. Not all ICD inducers, however, are suitable for glioma patients because of the low permeability of the blood-brain barrier (BBB). Panobinostat (PAN), a histone deacetylase inhibitor and Lophophora williamsii (LW) extract can pass through the BBB and have antitumor properties. The aim of this study is to evaluate the cytotoxic potential of TMZ, PAN and LW extract against the glioma C6 cell line, and its role in the release of damage-associated molecular patterns (DAMPs), which is a hallmark of ICD. Our results indicate that all treatments induce cellular death in a time- and concentration-dependent manner, and that PAN and LW extract induce apoptosis, whereas TMZ induces apoptosis and necrosis. Also, that some of the treatments and their sequential administration induce the release of DAMPs. Furthermore, in a rat glioma model, we observed that all treatments decreased tumor volume, but the in vivo cell death mechanism was not ICD. Our findings indicate that TMZ, PAN, and LW combination have a cytotoxic effect against glioma cells but do not induce ICD. Leibniz Research Centre for Working Environment and Human Factors 2021-03-09 /pmc/articles/PMC8056056/ /pubmed/33883986 http://dx.doi.org/10.17179/excli2020-3181 Text en Copyright © 2021 Franco-Molina et al. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ) You are free to copy, distribute and transmit the work, provided the original author and source are credited. |
spellingShingle | Original Article Franco-Molina, Moisés Armides Santana-Krímskaya, Silvia Elena Madrigal-de-León, Luis Mario Coronado-Cerda, Erika Evangelina Zárate-Triviño, Diana Ginette Hernández-Martínez, Sara Paola García-Coronado, Paola Leonor Rodríguez-Padilla, Cristina Evaluation of the cytotoxic and immunogenic potential of temozolamide, panobinostat, and Lophophora williamsii extract against C6 glioma cells |
title | Evaluation of the cytotoxic and immunogenic potential of temozolamide, panobinostat, and Lophophora williamsii extract against C6 glioma cells |
title_full | Evaluation of the cytotoxic and immunogenic potential of temozolamide, panobinostat, and Lophophora williamsii extract against C6 glioma cells |
title_fullStr | Evaluation of the cytotoxic and immunogenic potential of temozolamide, panobinostat, and Lophophora williamsii extract against C6 glioma cells |
title_full_unstemmed | Evaluation of the cytotoxic and immunogenic potential of temozolamide, panobinostat, and Lophophora williamsii extract against C6 glioma cells |
title_short | Evaluation of the cytotoxic and immunogenic potential of temozolamide, panobinostat, and Lophophora williamsii extract against C6 glioma cells |
title_sort | evaluation of the cytotoxic and immunogenic potential of temozolamide, panobinostat, and lophophora williamsii extract against c6 glioma cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056056/ https://www.ncbi.nlm.nih.gov/pubmed/33883986 http://dx.doi.org/10.17179/excli2020-3181 |
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