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E2F2 stimulates CCR4 expression and activates synovial fibroblast-like cells in rheumatoid arthritis
AIM OF THE STUDY: E2F transcription factor 2 (E2F2) has increased expression in synovial tissues of rheumatoid arthritis (RA) and stimulates interleukin (IL)-1 α and IL-β production in cultured RA synovial fibroblast-like cells (RASF), which supports the importance of E2F2 in RA pathogenesis. This s...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Termedia Publishing House
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056345/ https://www.ncbi.nlm.nih.gov/pubmed/33897281 http://dx.doi.org/10.5114/ceji.2021.105243 |
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author | Xu, Wanju Li, Shufeng Chang, Xiaotian |
author_facet | Xu, Wanju Li, Shufeng Chang, Xiaotian |
author_sort | Xu, Wanju |
collection | PubMed |
description | AIM OF THE STUDY: E2F transcription factor 2 (E2F2) has increased expression in synovial tissues of rheumatoid arthritis (RA) and stimulates interleukin (IL)-1 α and IL-β production in cultured RA synovial fibroblast-like cells (RASF), which supports the importance of E2F2 in RA pathogenesis. This study investigated the effect and mechanism of E2F2 in RA. MATERIAL AND METHODS: Cultured RASF were transfected with anti-E2F2 siRNA, and the expression profile was analyzed with an inflammatory response and autoimmunity PCR array loaded with 84-relative genes to explore the pathogenic pathway of E2F2. Apoptosis, migration and tube-like structure formation in the RASF with transfection of anti-E2F2 siRNA or E2F2-expressing plasmids were examined using flow cytometry, transwell assays and Matrigel assays, respectively. RESULTS: Significantly decreased expression of chemokine receptor 4 (CCR4) was detected in RASF with inhibited E2F2 expression, and the CCR4 expression was increased in RASF with transfection of E2F2-expressing plasmids. Silencing E2F2 expression stimulated apoptosis, but retarded migration and tube-like structure formation in RASF. The opposite observation was obtained in RASF with E2F2 overexpression. CONCLUSIONS: High E2F2 expression decreases apoptosis and increases migration and tube-like structure ability in RASF and might perform this role by up-regulating CCR4 expression, which ultimately contributes to the disease progression of RA synovial tissues. |
format | Online Article Text |
id | pubmed-8056345 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Termedia Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-80563452021-04-23 E2F2 stimulates CCR4 expression and activates synovial fibroblast-like cells in rheumatoid arthritis Xu, Wanju Li, Shufeng Chang, Xiaotian Cent Eur J Immunol Experimental Immunology AIM OF THE STUDY: E2F transcription factor 2 (E2F2) has increased expression in synovial tissues of rheumatoid arthritis (RA) and stimulates interleukin (IL)-1 α and IL-β production in cultured RA synovial fibroblast-like cells (RASF), which supports the importance of E2F2 in RA pathogenesis. This study investigated the effect and mechanism of E2F2 in RA. MATERIAL AND METHODS: Cultured RASF were transfected with anti-E2F2 siRNA, and the expression profile was analyzed with an inflammatory response and autoimmunity PCR array loaded with 84-relative genes to explore the pathogenic pathway of E2F2. Apoptosis, migration and tube-like structure formation in the RASF with transfection of anti-E2F2 siRNA or E2F2-expressing plasmids were examined using flow cytometry, transwell assays and Matrigel assays, respectively. RESULTS: Significantly decreased expression of chemokine receptor 4 (CCR4) was detected in RASF with inhibited E2F2 expression, and the CCR4 expression was increased in RASF with transfection of E2F2-expressing plasmids. Silencing E2F2 expression stimulated apoptosis, but retarded migration and tube-like structure formation in RASF. The opposite observation was obtained in RASF with E2F2 overexpression. CONCLUSIONS: High E2F2 expression decreases apoptosis and increases migration and tube-like structure ability in RASF and might perform this role by up-regulating CCR4 expression, which ultimately contributes to the disease progression of RA synovial tissues. Termedia Publishing House 2021-04-18 2021 /pmc/articles/PMC8056345/ /pubmed/33897281 http://dx.doi.org/10.5114/ceji.2021.105243 Text en Copyright © 2021 Termedia https://creativecommons.org/licenses/by-nc-sa/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0). License (http://creativecommons.org/licenses/by-nc-sa/4.0/ (https://creativecommons.org/licenses/by-nc-sa/4.0/) ) |
spellingShingle | Experimental Immunology Xu, Wanju Li, Shufeng Chang, Xiaotian E2F2 stimulates CCR4 expression and activates synovial fibroblast-like cells in rheumatoid arthritis |
title | E2F2 stimulates CCR4 expression and activates synovial fibroblast-like cells in rheumatoid arthritis |
title_full | E2F2 stimulates CCR4 expression and activates synovial fibroblast-like cells in rheumatoid arthritis |
title_fullStr | E2F2 stimulates CCR4 expression and activates synovial fibroblast-like cells in rheumatoid arthritis |
title_full_unstemmed | E2F2 stimulates CCR4 expression and activates synovial fibroblast-like cells in rheumatoid arthritis |
title_short | E2F2 stimulates CCR4 expression and activates synovial fibroblast-like cells in rheumatoid arthritis |
title_sort | e2f2 stimulates ccr4 expression and activates synovial fibroblast-like cells in rheumatoid arthritis |
topic | Experimental Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056345/ https://www.ncbi.nlm.nih.gov/pubmed/33897281 http://dx.doi.org/10.5114/ceji.2021.105243 |
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