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Enhanced Azidolysis by the Formation of Stable Ser–His Catalytic Dyad in a Glycoside Hydrolase Family 10 Xylanase Mutant
Glycoside hydrolases require carboxyl groups as catalysts for their activity. A retaining xylanase from Streptomyces olivaceoviridis E-86 belonging to glycoside hydrolase family 10 possesses Glu128 and Glu236 that respectively function as acid/base and nucleophile. We previously developed a unique m...
Autores principales: | Suzuki, Ryuichiro, Fujimoto, Zui, Kaneko, Satoshi, Hasegawa, Tsunemi, Kuno, Atsushi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Applied Glycoscience
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056907/ https://www.ncbi.nlm.nih.gov/pubmed/34354506 http://dx.doi.org/10.5458/jag.jag.JAG-2017_011 |
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