Construction of Cellulose Binding Domain Fusion FMN-Dependent NADH-Azoreductase and Glucose 1-Dehydrogenase for the Development of Flow Injection Analysis with Fusion Enzymes Immobilized on Cellulose

The cellulose binding domain (CBD) of cellulosome-integrating protein A from Clostridium thermocellum NBRC 103400 was genetically fused to FMN-dependent NADH-azoreductase (AZR) and glucose 1-dehydrogenase (GDH) from Bacillus subtilis. The fusion enzymes, AZR-CBD and CBD-GDH, were expressed in Escher...

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Detalles Bibliográficos
Autores principales: Yano, Shigekazu, Hori, Yukari, Kijima, Tatsuro, Konno, Hiroyuki, Suyotha, Wasana, Takagi, Kazuyoshi, Wakayama, Mamoru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Applied Glycoscience 2019
Materias:
Regular Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056932/
https://www.ncbi.nlm.nih.gov/pubmed/34354522
http://dx.doi.org/10.5458/jag.jag.JAG-2018_0011

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