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Production of Gentiobiose from Hydrothermally Treated Aureobasidium pullulans β-1,3-1,6-Glucan
We report production of the functional disaccharide gentiobiose β-D-Glcp-(1→6)-D-Glc by a hydrolysis reaction of hydrothermally treated Aureobasidium pullulans β-1,3-1,6-glucan as the substrate and Kitalase as the enzyme. Gentiobiose was produced over the pH range 4−6 and the concentration of gentio...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Applied Glycoscience
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056935/ https://www.ncbi.nlm.nih.gov/pubmed/34354494 http://dx.doi.org/10.5458/jag.jag.JAG-2017_002 |
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author | Hirabayashi, Katsuki Tashiro, Yoshiya Kondo, Nobuhiro Hayashi, Sachio |
author_facet | Hirabayashi, Katsuki Tashiro, Yoshiya Kondo, Nobuhiro Hayashi, Sachio |
author_sort | Hirabayashi, Katsuki |
collection | PubMed |
description | We report production of the functional disaccharide gentiobiose β-D-Glcp-(1→6)-D-Glc by a hydrolysis reaction of hydrothermally treated Aureobasidium pullulans β-1,3-1,6-glucan as the substrate and Kitalase as the enzyme. Gentiobiose was produced over the pH range 4−6 and the concentration of gentiobiose produced decreased above pH 7. The maximum value of gentiobiose production was unaffected by the enzyme concentration. The maximum concentration of gentiobiose produced was dependent on the substrate concentration whereas the maximum ratio of gentiobiose to glucose was not. The production of gentiobiose from yeast β-1,3-1,6-glucan was lower than that from A. pullulans β-1,3-1,6-glucan. |
format | Online Article Text |
id | pubmed-8056935 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Japanese Society of Applied Glycoscience |
record_format | MEDLINE/PubMed |
spelling | pubmed-80569352021-08-04 Production of Gentiobiose from Hydrothermally Treated Aureobasidium pullulans β-1,3-1,6-Glucan Hirabayashi, Katsuki Tashiro, Yoshiya Kondo, Nobuhiro Hayashi, Sachio J Appl Glycosci (1999) Regular Paper We report production of the functional disaccharide gentiobiose β-D-Glcp-(1→6)-D-Glc by a hydrolysis reaction of hydrothermally treated Aureobasidium pullulans β-1,3-1,6-glucan as the substrate and Kitalase as the enzyme. Gentiobiose was produced over the pH range 4−6 and the concentration of gentiobiose produced decreased above pH 7. The maximum value of gentiobiose production was unaffected by the enzyme concentration. The maximum concentration of gentiobiose produced was dependent on the substrate concentration whereas the maximum ratio of gentiobiose to glucose was not. The production of gentiobiose from yeast β-1,3-1,6-glucan was lower than that from A. pullulans β-1,3-1,6-glucan. The Japanese Society of Applied Glycoscience 2017-05-20 /pmc/articles/PMC8056935/ /pubmed/34354494 http://dx.doi.org/10.5458/jag.jag.JAG-2017_002 Text en 2017 by The Japanese Society of Applied Glycoscience https://creativecommons.org/licenses/by-nc/4.0/This is an open-access paper distributed under the terms of the Creative Commons Attribution Non-Commercial (by-nc) License (CC-BY-NC4.0: https://creativecommons.org/licenses/by-nc/4.0/). |
spellingShingle | Regular Paper Hirabayashi, Katsuki Tashiro, Yoshiya Kondo, Nobuhiro Hayashi, Sachio Production of Gentiobiose from Hydrothermally Treated Aureobasidium pullulans β-1,3-1,6-Glucan |
title | Production of Gentiobiose from Hydrothermally Treated Aureobasidium pullulans β-1,3-1,6-Glucan |
title_full | Production of Gentiobiose from Hydrothermally Treated Aureobasidium pullulans β-1,3-1,6-Glucan |
title_fullStr | Production of Gentiobiose from Hydrothermally Treated Aureobasidium pullulans β-1,3-1,6-Glucan |
title_full_unstemmed | Production of Gentiobiose from Hydrothermally Treated Aureobasidium pullulans β-1,3-1,6-Glucan |
title_short | Production of Gentiobiose from Hydrothermally Treated Aureobasidium pullulans β-1,3-1,6-Glucan |
title_sort | production of gentiobiose from hydrothermally treated aureobasidium pullulans β-1,3-1,6-glucan |
topic | Regular Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056935/ https://www.ncbi.nlm.nih.gov/pubmed/34354494 http://dx.doi.org/10.5458/jag.jag.JAG-2017_002 |
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