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The effects of flavonoids, green tea polyphenols and coffee on DMBA induced LINE-1 DNA hypomethylation

The intake of carcinogenic and chemopreventive compounds are important nutritional factors related to the development of malignant tumorous diseases. Repetitive long interspersed element-1 (LINE-1) DNA methylation pattern plays a key role in both carcinogenesis and chemoprevention. In our present in...

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Autores principales: Szabo, Laszlo, Molnar, Richard, Tomesz, Andras, Deutsch, Arpad, Darago, Richard, Nowrasteh, Ghodratollah, Varjas, Timea, Nemeth, Balazs, Budan, Ferenc, Kiss, Istvan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8057585/
https://www.ncbi.nlm.nih.gov/pubmed/33878138
http://dx.doi.org/10.1371/journal.pone.0250157
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author Szabo, Laszlo
Molnar, Richard
Tomesz, Andras
Deutsch, Arpad
Darago, Richard
Nowrasteh, Ghodratollah
Varjas, Timea
Nemeth, Balazs
Budan, Ferenc
Kiss, Istvan
author_facet Szabo, Laszlo
Molnar, Richard
Tomesz, Andras
Deutsch, Arpad
Darago, Richard
Nowrasteh, Ghodratollah
Varjas, Timea
Nemeth, Balazs
Budan, Ferenc
Kiss, Istvan
author_sort Szabo, Laszlo
collection PubMed
description The intake of carcinogenic and chemopreventive compounds are important nutritional factors related to the development of malignant tumorous diseases. Repetitive long interspersed element-1 (LINE-1) DNA methylation pattern plays a key role in both carcinogenesis and chemoprevention. In our present in vivo animal model, we examined LINE-1 DNA methylation pattern as potential biomarker in the liver, spleen and kidney of mice consuming green tea (Camellia sinensis) extract (catechins 80%), a chinese bayberry (Morella rubra) extract (myricetin 80%), a flavonoid extract (with added resveratrol) and coffee (Coffee arabica) extract. In the organs examined, carcinogen 7,12-dimethylbenz(a)anthracene (DMBA)-induced hypomethylation was prevented by all test materials except chinese bayberry extract in the kidneys. Moreover, the flavonoid extract caused significant hypermethylation in the liver compared to untreated controls and to other test materials. The tested chemopreventive substances have antioxidant, anti-inflammatory properties and regulate molecular biological signaling pathways. They increase glutathione levels, induce antioxidant enzymes, which decrease free radical damage caused by DMBA, and ultimately, they are able to increase the activity of DNA methyltransferase enzymes. Furthermore, flavonoids in the liver may inhibit the procarcinogen to carcinogen activation of DMBA through the inhibition of CYP1A1 enzyme. At the same time, paradoxically, myricetin can act as a prooxidant as a result of free radical damage, which can explain that it did not prevent hypomethylation in the kidneys. Our results demonstrated that LINE-1 DNA methylation pattern is a useful potential biomarker for detecting and monitoring carcinogenic and chemopreventive effects of dietary compounds.
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spelling pubmed-80575852021-05-04 The effects of flavonoids, green tea polyphenols and coffee on DMBA induced LINE-1 DNA hypomethylation Szabo, Laszlo Molnar, Richard Tomesz, Andras Deutsch, Arpad Darago, Richard Nowrasteh, Ghodratollah Varjas, Timea Nemeth, Balazs Budan, Ferenc Kiss, Istvan PLoS One Research Article The intake of carcinogenic and chemopreventive compounds are important nutritional factors related to the development of malignant tumorous diseases. Repetitive long interspersed element-1 (LINE-1) DNA methylation pattern plays a key role in both carcinogenesis and chemoprevention. In our present in vivo animal model, we examined LINE-1 DNA methylation pattern as potential biomarker in the liver, spleen and kidney of mice consuming green tea (Camellia sinensis) extract (catechins 80%), a chinese bayberry (Morella rubra) extract (myricetin 80%), a flavonoid extract (with added resveratrol) and coffee (Coffee arabica) extract. In the organs examined, carcinogen 7,12-dimethylbenz(a)anthracene (DMBA)-induced hypomethylation was prevented by all test materials except chinese bayberry extract in the kidneys. Moreover, the flavonoid extract caused significant hypermethylation in the liver compared to untreated controls and to other test materials. The tested chemopreventive substances have antioxidant, anti-inflammatory properties and regulate molecular biological signaling pathways. They increase glutathione levels, induce antioxidant enzymes, which decrease free radical damage caused by DMBA, and ultimately, they are able to increase the activity of DNA methyltransferase enzymes. Furthermore, flavonoids in the liver may inhibit the procarcinogen to carcinogen activation of DMBA through the inhibition of CYP1A1 enzyme. At the same time, paradoxically, myricetin can act as a prooxidant as a result of free radical damage, which can explain that it did not prevent hypomethylation in the kidneys. Our results demonstrated that LINE-1 DNA methylation pattern is a useful potential biomarker for detecting and monitoring carcinogenic and chemopreventive effects of dietary compounds. Public Library of Science 2021-04-20 /pmc/articles/PMC8057585/ /pubmed/33878138 http://dx.doi.org/10.1371/journal.pone.0250157 Text en © 2021 Szabo et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Szabo, Laszlo
Molnar, Richard
Tomesz, Andras
Deutsch, Arpad
Darago, Richard
Nowrasteh, Ghodratollah
Varjas, Timea
Nemeth, Balazs
Budan, Ferenc
Kiss, Istvan
The effects of flavonoids, green tea polyphenols and coffee on DMBA induced LINE-1 DNA hypomethylation
title The effects of flavonoids, green tea polyphenols and coffee on DMBA induced LINE-1 DNA hypomethylation
title_full The effects of flavonoids, green tea polyphenols and coffee on DMBA induced LINE-1 DNA hypomethylation
title_fullStr The effects of flavonoids, green tea polyphenols and coffee on DMBA induced LINE-1 DNA hypomethylation
title_full_unstemmed The effects of flavonoids, green tea polyphenols and coffee on DMBA induced LINE-1 DNA hypomethylation
title_short The effects of flavonoids, green tea polyphenols and coffee on DMBA induced LINE-1 DNA hypomethylation
title_sort effects of flavonoids, green tea polyphenols and coffee on dmba induced line-1 dna hypomethylation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8057585/
https://www.ncbi.nlm.nih.gov/pubmed/33878138
http://dx.doi.org/10.1371/journal.pone.0250157
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