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The effects of flavonoids, green tea polyphenols and coffee on DMBA induced LINE-1 DNA hypomethylation
The intake of carcinogenic and chemopreventive compounds are important nutritional factors related to the development of malignant tumorous diseases. Repetitive long interspersed element-1 (LINE-1) DNA methylation pattern plays a key role in both carcinogenesis and chemoprevention. In our present in...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8057585/ https://www.ncbi.nlm.nih.gov/pubmed/33878138 http://dx.doi.org/10.1371/journal.pone.0250157 |
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author | Szabo, Laszlo Molnar, Richard Tomesz, Andras Deutsch, Arpad Darago, Richard Nowrasteh, Ghodratollah Varjas, Timea Nemeth, Balazs Budan, Ferenc Kiss, Istvan |
author_facet | Szabo, Laszlo Molnar, Richard Tomesz, Andras Deutsch, Arpad Darago, Richard Nowrasteh, Ghodratollah Varjas, Timea Nemeth, Balazs Budan, Ferenc Kiss, Istvan |
author_sort | Szabo, Laszlo |
collection | PubMed |
description | The intake of carcinogenic and chemopreventive compounds are important nutritional factors related to the development of malignant tumorous diseases. Repetitive long interspersed element-1 (LINE-1) DNA methylation pattern plays a key role in both carcinogenesis and chemoprevention. In our present in vivo animal model, we examined LINE-1 DNA methylation pattern as potential biomarker in the liver, spleen and kidney of mice consuming green tea (Camellia sinensis) extract (catechins 80%), a chinese bayberry (Morella rubra) extract (myricetin 80%), a flavonoid extract (with added resveratrol) and coffee (Coffee arabica) extract. In the organs examined, carcinogen 7,12-dimethylbenz(a)anthracene (DMBA)-induced hypomethylation was prevented by all test materials except chinese bayberry extract in the kidneys. Moreover, the flavonoid extract caused significant hypermethylation in the liver compared to untreated controls and to other test materials. The tested chemopreventive substances have antioxidant, anti-inflammatory properties and regulate molecular biological signaling pathways. They increase glutathione levels, induce antioxidant enzymes, which decrease free radical damage caused by DMBA, and ultimately, they are able to increase the activity of DNA methyltransferase enzymes. Furthermore, flavonoids in the liver may inhibit the procarcinogen to carcinogen activation of DMBA through the inhibition of CYP1A1 enzyme. At the same time, paradoxically, myricetin can act as a prooxidant as a result of free radical damage, which can explain that it did not prevent hypomethylation in the kidneys. Our results demonstrated that LINE-1 DNA methylation pattern is a useful potential biomarker for detecting and monitoring carcinogenic and chemopreventive effects of dietary compounds. |
format | Online Article Text |
id | pubmed-8057585 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-80575852021-05-04 The effects of flavonoids, green tea polyphenols and coffee on DMBA induced LINE-1 DNA hypomethylation Szabo, Laszlo Molnar, Richard Tomesz, Andras Deutsch, Arpad Darago, Richard Nowrasteh, Ghodratollah Varjas, Timea Nemeth, Balazs Budan, Ferenc Kiss, Istvan PLoS One Research Article The intake of carcinogenic and chemopreventive compounds are important nutritional factors related to the development of malignant tumorous diseases. Repetitive long interspersed element-1 (LINE-1) DNA methylation pattern plays a key role in both carcinogenesis and chemoprevention. In our present in vivo animal model, we examined LINE-1 DNA methylation pattern as potential biomarker in the liver, spleen and kidney of mice consuming green tea (Camellia sinensis) extract (catechins 80%), a chinese bayberry (Morella rubra) extract (myricetin 80%), a flavonoid extract (with added resveratrol) and coffee (Coffee arabica) extract. In the organs examined, carcinogen 7,12-dimethylbenz(a)anthracene (DMBA)-induced hypomethylation was prevented by all test materials except chinese bayberry extract in the kidneys. Moreover, the flavonoid extract caused significant hypermethylation in the liver compared to untreated controls and to other test materials. The tested chemopreventive substances have antioxidant, anti-inflammatory properties and regulate molecular biological signaling pathways. They increase glutathione levels, induce antioxidant enzymes, which decrease free radical damage caused by DMBA, and ultimately, they are able to increase the activity of DNA methyltransferase enzymes. Furthermore, flavonoids in the liver may inhibit the procarcinogen to carcinogen activation of DMBA through the inhibition of CYP1A1 enzyme. At the same time, paradoxically, myricetin can act as a prooxidant as a result of free radical damage, which can explain that it did not prevent hypomethylation in the kidneys. Our results demonstrated that LINE-1 DNA methylation pattern is a useful potential biomarker for detecting and monitoring carcinogenic and chemopreventive effects of dietary compounds. Public Library of Science 2021-04-20 /pmc/articles/PMC8057585/ /pubmed/33878138 http://dx.doi.org/10.1371/journal.pone.0250157 Text en © 2021 Szabo et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Szabo, Laszlo Molnar, Richard Tomesz, Andras Deutsch, Arpad Darago, Richard Nowrasteh, Ghodratollah Varjas, Timea Nemeth, Balazs Budan, Ferenc Kiss, Istvan The effects of flavonoids, green tea polyphenols and coffee on DMBA induced LINE-1 DNA hypomethylation |
title | The effects of flavonoids, green tea polyphenols and coffee on DMBA induced LINE-1 DNA hypomethylation |
title_full | The effects of flavonoids, green tea polyphenols and coffee on DMBA induced LINE-1 DNA hypomethylation |
title_fullStr | The effects of flavonoids, green tea polyphenols and coffee on DMBA induced LINE-1 DNA hypomethylation |
title_full_unstemmed | The effects of flavonoids, green tea polyphenols and coffee on DMBA induced LINE-1 DNA hypomethylation |
title_short | The effects of flavonoids, green tea polyphenols and coffee on DMBA induced LINE-1 DNA hypomethylation |
title_sort | effects of flavonoids, green tea polyphenols and coffee on dmba induced line-1 dna hypomethylation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8057585/ https://www.ncbi.nlm.nih.gov/pubmed/33878138 http://dx.doi.org/10.1371/journal.pone.0250157 |
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