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Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals

There is an ongoing need of developing sensitive and specific methods for the determination of SARS-CoV-2 seroconversion. For this purpose, we have developed a multiplexed flow cytometric bead array (C19BA) that allows the identification of IgG and IgM antibodies against three immunogenic proteins s...

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Autores principales: Egia-Mendikute, Leire, Bosch, Alexandre, Prieto-Fernández, Endika, Lee, So Young, Jiménez-Lasheras, Borja, García del Río, Ana, Antoñana-Vildosola, Asier, Bruzzone, Chiara, Bizkarguenaga, Maider, Embade, Nieves, Gil-Redondo, Rubén, Martínez-Chantar, María Luz, López-Hoyos, Marcos, Abrescia, Nicola G. A., Mato, José M., Millet, Óscar, Palazón, Asís
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058339/
https://www.ncbi.nlm.nih.gov/pubmed/33879833
http://dx.doi.org/10.1038/s42003-021-02011-6
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author Egia-Mendikute, Leire
Bosch, Alexandre
Prieto-Fernández, Endika
Lee, So Young
Jiménez-Lasheras, Borja
García del Río, Ana
Antoñana-Vildosola, Asier
Bruzzone, Chiara
Bizkarguenaga, Maider
Embade, Nieves
Gil-Redondo, Rubén
Martínez-Chantar, María Luz
López-Hoyos, Marcos
Abrescia, Nicola G. A.
Mato, José M.
Millet, Óscar
Palazón, Asís
author_facet Egia-Mendikute, Leire
Bosch, Alexandre
Prieto-Fernández, Endika
Lee, So Young
Jiménez-Lasheras, Borja
García del Río, Ana
Antoñana-Vildosola, Asier
Bruzzone, Chiara
Bizkarguenaga, Maider
Embade, Nieves
Gil-Redondo, Rubén
Martínez-Chantar, María Luz
López-Hoyos, Marcos
Abrescia, Nicola G. A.
Mato, José M.
Millet, Óscar
Palazón, Asís
author_sort Egia-Mendikute, Leire
collection PubMed
description There is an ongoing need of developing sensitive and specific methods for the determination of SARS-CoV-2 seroconversion. For this purpose, we have developed a multiplexed flow cytometric bead array (C19BA) that allows the identification of IgG and IgM antibodies against three immunogenic proteins simultaneously: the spike receptor-binding domain (RBD), the spike protein subunit 1 (S1) and the nucleoprotein (N). Using different cohorts of samples collected before and after the pandemic, we show that this assay is more sensitive than ELISAs performed in our laboratory. The combination of three viral antigens allows for the interrogation of full seroconversion. Importantly, we have detected N-reactive antibodies in COVID-19-negative individuals. Here we present an immunoassay that can be easily implemented and has superior potential to detect low antibody titers compared to current gold standard serology methods.
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spelling pubmed-80583392021-05-05 Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals Egia-Mendikute, Leire Bosch, Alexandre Prieto-Fernández, Endika Lee, So Young Jiménez-Lasheras, Borja García del Río, Ana Antoñana-Vildosola, Asier Bruzzone, Chiara Bizkarguenaga, Maider Embade, Nieves Gil-Redondo, Rubén Martínez-Chantar, María Luz López-Hoyos, Marcos Abrescia, Nicola G. A. Mato, José M. Millet, Óscar Palazón, Asís Commun Biol Article There is an ongoing need of developing sensitive and specific methods for the determination of SARS-CoV-2 seroconversion. For this purpose, we have developed a multiplexed flow cytometric bead array (C19BA) that allows the identification of IgG and IgM antibodies against three immunogenic proteins simultaneously: the spike receptor-binding domain (RBD), the spike protein subunit 1 (S1) and the nucleoprotein (N). Using different cohorts of samples collected before and after the pandemic, we show that this assay is more sensitive than ELISAs performed in our laboratory. The combination of three viral antigens allows for the interrogation of full seroconversion. Importantly, we have detected N-reactive antibodies in COVID-19-negative individuals. Here we present an immunoassay that can be easily implemented and has superior potential to detect low antibody titers compared to current gold standard serology methods. Nature Publishing Group UK 2021-04-20 /pmc/articles/PMC8058339/ /pubmed/33879833 http://dx.doi.org/10.1038/s42003-021-02011-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Egia-Mendikute, Leire
Bosch, Alexandre
Prieto-Fernández, Endika
Lee, So Young
Jiménez-Lasheras, Borja
García del Río, Ana
Antoñana-Vildosola, Asier
Bruzzone, Chiara
Bizkarguenaga, Maider
Embade, Nieves
Gil-Redondo, Rubén
Martínez-Chantar, María Luz
López-Hoyos, Marcos
Abrescia, Nicola G. A.
Mato, José M.
Millet, Óscar
Palazón, Asís
Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
title Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
title_full Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
title_fullStr Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
title_full_unstemmed Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
title_short Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
title_sort sensitive detection of sars-cov-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058339/
https://www.ncbi.nlm.nih.gov/pubmed/33879833
http://dx.doi.org/10.1038/s42003-021-02011-6
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