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Interannual dynamics, diversity and evolution of the virome in Sclerotinia sclerotiorum from a single crop field

Mycovirus diversity is generally analyzed from isolates of fungal culture isolates at a single point in time as a snapshot. The stability of mycovirus composition within the same geographical location over time remains unclear. Not knowing how the population fluctuates in the field can be a source o...

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Autores principales: Jia, Jichun, Fu, Yanping, Jiang, Daohong, Mu, Fan, Cheng, Jiasen, Lin, Yang, Li, Bo, Marzano, Shin-Yi Lee, Xie, Jiatao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058396/
https://www.ncbi.nlm.nih.gov/pubmed/33927888
http://dx.doi.org/10.1093/ve/veab032
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author Jia, Jichun
Fu, Yanping
Jiang, Daohong
Mu, Fan
Cheng, Jiasen
Lin, Yang
Li, Bo
Marzano, Shin-Yi Lee
Xie, Jiatao
author_facet Jia, Jichun
Fu, Yanping
Jiang, Daohong
Mu, Fan
Cheng, Jiasen
Lin, Yang
Li, Bo
Marzano, Shin-Yi Lee
Xie, Jiatao
author_sort Jia, Jichun
collection PubMed
description Mycovirus diversity is generally analyzed from isolates of fungal culture isolates at a single point in time as a snapshot. The stability of mycovirus composition within the same geographical location over time remains unclear. Not knowing how the population fluctuates in the field can be a source of unpredictability in the successful application of virocontrol. To better understand the changes over time, we monitored the interannual dynamics and abundance of mycoviruses infecting Sclerotinia sclerotiorum at a rapeseed-growing field for three years. We found that the virome in S. sclerotiorum harbors unique mycovirus compositions each year. In total, sixty-eight mycoviruses were identified, among which twenty-four were detected in all three successive years. These twenty-four mycoviruses can be classified as the members of the core virome in this S. sclerotiorum population, which show persistence and relatively high transmissibility under field conditions. Nearly two-thirds of the mycoviruses have positive-sense, single-stranded RNA genomes and were found consistently across all three years. Moreover, twenty-eight mycoviruses are newly described, including four novel, multi-segmented narnaviruses, and four unique bunyaviruses. Overall, the newly discovered mycoviruses in this study belong to as many as twenty families, into which eight were first identified in S. sclerotiorum, demonstrating evolutionarily diverse viromes. Our findings not only shed light on the annual variation of mycovirus diversity but also provide important virus evolutionary clues.
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spelling pubmed-80583962021-04-28 Interannual dynamics, diversity and evolution of the virome in Sclerotinia sclerotiorum from a single crop field Jia, Jichun Fu, Yanping Jiang, Daohong Mu, Fan Cheng, Jiasen Lin, Yang Li, Bo Marzano, Shin-Yi Lee Xie, Jiatao Virus Evol Research Article Mycovirus diversity is generally analyzed from isolates of fungal culture isolates at a single point in time as a snapshot. The stability of mycovirus composition within the same geographical location over time remains unclear. Not knowing how the population fluctuates in the field can be a source of unpredictability in the successful application of virocontrol. To better understand the changes over time, we monitored the interannual dynamics and abundance of mycoviruses infecting Sclerotinia sclerotiorum at a rapeseed-growing field for three years. We found that the virome in S. sclerotiorum harbors unique mycovirus compositions each year. In total, sixty-eight mycoviruses were identified, among which twenty-four were detected in all three successive years. These twenty-four mycoviruses can be classified as the members of the core virome in this S. sclerotiorum population, which show persistence and relatively high transmissibility under field conditions. Nearly two-thirds of the mycoviruses have positive-sense, single-stranded RNA genomes and were found consistently across all three years. Moreover, twenty-eight mycoviruses are newly described, including four novel, multi-segmented narnaviruses, and four unique bunyaviruses. Overall, the newly discovered mycoviruses in this study belong to as many as twenty families, into which eight were first identified in S. sclerotiorum, demonstrating evolutionarily diverse viromes. Our findings not only shed light on the annual variation of mycovirus diversity but also provide important virus evolutionary clues. Oxford University Press 2021-03-31 /pmc/articles/PMC8058396/ /pubmed/33927888 http://dx.doi.org/10.1093/ve/veab032 Text en © The Author(s) 2021. Published by Oxford University Press. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Article
Jia, Jichun
Fu, Yanping
Jiang, Daohong
Mu, Fan
Cheng, Jiasen
Lin, Yang
Li, Bo
Marzano, Shin-Yi Lee
Xie, Jiatao
Interannual dynamics, diversity and evolution of the virome in Sclerotinia sclerotiorum from a single crop field
title Interannual dynamics, diversity and evolution of the virome in Sclerotinia sclerotiorum from a single crop field
title_full Interannual dynamics, diversity and evolution of the virome in Sclerotinia sclerotiorum from a single crop field
title_fullStr Interannual dynamics, diversity and evolution of the virome in Sclerotinia sclerotiorum from a single crop field
title_full_unstemmed Interannual dynamics, diversity and evolution of the virome in Sclerotinia sclerotiorum from a single crop field
title_short Interannual dynamics, diversity and evolution of the virome in Sclerotinia sclerotiorum from a single crop field
title_sort interannual dynamics, diversity and evolution of the virome in sclerotinia sclerotiorum from a single crop field
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058396/
https://www.ncbi.nlm.nih.gov/pubmed/33927888
http://dx.doi.org/10.1093/ve/veab032
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