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Interactions Between Commensal Microbiota and Mucosal Immunity in Teleost Fish During Viral Infection With SVCV

The mucosa of vertebrates is a particularly complex but dynamic environment in which the host constantly interacts with trillions of commensal microorganisms and pathogens. Although the internal and external mucosal microbiomes with immune defense of mammals have been well investigated, the relation...

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Autores principales: Meng, Kai-Feng, Ding, Li-Guo, Wu, Sha, Wu, Zheng-Ben, Cheng, Gao-Feng, Zhai, Xue, Sun, Ru-Han, Xu, Zhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058427/
https://www.ncbi.nlm.nih.gov/pubmed/33897703
http://dx.doi.org/10.3389/fimmu.2021.654758
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author Meng, Kai-Feng
Ding, Li-Guo
Wu, Sha
Wu, Zheng-Ben
Cheng, Gao-Feng
Zhai, Xue
Sun, Ru-Han
Xu, Zhen
author_facet Meng, Kai-Feng
Ding, Li-Guo
Wu, Sha
Wu, Zheng-Ben
Cheng, Gao-Feng
Zhai, Xue
Sun, Ru-Han
Xu, Zhen
author_sort Meng, Kai-Feng
collection PubMed
description The mucosa of vertebrates is a particularly complex but dynamic environment in which the host constantly interacts with trillions of commensal microorganisms and pathogens. Although the internal and external mucosal microbiomes with immune defense of mammals have been well investigated, the relationship between mucosal microbes and their host’s immune responses has not been systematically understood in the early vertebrates. In this study, we compared the composition and distribution of mucosal microbiota in common carp (Cyprinus carpio), and found that there were significant differences of microbiota between in the internal (gut) and external mucosal (buccal mucosa, gills and skin) tissues. Next, we successfully constructed an infection model with spring viremia of carp virus (SVCV). Specifically, following viral infection, the immune and antiviral related genes showed different up-regulation in all selected mucosal tissues while significant morphological changes were only found in external tissues including buccal mucosa, gills and skin. Using 16S rRNA gene sequence, we revealed that the abundance of Proteobacteria in mucosal tissues including buccal mucosa, gills and gut showed increased trend after viral infection, whereas the abundance of Fusobacteria significantly decreased in gut. In addition, the loss of dominant commensal microorganisms and increased colonization of opportunistic bacteria were discovered in the mucosal surfaces indicating that a secondary bacterial infection might occur in these mucosal tissues after viral infection. Overall, our results firstly point out the distribution of internal and external mucosal microbiota and analyze the changes of mucosal microbiota in common carp after SVCV infection, which may indicated that the potential role of mucosal microbiota in the antiviral process in early vertebrates.
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spelling pubmed-80584272021-04-22 Interactions Between Commensal Microbiota and Mucosal Immunity in Teleost Fish During Viral Infection With SVCV Meng, Kai-Feng Ding, Li-Guo Wu, Sha Wu, Zheng-Ben Cheng, Gao-Feng Zhai, Xue Sun, Ru-Han Xu, Zhen Front Immunol Immunology The mucosa of vertebrates is a particularly complex but dynamic environment in which the host constantly interacts with trillions of commensal microorganisms and pathogens. Although the internal and external mucosal microbiomes with immune defense of mammals have been well investigated, the relationship between mucosal microbes and their host’s immune responses has not been systematically understood in the early vertebrates. In this study, we compared the composition and distribution of mucosal microbiota in common carp (Cyprinus carpio), and found that there were significant differences of microbiota between in the internal (gut) and external mucosal (buccal mucosa, gills and skin) tissues. Next, we successfully constructed an infection model with spring viremia of carp virus (SVCV). Specifically, following viral infection, the immune and antiviral related genes showed different up-regulation in all selected mucosal tissues while significant morphological changes were only found in external tissues including buccal mucosa, gills and skin. Using 16S rRNA gene sequence, we revealed that the abundance of Proteobacteria in mucosal tissues including buccal mucosa, gills and gut showed increased trend after viral infection, whereas the abundance of Fusobacteria significantly decreased in gut. In addition, the loss of dominant commensal microorganisms and increased colonization of opportunistic bacteria were discovered in the mucosal surfaces indicating that a secondary bacterial infection might occur in these mucosal tissues after viral infection. Overall, our results firstly point out the distribution of internal and external mucosal microbiota and analyze the changes of mucosal microbiota in common carp after SVCV infection, which may indicated that the potential role of mucosal microbiota in the antiviral process in early vertebrates. Frontiers Media S.A. 2021-04-07 /pmc/articles/PMC8058427/ /pubmed/33897703 http://dx.doi.org/10.3389/fimmu.2021.654758 Text en Copyright © 2021 Meng, Ding, Wu, Wu, Cheng, Zhai, Sun and Xu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Meng, Kai-Feng
Ding, Li-Guo
Wu, Sha
Wu, Zheng-Ben
Cheng, Gao-Feng
Zhai, Xue
Sun, Ru-Han
Xu, Zhen
Interactions Between Commensal Microbiota and Mucosal Immunity in Teleost Fish During Viral Infection With SVCV
title Interactions Between Commensal Microbiota and Mucosal Immunity in Teleost Fish During Viral Infection With SVCV
title_full Interactions Between Commensal Microbiota and Mucosal Immunity in Teleost Fish During Viral Infection With SVCV
title_fullStr Interactions Between Commensal Microbiota and Mucosal Immunity in Teleost Fish During Viral Infection With SVCV
title_full_unstemmed Interactions Between Commensal Microbiota and Mucosal Immunity in Teleost Fish During Viral Infection With SVCV
title_short Interactions Between Commensal Microbiota and Mucosal Immunity in Teleost Fish During Viral Infection With SVCV
title_sort interactions between commensal microbiota and mucosal immunity in teleost fish during viral infection with svcv
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058427/
https://www.ncbi.nlm.nih.gov/pubmed/33897703
http://dx.doi.org/10.3389/fimmu.2021.654758
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