Cargando…
The Regulation of Adaptation to Cold and Drought Stresses in Poa crymophila Keng Revealed by Integrative Transcriptomics and Metabolomics Analysis
Poa crymophila Keng is highly adaptable to long-term low temperature and drought conditions, making it a desirable foraging grass of the Qinghai-Tibet Plateau. Here, the widely targeted metabolomics and comparative transcriptome analyses were utilized for the discovery of metabolites and genes in P....
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058472/ https://www.ncbi.nlm.nih.gov/pubmed/33897721 http://dx.doi.org/10.3389/fpls.2021.631117 |
_version_ | 1783681021135290368 |
---|---|
author | Wang, Yan Li, Xin-Yu Li, Cai-Xia He, Yuan Hou, Xin-Yi Ma, Xin-Rong |
author_facet | Wang, Yan Li, Xin-Yu Li, Cai-Xia He, Yuan Hou, Xin-Yi Ma, Xin-Rong |
author_sort | Wang, Yan |
collection | PubMed |
description | Poa crymophila Keng is highly adaptable to long-term low temperature and drought conditions, making it a desirable foraging grass of the Qinghai-Tibet Plateau. Here, the widely targeted metabolomics and comparative transcriptome analyses were utilized for the discovery of metabolites and genes in P. crymophila in response to cold and drought stresses. P. crymophila were exposed to −5°C for 24 h and recovered to 22°C for 48 h, as well as drought for 10 days followed by re-watering for 1 day. In total, 779 metabolic features were assigned to metabolites and 167,845 unigenes were generated. Seventeen compounds showed significant up-regulation (variable importance in project >1) under both stresses in the metabolic profiling, mainly annotated as carbohydrates, flavones, and phenylpropanoids. The genes which were positively correlated with these metabolites were assigned to pathways (sucrose-starch, raffinose, phenylpropanoid, and flavone metabolism) using the Mapman software package. Alpha-amylase, beta-fructofuranosidase, and sugar transport genes degraded the glucose and starch to small molecule sugars for the purpose of osmotic adjustment and to provide more energy for the growth of P. crymophila in an adverse environment. The induction of cinnamoyl-CoA reductase (CCR) and the MYB gene as well as the sharp increase in schizandrin, a kind of lignan, showed that this likely has the closest connection with the tolerance to both stresses. Four significantly induced flavone compounds are probably involved in reducing oxidative damage. Our results indicated that activation of the phenlypropanoid pathway plays the primary role in P. crymophila adapting to harsh environments. This study showed the mechanism of P. crymophila responding to both cold and drought stresses and showed the discovery of a new biological regulator against stresses. |
format | Online Article Text |
id | pubmed-8058472 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80584722021-04-22 The Regulation of Adaptation to Cold and Drought Stresses in Poa crymophila Keng Revealed by Integrative Transcriptomics and Metabolomics Analysis Wang, Yan Li, Xin-Yu Li, Cai-Xia He, Yuan Hou, Xin-Yi Ma, Xin-Rong Front Plant Sci Plant Science Poa crymophila Keng is highly adaptable to long-term low temperature and drought conditions, making it a desirable foraging grass of the Qinghai-Tibet Plateau. Here, the widely targeted metabolomics and comparative transcriptome analyses were utilized for the discovery of metabolites and genes in P. crymophila in response to cold and drought stresses. P. crymophila were exposed to −5°C for 24 h and recovered to 22°C for 48 h, as well as drought for 10 days followed by re-watering for 1 day. In total, 779 metabolic features were assigned to metabolites and 167,845 unigenes were generated. Seventeen compounds showed significant up-regulation (variable importance in project >1) under both stresses in the metabolic profiling, mainly annotated as carbohydrates, flavones, and phenylpropanoids. The genes which were positively correlated with these metabolites were assigned to pathways (sucrose-starch, raffinose, phenylpropanoid, and flavone metabolism) using the Mapman software package. Alpha-amylase, beta-fructofuranosidase, and sugar transport genes degraded the glucose and starch to small molecule sugars for the purpose of osmotic adjustment and to provide more energy for the growth of P. crymophila in an adverse environment. The induction of cinnamoyl-CoA reductase (CCR) and the MYB gene as well as the sharp increase in schizandrin, a kind of lignan, showed that this likely has the closest connection with the tolerance to both stresses. Four significantly induced flavone compounds are probably involved in reducing oxidative damage. Our results indicated that activation of the phenlypropanoid pathway plays the primary role in P. crymophila adapting to harsh environments. This study showed the mechanism of P. crymophila responding to both cold and drought stresses and showed the discovery of a new biological regulator against stresses. Frontiers Media S.A. 2021-04-07 /pmc/articles/PMC8058472/ /pubmed/33897721 http://dx.doi.org/10.3389/fpls.2021.631117 Text en Copyright © 2021 Wang, Li, Li, He, Hou and Ma. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Wang, Yan Li, Xin-Yu Li, Cai-Xia He, Yuan Hou, Xin-Yi Ma, Xin-Rong The Regulation of Adaptation to Cold and Drought Stresses in Poa crymophila Keng Revealed by Integrative Transcriptomics and Metabolomics Analysis |
title | The Regulation of Adaptation to Cold and Drought Stresses in Poa crymophila Keng Revealed by Integrative Transcriptomics and Metabolomics Analysis |
title_full | The Regulation of Adaptation to Cold and Drought Stresses in Poa crymophila Keng Revealed by Integrative Transcriptomics and Metabolomics Analysis |
title_fullStr | The Regulation of Adaptation to Cold and Drought Stresses in Poa crymophila Keng Revealed by Integrative Transcriptomics and Metabolomics Analysis |
title_full_unstemmed | The Regulation of Adaptation to Cold and Drought Stresses in Poa crymophila Keng Revealed by Integrative Transcriptomics and Metabolomics Analysis |
title_short | The Regulation of Adaptation to Cold and Drought Stresses in Poa crymophila Keng Revealed by Integrative Transcriptomics and Metabolomics Analysis |
title_sort | regulation of adaptation to cold and drought stresses in poa crymophila keng revealed by integrative transcriptomics and metabolomics analysis |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058472/ https://www.ncbi.nlm.nih.gov/pubmed/33897721 http://dx.doi.org/10.3389/fpls.2021.631117 |
work_keys_str_mv | AT wangyan theregulationofadaptationtocoldanddroughtstressesinpoacrymophilakengrevealedbyintegrativetranscriptomicsandmetabolomicsanalysis AT lixinyu theregulationofadaptationtocoldanddroughtstressesinpoacrymophilakengrevealedbyintegrativetranscriptomicsandmetabolomicsanalysis AT licaixia theregulationofadaptationtocoldanddroughtstressesinpoacrymophilakengrevealedbyintegrativetranscriptomicsandmetabolomicsanalysis AT heyuan theregulationofadaptationtocoldanddroughtstressesinpoacrymophilakengrevealedbyintegrativetranscriptomicsandmetabolomicsanalysis AT houxinyi theregulationofadaptationtocoldanddroughtstressesinpoacrymophilakengrevealedbyintegrativetranscriptomicsandmetabolomicsanalysis AT maxinrong theregulationofadaptationtocoldanddroughtstressesinpoacrymophilakengrevealedbyintegrativetranscriptomicsandmetabolomicsanalysis AT wangyan regulationofadaptationtocoldanddroughtstressesinpoacrymophilakengrevealedbyintegrativetranscriptomicsandmetabolomicsanalysis AT lixinyu regulationofadaptationtocoldanddroughtstressesinpoacrymophilakengrevealedbyintegrativetranscriptomicsandmetabolomicsanalysis AT licaixia regulationofadaptationtocoldanddroughtstressesinpoacrymophilakengrevealedbyintegrativetranscriptomicsandmetabolomicsanalysis AT heyuan regulationofadaptationtocoldanddroughtstressesinpoacrymophilakengrevealedbyintegrativetranscriptomicsandmetabolomicsanalysis AT houxinyi regulationofadaptationtocoldanddroughtstressesinpoacrymophilakengrevealedbyintegrativetranscriptomicsandmetabolomicsanalysis AT maxinrong regulationofadaptationtocoldanddroughtstressesinpoacrymophilakengrevealedbyintegrativetranscriptomicsandmetabolomicsanalysis |