Generating mutant Aedes aegypti mosquitoes using the CRISPR/Cas9 system

Implementation of CRISPR/Cas9 methodologies for mosquito gene editing has not yet become widespread. This protocol details the procedure for Aedes aegypti mosquito gene editing using homology-directed repair and fluorescent marker insertion, which facilitates the generation and screening of mutant m...

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Detalles Bibliográficos
Autores principales: Li, Hsing-Han, Li, Jian-Chiuan, Su, Matthew P., Liu, Kun-Lin, Chen, Chun-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058568/
https://www.ncbi.nlm.nih.gov/pubmed/33899015
http://dx.doi.org/10.1016/j.xpro.2021.100432

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