Activation of RAW264.7 cells by PCp-I, a polysaccharide from Psoralea corylifolia L, through NF-κB/MAPK signalling pathway

PCp-I is a polysaccharide isolated and identified from the Psoralea corylifolia L. by our research group. In this study, the immunomodulatory effects of PCp-I on RAW264.7 cells was evaluated. PCp-I could enhance the level of NO along with up-regulation of iNOS mRNA in RAW264.7 cells. The PCp-I could...

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Autores principales: Wang, Honglin, Xu, Xiaoqing, Yin, Zhenhua, Wang, Mengke, Wang, Baoguang, Ma, Changyang, Wang, Jinmei, Kang, Wenyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Original Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058790/
https://www.ncbi.nlm.nih.gov/pubmed/33855900
http://dx.doi.org/10.1177/20587384211010058
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author Wang, Honglin
Xu, Xiaoqing
Yin, Zhenhua
Wang, Mengke
Wang, Baoguang
Ma, Changyang
Wang, Jinmei
Kang, Wenyi
author_facet Wang, Honglin
Xu, Xiaoqing
Yin, Zhenhua
Wang, Mengke
Wang, Baoguang
Ma, Changyang
Wang, Jinmei
Kang, Wenyi
author_sort Wang, Honglin
collection PubMed
description PCp-I is a polysaccharide isolated and identified from the Psoralea corylifolia L. by our research group. In this study, the immunomodulatory effects of PCp-I on RAW264.7 cells was evaluated. PCp-I could enhance the level of NO along with up-regulation of iNOS mRNA in RAW264.7 cells. The PCp-I could significantly up-regulate the mRNA expression of TNF-α and IL-6 in RAW264.7 cells, and then the expression of TNF-α, IL-6, ROS and the phagocytic activity were increased. Additionally, PCp-I could significantly up-regulate the phosphorylation level of p65, p38, ERK and JNK proteins, which proved that PCp-I could activate the macrophages by MAPKs and NF-κB signalling pathway and the TLR4 may be one of the receptors of PCp-I regulate the RAW264.7 cells.
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spelling pubmed-80587902021-05-04 Activation of RAW264.7 cells by PCp-I, a polysaccharide from Psoralea corylifolia L, through NF-κB/MAPK signalling pathway Wang, Honglin Xu, Xiaoqing Yin, Zhenhua Wang, Mengke Wang, Baoguang Ma, Changyang Wang, Jinmei Kang, Wenyi Int J Immunopathol Pharmacol Original Research Article PCp-I is a polysaccharide isolated and identified from the Psoralea corylifolia L. by our research group. In this study, the immunomodulatory effects of PCp-I on RAW264.7 cells was evaluated. PCp-I could enhance the level of NO along with up-regulation of iNOS mRNA in RAW264.7 cells. The PCp-I could significantly up-regulate the mRNA expression of TNF-α and IL-6 in RAW264.7 cells, and then the expression of TNF-α, IL-6, ROS and the phagocytic activity were increased. Additionally, PCp-I could significantly up-regulate the phosphorylation level of p65, p38, ERK and JNK proteins, which proved that PCp-I could activate the macrophages by MAPKs and NF-κB signalling pathway and the TLR4 may be one of the receptors of PCp-I regulate the RAW264.7 cells. SAGE Publications 2021-04-15 /pmc/articles/PMC8058790/ /pubmed/33855900 http://dx.doi.org/10.1177/20587384211010058 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Research Article
Wang, Honglin
Xu, Xiaoqing
Yin, Zhenhua
Wang, Mengke
Wang, Baoguang
Ma, Changyang
Wang, Jinmei
Kang, Wenyi
Activation of RAW264.7 cells by PCp-I, a polysaccharide from Psoralea corylifolia L, through NF-κB/MAPK signalling pathway
title Activation of RAW264.7 cells by PCp-I, a polysaccharide from Psoralea corylifolia L, through NF-κB/MAPK signalling pathway
title_full Activation of RAW264.7 cells by PCp-I, a polysaccharide from Psoralea corylifolia L, through NF-κB/MAPK signalling pathway
title_fullStr Activation of RAW264.7 cells by PCp-I, a polysaccharide from Psoralea corylifolia L, through NF-κB/MAPK signalling pathway
title_full_unstemmed Activation of RAW264.7 cells by PCp-I, a polysaccharide from Psoralea corylifolia L, through NF-κB/MAPK signalling pathway
title_short Activation of RAW264.7 cells by PCp-I, a polysaccharide from Psoralea corylifolia L, through NF-κB/MAPK signalling pathway
title_sort activation of raw264.7 cells by pcp-i, a polysaccharide from psoralea corylifolia l, through nf-κb/mapk signalling pathway
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058790/
https://www.ncbi.nlm.nih.gov/pubmed/33855900
http://dx.doi.org/10.1177/20587384211010058
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