Contribution of the classical polymerase chain reaction in the diagnosis of a HIV-1 infected patient in Benin: a case report

BACKGROUND: First ambitious target by 2020 of UNAIDS is that 90% of people living with HIV know their HIV status. In people older than 18 months of age, serological confirmation test is recommended to confirm HIV infection. CASE PRESENTATION: Here we report the case of a patient tested positive with...

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Detalles Bibliográficos
Autores principales: Tchiakpe, Edmond, Keke, René K., Vidal, Nicole, Bachabi, Moussa, Gangbo, Flore Armande, Diop‑Ndiaye, Halimatou, Toure‑Kane, Coumba, Yessoufou, Akadiri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Case Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059016/
https://www.ncbi.nlm.nih.gov/pubmed/33882959
http://dx.doi.org/10.1186/s12981-021-00342-2
Descripción
Sumario:BACKGROUND: First ambitious target by 2020 of UNAIDS is that 90% of people living with HIV know their HIV status. In people older than 18 months of age, serological confirmation test is recommended to confirm HIV infection. CASE PRESENTATION: Here we report the case of a patient tested positive with HIV-1, ELISA, Murex(®) Ag⁄Ab Combination assay (OD450 = 0.802 and cutoff-OD = 0.279) and negative by using FIRST RESPONSE HIV1-2.O CARD TEST (version 2.0) RAPID HIV CARD TEST. Viral load performed with Cobas(®) TaqMan(®) 96/Cobas(®) Ampliprep(®) was 6.49log(10.) The virus could be sequenced in partial gag and pol genes and belonged to CRF02_AG clade. CONCLUSION: Conventional PCR is a complementary method for the diagnosis of inconclusive HIV-1 serologies by antibodies.

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