Contribution of the classical polymerase chain reaction in the diagnosis of a HIV-1 infected patient in Benin: a case report

BACKGROUND: First ambitious target by 2020 of UNAIDS is that 90% of people living with HIV know their HIV status. In people older than 18 months of age, serological confirmation test is recommended to confirm HIV infection. CASE PRESENTATION: Here we report the case of a patient tested positive with...

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Autores principales: Tchiakpe, Edmond, Keke, René K., Vidal, Nicole, Bachabi, Moussa, Gangbo, Flore Armande, Diop‑Ndiaye, Halimatou, Toure‑Kane, Coumba, Yessoufou, Akadiri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Case Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059016/
https://www.ncbi.nlm.nih.gov/pubmed/33882959
http://dx.doi.org/10.1186/s12981-021-00342-2
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author Tchiakpe, Edmond
Keke, René K.
Vidal, Nicole
Bachabi, Moussa
Gangbo, Flore Armande
Diop‑Ndiaye, Halimatou
Toure‑Kane, Coumba
Yessoufou, Akadiri
author_facet Tchiakpe, Edmond
Keke, René K.
Vidal, Nicole
Bachabi, Moussa
Gangbo, Flore Armande
Diop‑Ndiaye, Halimatou
Toure‑Kane, Coumba
Yessoufou, Akadiri
author_sort Tchiakpe, Edmond
collection PubMed
description BACKGROUND: First ambitious target by 2020 of UNAIDS is that 90% of people living with HIV know their HIV status. In people older than 18 months of age, serological confirmation test is recommended to confirm HIV infection. CASE PRESENTATION: Here we report the case of a patient tested positive with HIV-1, ELISA, Murex(®) Ag⁄Ab Combination assay (OD450 = 0.802 and cutoff-OD = 0.279) and negative by using FIRST RESPONSE HIV1-2.O CARD TEST (version 2.0) RAPID HIV CARD TEST. Viral load performed with Cobas(®) TaqMan(®) 96/Cobas(®) Ampliprep(®) was 6.49log(10.) The virus could be sequenced in partial gag and pol genes and belonged to CRF02_AG clade. CONCLUSION: Conventional PCR is a complementary method for the diagnosis of inconclusive HIV-1 serologies by antibodies.
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spelling pubmed-80590162021-04-21 Contribution of the classical polymerase chain reaction in the diagnosis of a HIV-1 infected patient in Benin: a case report Tchiakpe, Edmond Keke, René K. Vidal, Nicole Bachabi, Moussa Gangbo, Flore Armande Diop‑Ndiaye, Halimatou Toure‑Kane, Coumba Yessoufou, Akadiri AIDS Res Ther Case Report BACKGROUND: First ambitious target by 2020 of UNAIDS is that 90% of people living with HIV know their HIV status. In people older than 18 months of age, serological confirmation test is recommended to confirm HIV infection. CASE PRESENTATION: Here we report the case of a patient tested positive with HIV-1, ELISA, Murex(®) Ag⁄Ab Combination assay (OD450 = 0.802 and cutoff-OD = 0.279) and negative by using FIRST RESPONSE HIV1-2.O CARD TEST (version 2.0) RAPID HIV CARD TEST. Viral load performed with Cobas(®) TaqMan(®) 96/Cobas(®) Ampliprep(®) was 6.49log(10.) The virus could be sequenced in partial gag and pol genes and belonged to CRF02_AG clade. CONCLUSION: Conventional PCR is a complementary method for the diagnosis of inconclusive HIV-1 serologies by antibodies. BioMed Central 2021-04-21 /pmc/articles/PMC8059016/ /pubmed/33882959 http://dx.doi.org/10.1186/s12981-021-00342-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Case Report
Tchiakpe, Edmond
Keke, René K.
Vidal, Nicole
Bachabi, Moussa
Gangbo, Flore Armande
Diop‑Ndiaye, Halimatou
Toure‑Kane, Coumba
Yessoufou, Akadiri
Contribution of the classical polymerase chain reaction in the diagnosis of a HIV-1 infected patient in Benin: a case report
title Contribution of the classical polymerase chain reaction in the diagnosis of a HIV-1 infected patient in Benin: a case report
title_full Contribution of the classical polymerase chain reaction in the diagnosis of a HIV-1 infected patient in Benin: a case report
title_fullStr Contribution of the classical polymerase chain reaction in the diagnosis of a HIV-1 infected patient in Benin: a case report
title_full_unstemmed Contribution of the classical polymerase chain reaction in the diagnosis of a HIV-1 infected patient in Benin: a case report
title_short Contribution of the classical polymerase chain reaction in the diagnosis of a HIV-1 infected patient in Benin: a case report
title_sort contribution of the classical polymerase chain reaction in the diagnosis of a hiv-1 infected patient in benin: a case report
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059016/
https://www.ncbi.nlm.nih.gov/pubmed/33882959
http://dx.doi.org/10.1186/s12981-021-00342-2
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