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Small RNA sequencing reveals distinct nuclear microRNAs in pig granulosa cells during ovarian follicle growth

Nuclear small RNAs have emerged as an important subset of non-coding RNA species that are capable of regulating gene expression. A type of small RNA, microRNA (miRNA) have been shown to regulate development of the ovarian follicle via canonical targeting and translational repression. Little has been...

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Autores principales: Toms, Derek, Pan, Bo, Bai, Yinshan, Li, Julang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059229/
https://www.ncbi.nlm.nih.gov/pubmed/33879202
http://dx.doi.org/10.1186/s13048-021-00802-3
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author Toms, Derek
Pan, Bo
Bai, Yinshan
Li, Julang
author_facet Toms, Derek
Pan, Bo
Bai, Yinshan
Li, Julang
author_sort Toms, Derek
collection PubMed
description Nuclear small RNAs have emerged as an important subset of non-coding RNA species that are capable of regulating gene expression. A type of small RNA, microRNA (miRNA) have been shown to regulate development of the ovarian follicle via canonical targeting and translational repression. Little has been done to study these molecules at a subcellular level. Using cell fractionation and high throughput sequencing, we surveyed cytoplasmic and nuclear small RNA found in the granulosa cells of the pig ovarian antral preovulatory follicle. Bioinformatics analysis revealed a diverse network of small RNA that differ in their subcellular distribution and implied function. We identified predicted genomic DNA binding sites for nucleus-enriched miRNAs that may potentially be involved in transcriptional regulation. The small nucleolar RNA (snoRNA) SNORA73, known to be involved in steroid synthesis, was also found to be highly enriched in the cytoplasm, suggesting a role for snoRNA species in ovarian function. Taken together, these data provide an important resource to study the small RNAome in ovarian follicles and how they may impact fertility. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13048-021-00802-3.
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spelling pubmed-80592292021-04-21 Small RNA sequencing reveals distinct nuclear microRNAs in pig granulosa cells during ovarian follicle growth Toms, Derek Pan, Bo Bai, Yinshan Li, Julang J Ovarian Res Research Nuclear small RNAs have emerged as an important subset of non-coding RNA species that are capable of regulating gene expression. A type of small RNA, microRNA (miRNA) have been shown to regulate development of the ovarian follicle via canonical targeting and translational repression. Little has been done to study these molecules at a subcellular level. Using cell fractionation and high throughput sequencing, we surveyed cytoplasmic and nuclear small RNA found in the granulosa cells of the pig ovarian antral preovulatory follicle. Bioinformatics analysis revealed a diverse network of small RNA that differ in their subcellular distribution and implied function. We identified predicted genomic DNA binding sites for nucleus-enriched miRNAs that may potentially be involved in transcriptional regulation. The small nucleolar RNA (snoRNA) SNORA73, known to be involved in steroid synthesis, was also found to be highly enriched in the cytoplasm, suggesting a role for snoRNA species in ovarian function. Taken together, these data provide an important resource to study the small RNAome in ovarian follicles and how they may impact fertility. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13048-021-00802-3. BioMed Central 2021-04-20 /pmc/articles/PMC8059229/ /pubmed/33879202 http://dx.doi.org/10.1186/s13048-021-00802-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Toms, Derek
Pan, Bo
Bai, Yinshan
Li, Julang
Small RNA sequencing reveals distinct nuclear microRNAs in pig granulosa cells during ovarian follicle growth
title Small RNA sequencing reveals distinct nuclear microRNAs in pig granulosa cells during ovarian follicle growth
title_full Small RNA sequencing reveals distinct nuclear microRNAs in pig granulosa cells during ovarian follicle growth
title_fullStr Small RNA sequencing reveals distinct nuclear microRNAs in pig granulosa cells during ovarian follicle growth
title_full_unstemmed Small RNA sequencing reveals distinct nuclear microRNAs in pig granulosa cells during ovarian follicle growth
title_short Small RNA sequencing reveals distinct nuclear microRNAs in pig granulosa cells during ovarian follicle growth
title_sort small rna sequencing reveals distinct nuclear micrornas in pig granulosa cells during ovarian follicle growth
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059229/
https://www.ncbi.nlm.nih.gov/pubmed/33879202
http://dx.doi.org/10.1186/s13048-021-00802-3
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