Cargando…
Implantable photonic neural probes for light-sheet fluorescence brain imaging
Significance: Light-sheet fluorescence microscopy (LSFM) is a powerful technique for high-speed volumetric functional imaging. However, in typical light-sheet microscopes, the illumination and collection optics impose significant constraints upon the imaging of non-transparent brain tissues. We demo...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society of Photo-Optical Instrumentation Engineers
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059764/ https://www.ncbi.nlm.nih.gov/pubmed/33898636 http://dx.doi.org/10.1117/1.NPh.8.2.025003 |
_version_ | 1783681239796940800 |
---|---|
author | Sacher, Wesley D. Chen, Fu-Der Moradi-Chameh, Homeira Luo, Xianshu Fomenko, Anton Shah, Prajay T. Lordello, Thomas Liu, Xinyu Almog, Ilan Felts Straguzzi, John N. Fowler, Trevor M. Jung, Youngho Hu, Ting Jeong, Junho Lozano, Andres M. Lo, Patrick Guo-Qiang Valiante, Taufik A. Moreaux, Laurent C. Poon, Joyce K. S. Roukes, Michael L. |
author_facet | Sacher, Wesley D. Chen, Fu-Der Moradi-Chameh, Homeira Luo, Xianshu Fomenko, Anton Shah, Prajay T. Lordello, Thomas Liu, Xinyu Almog, Ilan Felts Straguzzi, John N. Fowler, Trevor M. Jung, Youngho Hu, Ting Jeong, Junho Lozano, Andres M. Lo, Patrick Guo-Qiang Valiante, Taufik A. Moreaux, Laurent C. Poon, Joyce K. S. Roukes, Michael L. |
author_sort | Sacher, Wesley D. |
collection | PubMed |
description | Significance: Light-sheet fluorescence microscopy (LSFM) is a powerful technique for high-speed volumetric functional imaging. However, in typical light-sheet microscopes, the illumination and collection optics impose significant constraints upon the imaging of non-transparent brain tissues. We demonstrate that these constraints can be surmounted using a new class of implantable photonic neural probes. Aim: Mass manufacturable, silicon-based light-sheet photonic neural probes can generate planar patterned illumination at arbitrary depths in brain tissues without any additional micro-optic components. Approach: We develop implantable photonic neural probes that generate light sheets in tissue. The probes were fabricated in a photonics foundry on 200-mm-diameter silicon wafers. The light sheets were characterized in fluorescein and in free space. The probe-enabled imaging approach was tested in fixed, in vitro, and in vivo mouse brain tissues. Imaging tests were also performed using fluorescent beads suspended in agarose. Results: The probes had 5 to 10 addressable sheets and average sheet thicknesses [Formula: see text] for propagation distances up to [Formula: see text] in free space. Imaging areas were as large as [Formula: see text] in brain tissue. Image contrast was enhanced relative to epifluorescence microscopy. Conclusions: The neural probes can lead to new variants of LSFM for deep brain imaging and experiments in freely moving animals. |
format | Online Article Text |
id | pubmed-8059764 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Society of Photo-Optical Instrumentation Engineers |
record_format | MEDLINE/PubMed |
spelling | pubmed-80597642021-04-23 Implantable photonic neural probes for light-sheet fluorescence brain imaging Sacher, Wesley D. Chen, Fu-Der Moradi-Chameh, Homeira Luo, Xianshu Fomenko, Anton Shah, Prajay T. Lordello, Thomas Liu, Xinyu Almog, Ilan Felts Straguzzi, John N. Fowler, Trevor M. Jung, Youngho Hu, Ting Jeong, Junho Lozano, Andres M. Lo, Patrick Guo-Qiang Valiante, Taufik A. Moreaux, Laurent C. Poon, Joyce K. S. Roukes, Michael L. Neurophotonics Research Papers Significance: Light-sheet fluorescence microscopy (LSFM) is a powerful technique for high-speed volumetric functional imaging. However, in typical light-sheet microscopes, the illumination and collection optics impose significant constraints upon the imaging of non-transparent brain tissues. We demonstrate that these constraints can be surmounted using a new class of implantable photonic neural probes. Aim: Mass manufacturable, silicon-based light-sheet photonic neural probes can generate planar patterned illumination at arbitrary depths in brain tissues without any additional micro-optic components. Approach: We develop implantable photonic neural probes that generate light sheets in tissue. The probes were fabricated in a photonics foundry on 200-mm-diameter silicon wafers. The light sheets were characterized in fluorescein and in free space. The probe-enabled imaging approach was tested in fixed, in vitro, and in vivo mouse brain tissues. Imaging tests were also performed using fluorescent beads suspended in agarose. Results: The probes had 5 to 10 addressable sheets and average sheet thicknesses [Formula: see text] for propagation distances up to [Formula: see text] in free space. Imaging areas were as large as [Formula: see text] in brain tissue. Image contrast was enhanced relative to epifluorescence microscopy. Conclusions: The neural probes can lead to new variants of LSFM for deep brain imaging and experiments in freely moving animals. Society of Photo-Optical Instrumentation Engineers 2021-04-19 2021-04 /pmc/articles/PMC8059764/ /pubmed/33898636 http://dx.doi.org/10.1117/1.NPh.8.2.025003 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. |
spellingShingle | Research Papers Sacher, Wesley D. Chen, Fu-Der Moradi-Chameh, Homeira Luo, Xianshu Fomenko, Anton Shah, Prajay T. Lordello, Thomas Liu, Xinyu Almog, Ilan Felts Straguzzi, John N. Fowler, Trevor M. Jung, Youngho Hu, Ting Jeong, Junho Lozano, Andres M. Lo, Patrick Guo-Qiang Valiante, Taufik A. Moreaux, Laurent C. Poon, Joyce K. S. Roukes, Michael L. Implantable photonic neural probes for light-sheet fluorescence brain imaging |
title | Implantable photonic neural probes for light-sheet fluorescence brain imaging |
title_full | Implantable photonic neural probes for light-sheet fluorescence brain imaging |
title_fullStr | Implantable photonic neural probes for light-sheet fluorescence brain imaging |
title_full_unstemmed | Implantable photonic neural probes for light-sheet fluorescence brain imaging |
title_short | Implantable photonic neural probes for light-sheet fluorescence brain imaging |
title_sort | implantable photonic neural probes for light-sheet fluorescence brain imaging |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059764/ https://www.ncbi.nlm.nih.gov/pubmed/33898636 http://dx.doi.org/10.1117/1.NPh.8.2.025003 |
work_keys_str_mv | AT sacherwesleyd implantablephotonicneuralprobesforlightsheetfluorescencebrainimaging AT chenfuder implantablephotonicneuralprobesforlightsheetfluorescencebrainimaging AT moradichamehhomeira implantablephotonicneuralprobesforlightsheetfluorescencebrainimaging AT luoxianshu implantablephotonicneuralprobesforlightsheetfluorescencebrainimaging AT fomenkoanton implantablephotonicneuralprobesforlightsheetfluorescencebrainimaging AT shahprajayt implantablephotonicneuralprobesforlightsheetfluorescencebrainimaging AT lordellothomas implantablephotonicneuralprobesforlightsheetfluorescencebrainimaging AT liuxinyu implantablephotonicneuralprobesforlightsheetfluorescencebrainimaging AT almogilanfelts implantablephotonicneuralprobesforlightsheetfluorescencebrainimaging AT straguzzijohnn implantablephotonicneuralprobesforlightsheetfluorescencebrainimaging AT fowlertrevorm implantablephotonicneuralprobesforlightsheetfluorescencebrainimaging AT jungyoungho implantablephotonicneuralprobesforlightsheetfluorescencebrainimaging AT huting implantablephotonicneuralprobesforlightsheetfluorescencebrainimaging AT jeongjunho implantablephotonicneuralprobesforlightsheetfluorescencebrainimaging AT lozanoandresm implantablephotonicneuralprobesforlightsheetfluorescencebrainimaging AT lopatrickguoqiang implantablephotonicneuralprobesforlightsheetfluorescencebrainimaging AT valiantetaufika implantablephotonicneuralprobesforlightsheetfluorescencebrainimaging AT moreauxlaurentc implantablephotonicneuralprobesforlightsheetfluorescencebrainimaging AT poonjoyceks implantablephotonicneuralprobesforlightsheetfluorescencebrainimaging AT roukesmichaell implantablephotonicneuralprobesforlightsheetfluorescencebrainimaging |