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Understanding the Shift in the Microbiome of Composts That Are Optimized for a Better Fit-for-Purpose in Growing Media

Three characteristics are considered key for optimal use of composts in growing media: maturity, pH and organic matter content. Maturation is a critical step in the processing of composts contributing to compost quality. Blending of composts with chopped heath biomass, sieving out the larger fractio...

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Autores principales: Pot, Steffi, De Tender, Caroline, Ommeslag, Sarah, Delcour, Ilse, Ceusters, Johan, Gorrens, Ellen, Debode, Jane, Vandecasteele, Bart, Vancampenhout, Karen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059793/
https://www.ncbi.nlm.nih.gov/pubmed/33897654
http://dx.doi.org/10.3389/fmicb.2021.643679
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author Pot, Steffi
De Tender, Caroline
Ommeslag, Sarah
Delcour, Ilse
Ceusters, Johan
Gorrens, Ellen
Debode, Jane
Vandecasteele, Bart
Vancampenhout, Karen
author_facet Pot, Steffi
De Tender, Caroline
Ommeslag, Sarah
Delcour, Ilse
Ceusters, Johan
Gorrens, Ellen
Debode, Jane
Vandecasteele, Bart
Vancampenhout, Karen
author_sort Pot, Steffi
collection PubMed
description Three characteristics are considered key for optimal use of composts in growing media: maturity, pH and organic matter content. Maturation is a critical step in the processing of composts contributing to compost quality. Blending of composts with chopped heath biomass, sieving out the larger fraction of composts and acidification of composts by adding elemental sulfur may be used either to increase organic matter content or to reduce pH for a better fit in growing media. While several studies have shown the effectiveness of these treatments to improve the use of composts in growing media, the effect of these treatments on the compost microbiome has merely been assessed before. In the present study, five immature composts were allowed to mature, and were subsequently acidified, blended or sieved. Bacterial and fungal communities of the composts were characterized and quantified using 16S rRNA and ITS2 gene metabarcoding and phospholipid fatty acid analysis. Metabolic biodiversity and activity were analyzed using Biolog EcoPlates. Compost batch was shown to be more important than maturation or optimization treatments to determine the compost microbiome. Compost maturation increased microbial diversity and favored beneficial microorganisms, which may be positive for the use of composts in growing media. Blending of composts increased microbial diversity, metabolic diversity, and metabolic activity, which may have a positive effect in growing media. Blending may be used to modify the microbiome to a certain degree in order to optimize microbiological characteristics. Acidification caused a decrease in bacterial diversity and microbial activity, which may be negative for the use in growing media, although the changes are limited. Sieving had limited effect on the microbiome of composts. Because of the limited effect on the microbiome, sieving of composts may be used flexible to improve (bio)chemical characteristics. This is the first study to assess the effects of maturation and optimization treatments to either increase organic matter content or lower pH in composts on the compost microbiome.
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spelling pubmed-80597932021-04-22 Understanding the Shift in the Microbiome of Composts That Are Optimized for a Better Fit-for-Purpose in Growing Media Pot, Steffi De Tender, Caroline Ommeslag, Sarah Delcour, Ilse Ceusters, Johan Gorrens, Ellen Debode, Jane Vandecasteele, Bart Vancampenhout, Karen Front Microbiol Microbiology Three characteristics are considered key for optimal use of composts in growing media: maturity, pH and organic matter content. Maturation is a critical step in the processing of composts contributing to compost quality. Blending of composts with chopped heath biomass, sieving out the larger fraction of composts and acidification of composts by adding elemental sulfur may be used either to increase organic matter content or to reduce pH for a better fit in growing media. While several studies have shown the effectiveness of these treatments to improve the use of composts in growing media, the effect of these treatments on the compost microbiome has merely been assessed before. In the present study, five immature composts were allowed to mature, and were subsequently acidified, blended or sieved. Bacterial and fungal communities of the composts were characterized and quantified using 16S rRNA and ITS2 gene metabarcoding and phospholipid fatty acid analysis. Metabolic biodiversity and activity were analyzed using Biolog EcoPlates. Compost batch was shown to be more important than maturation or optimization treatments to determine the compost microbiome. Compost maturation increased microbial diversity and favored beneficial microorganisms, which may be positive for the use of composts in growing media. Blending of composts increased microbial diversity, metabolic diversity, and metabolic activity, which may have a positive effect in growing media. Blending may be used to modify the microbiome to a certain degree in order to optimize microbiological characteristics. Acidification caused a decrease in bacterial diversity and microbial activity, which may be negative for the use in growing media, although the changes are limited. Sieving had limited effect on the microbiome of composts. Because of the limited effect on the microbiome, sieving of composts may be used flexible to improve (bio)chemical characteristics. This is the first study to assess the effects of maturation and optimization treatments to either increase organic matter content or lower pH in composts on the compost microbiome. Frontiers Media S.A. 2021-04-07 /pmc/articles/PMC8059793/ /pubmed/33897654 http://dx.doi.org/10.3389/fmicb.2021.643679 Text en Copyright © 2021 Pot, De Tender, Ommeslag, Delcour, Ceusters, Gorrens, Debode, Vandecasteele and Vancampenhout. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Pot, Steffi
De Tender, Caroline
Ommeslag, Sarah
Delcour, Ilse
Ceusters, Johan
Gorrens, Ellen
Debode, Jane
Vandecasteele, Bart
Vancampenhout, Karen
Understanding the Shift in the Microbiome of Composts That Are Optimized for a Better Fit-for-Purpose in Growing Media
title Understanding the Shift in the Microbiome of Composts That Are Optimized for a Better Fit-for-Purpose in Growing Media
title_full Understanding the Shift in the Microbiome of Composts That Are Optimized for a Better Fit-for-Purpose in Growing Media
title_fullStr Understanding the Shift in the Microbiome of Composts That Are Optimized for a Better Fit-for-Purpose in Growing Media
title_full_unstemmed Understanding the Shift in the Microbiome of Composts That Are Optimized for a Better Fit-for-Purpose in Growing Media
title_short Understanding the Shift in the Microbiome of Composts That Are Optimized for a Better Fit-for-Purpose in Growing Media
title_sort understanding the shift in the microbiome of composts that are optimized for a better fit-for-purpose in growing media
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059793/
https://www.ncbi.nlm.nih.gov/pubmed/33897654
http://dx.doi.org/10.3389/fmicb.2021.643679
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