Colorimetric isothermal nucleic acid detection of SARS-CoV-2 with dye combination

RT-LAMP detection of SARS-CoV-2 has been demonstrated to be a valuable diagnostic method for the diagnosis of COVID-19(1,2), which can rapidly screen carriers of the virus to effectively control the spread of the SARS-CoV-2. Here, we present a combination of dyes for isothermal detection of SARS-CoV...

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Detalles Bibliográficos
Autores principales: Wu, Shanshan, Liu, Xiyang, Ye, Shenglong, Liu, Jianmin, Zheng, Wei, Dong, Xue, Yin, Xiushan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059943/
https://www.ncbi.nlm.nih.gov/pubmed/33903853
http://dx.doi.org/10.1016/j.heliyon.2021.e06886
Descripción
Sumario:RT-LAMP detection of SARS-CoV-2 has been demonstrated to be a valuable diagnostic method for the diagnosis of COVID-19(1,2), which can rapidly screen carriers of the virus to effectively control the spread of the SARS-CoV-2. Here, we present a combination of dyes for isothermal detection of SARS-CoV-2 as a commercial alternative, with expanded colorimetric spectrum. We compared them with commercial reagents and proved their suitability and sensitivity through clinical RNA samples. In addition, together with commercial single dye indicators, we believe the expanded color spectrum developed here as an indicator of rapid detection will promote the diagnosis of COVID-19.

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