Colorimetric isothermal nucleic acid detection of SARS-CoV-2 with dye combination

RT-LAMP detection of SARS-CoV-2 has been demonstrated to be a valuable diagnostic method for the diagnosis of COVID-19(1,2), which can rapidly screen carriers of the virus to effectively control the spread of the SARS-CoV-2. Here, we present a combination of dyes for isothermal detection of SARS-CoV...

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Autores principales: Wu, Shanshan, Liu, Xiyang, Ye, Shenglong, Liu, Jianmin, Zheng, Wei, Dong, Xue, Yin, Xiushan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059943/
https://www.ncbi.nlm.nih.gov/pubmed/33903853
http://dx.doi.org/10.1016/j.heliyon.2021.e06886
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author Wu, Shanshan
Liu, Xiyang
Ye, Shenglong
Liu, Jianmin
Zheng, Wei
Dong, Xue
Yin, Xiushan
author_facet Wu, Shanshan
Liu, Xiyang
Ye, Shenglong
Liu, Jianmin
Zheng, Wei
Dong, Xue
Yin, Xiushan
author_sort Wu, Shanshan
collection PubMed
description RT-LAMP detection of SARS-CoV-2 has been demonstrated to be a valuable diagnostic method for the diagnosis of COVID-19(1,2), which can rapidly screen carriers of the virus to effectively control the spread of the SARS-CoV-2. Here, we present a combination of dyes for isothermal detection of SARS-CoV-2 as a commercial alternative, with expanded colorimetric spectrum. We compared them with commercial reagents and proved their suitability and sensitivity through clinical RNA samples. In addition, together with commercial single dye indicators, we believe the expanded color spectrum developed here as an indicator of rapid detection will promote the diagnosis of COVID-19.
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spelling pubmed-80599432021-04-22 Colorimetric isothermal nucleic acid detection of SARS-CoV-2 with dye combination Wu, Shanshan Liu, Xiyang Ye, Shenglong Liu, Jianmin Zheng, Wei Dong, Xue Yin, Xiushan Heliyon Research Article RT-LAMP detection of SARS-CoV-2 has been demonstrated to be a valuable diagnostic method for the diagnosis of COVID-19(1,2), which can rapidly screen carriers of the virus to effectively control the spread of the SARS-CoV-2. Here, we present a combination of dyes for isothermal detection of SARS-CoV-2 as a commercial alternative, with expanded colorimetric spectrum. We compared them with commercial reagents and proved their suitability and sensitivity through clinical RNA samples. In addition, together with commercial single dye indicators, we believe the expanded color spectrum developed here as an indicator of rapid detection will promote the diagnosis of COVID-19. Elsevier 2021-04-21 /pmc/articles/PMC8059943/ /pubmed/33903853 http://dx.doi.org/10.1016/j.heliyon.2021.e06886 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Wu, Shanshan
Liu, Xiyang
Ye, Shenglong
Liu, Jianmin
Zheng, Wei
Dong, Xue
Yin, Xiushan
Colorimetric isothermal nucleic acid detection of SARS-CoV-2 with dye combination
title Colorimetric isothermal nucleic acid detection of SARS-CoV-2 with dye combination
title_full Colorimetric isothermal nucleic acid detection of SARS-CoV-2 with dye combination
title_fullStr Colorimetric isothermal nucleic acid detection of SARS-CoV-2 with dye combination
title_full_unstemmed Colorimetric isothermal nucleic acid detection of SARS-CoV-2 with dye combination
title_short Colorimetric isothermal nucleic acid detection of SARS-CoV-2 with dye combination
title_sort colorimetric isothermal nucleic acid detection of sars-cov-2 with dye combination
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059943/
https://www.ncbi.nlm.nih.gov/pubmed/33903853
http://dx.doi.org/10.1016/j.heliyon.2021.e06886
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