miR-34a-5p might have an important role for inducing apoptosis by down-regulation of SNAI1 in apigenin-treated lung cancer cells

Apigenin is a flavonoid with antioxidant and anticancer effects. It has been reported that apigenin inhibits proliferation, migration, and invasion and induces apoptosis in cultured lung cancer cells. However, there is little information on the involvement of microRNAs (miRNAs) in its effects. miRNA...

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Autores principales: Aida, Rieko, Hagiwara, Keitaro, Okano, Kazunori, Nakata, Kyoko, Obata, Yuho, Yamashita, Takahiro, Yoshida, Kaoru, Hagiwara, Hiromi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2021
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Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060201/
https://www.ncbi.nlm.nih.gov/pubmed/33675467
http://dx.doi.org/10.1007/s11033-021-06255-7
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author Aida, Rieko
Hagiwara, Keitaro
Okano, Kazunori
Nakata, Kyoko
Obata, Yuho
Yamashita, Takahiro
Yoshida, Kaoru
Hagiwara, Hiromi
author_facet Aida, Rieko
Hagiwara, Keitaro
Okano, Kazunori
Nakata, Kyoko
Obata, Yuho
Yamashita, Takahiro
Yoshida, Kaoru
Hagiwara, Hiromi
author_sort Aida, Rieko
collection PubMed
description Apigenin is a flavonoid with antioxidant and anticancer effects. It has been reported that apigenin inhibits proliferation, migration, and invasion and induces apoptosis in cultured lung cancer cells. However, there is little information on the involvement of microRNAs (miRNAs) in its effects. miRNA microarray analysis and polymerase-chain-reaction analysis of miRNAs revealed that treatment of human lung cancer A549 cells with apigenin up-regulated the level of miR-34a-5p. Furthermore, mRNA microarray analysis and the results of three microRNA target prediction tools showed that Snail Family Transcriptional Repressor 1 (SNAI1), which inhibits the induction of apoptosis, had its mRNA expression down-regulated in A549 cells treated with apigenin. Our findings suggest that apigenin might induce apoptosis by down-regulation of SNAI1 through up-regulation of miR-34a-5p in A549 cells.
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spelling pubmed-80602012021-05-05 miR-34a-5p might have an important role for inducing apoptosis by down-regulation of SNAI1 in apigenin-treated lung cancer cells Aida, Rieko Hagiwara, Keitaro Okano, Kazunori Nakata, Kyoko Obata, Yuho Yamashita, Takahiro Yoshida, Kaoru Hagiwara, Hiromi Mol Biol Rep Original Article Apigenin is a flavonoid with antioxidant and anticancer effects. It has been reported that apigenin inhibits proliferation, migration, and invasion and induces apoptosis in cultured lung cancer cells. However, there is little information on the involvement of microRNAs (miRNAs) in its effects. miRNA microarray analysis and polymerase-chain-reaction analysis of miRNAs revealed that treatment of human lung cancer A549 cells with apigenin up-regulated the level of miR-34a-5p. Furthermore, mRNA microarray analysis and the results of three microRNA target prediction tools showed that Snail Family Transcriptional Repressor 1 (SNAI1), which inhibits the induction of apoptosis, had its mRNA expression down-regulated in A549 cells treated with apigenin. Our findings suggest that apigenin might induce apoptosis by down-regulation of SNAI1 through up-regulation of miR-34a-5p in A549 cells. Springer Netherlands 2021-03-06 2021 /pmc/articles/PMC8060201/ /pubmed/33675467 http://dx.doi.org/10.1007/s11033-021-06255-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Aida, Rieko
Hagiwara, Keitaro
Okano, Kazunori
Nakata, Kyoko
Obata, Yuho
Yamashita, Takahiro
Yoshida, Kaoru
Hagiwara, Hiromi
miR-34a-5p might have an important role for inducing apoptosis by down-regulation of SNAI1 in apigenin-treated lung cancer cells
title miR-34a-5p might have an important role for inducing apoptosis by down-regulation of SNAI1 in apigenin-treated lung cancer cells
title_full miR-34a-5p might have an important role for inducing apoptosis by down-regulation of SNAI1 in apigenin-treated lung cancer cells
title_fullStr miR-34a-5p might have an important role for inducing apoptosis by down-regulation of SNAI1 in apigenin-treated lung cancer cells
title_full_unstemmed miR-34a-5p might have an important role for inducing apoptosis by down-regulation of SNAI1 in apigenin-treated lung cancer cells
title_short miR-34a-5p might have an important role for inducing apoptosis by down-regulation of SNAI1 in apigenin-treated lung cancer cells
title_sort mir-34a-5p might have an important role for inducing apoptosis by down-regulation of snai1 in apigenin-treated lung cancer cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060201/
https://www.ncbi.nlm.nih.gov/pubmed/33675467
http://dx.doi.org/10.1007/s11033-021-06255-7
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