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Optical density based quantification of total haemoglobin concentrations with spectroscopic optical coherence tomography
Spectroscopic optical coherence tomography (sOCT) has emerged as a new possibility for non-invasive quantification of total haemoglobin concentrations [tHb]. Recently, we demonstrated that [tHb] measured in ex-vivo human whole-blood with a conventional sOCT system achieves a precision of 9.10 g/dL w...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060256/ https://www.ncbi.nlm.nih.gov/pubmed/33883617 http://dx.doi.org/10.1038/s41598-021-88063-4 |
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author | Cuartas-Vélez, Carlos Veenstra, Colin Kruitwagen, Saskia Petersen, Wilma Bosschaart, Nienke |
author_facet | Cuartas-Vélez, Carlos Veenstra, Colin Kruitwagen, Saskia Petersen, Wilma Bosschaart, Nienke |
author_sort | Cuartas-Vélez, Carlos |
collection | PubMed |
description | Spectroscopic optical coherence tomography (sOCT) has emerged as a new possibility for non-invasive quantification of total haemoglobin concentrations [tHb]. Recently, we demonstrated that [tHb] measured in ex-vivo human whole-blood with a conventional sOCT system achieves a precision of 9.10 g/dL with a bias of 1.50 g/dL. This precision improved by acquiring data with a combination of focus tracking and zero-delay acquisition (FZA) that compensated for experimental limitations, increasing to 3.80 g/dL with a bias of 1.50 g/dL. Nevertheless, sOCT precision should improve at least to [Formula: see text] g/dL to be clinically relevant. Therefore, sOCT-based [tHb] determinations require the development of new analysis methods that reduce the variability of [tHb] estimations. In this work, we aim to increase sOCT precision by retrieving the [tHb] content from a numerical optimisation of the optical density (OD), while considering the blood absorption flattening effect. The OD-based approach simplifies previous two-step Lambert–Beer fitting approaches to a single step, thereby reducing errors during the fitting procedure. We validated our model with ex-vivo [tHb] measurements on flowing whole-blood samples in the clinical range (7–23 g/dL). Our results show that, with the new model, conventional sOCT can determine [tHb] with a precision of 3.09 g/dL and a bias of 0.86 g/dL compared to a commercial blood analyser. We present further precision improvement by combining the OD methodology with FZA, leading to a precision of 2.08 g/dL with a bias of 0.46 g/dL. |
format | Online Article Text |
id | pubmed-8060256 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-80602562021-04-22 Optical density based quantification of total haemoglobin concentrations with spectroscopic optical coherence tomography Cuartas-Vélez, Carlos Veenstra, Colin Kruitwagen, Saskia Petersen, Wilma Bosschaart, Nienke Sci Rep Article Spectroscopic optical coherence tomography (sOCT) has emerged as a new possibility for non-invasive quantification of total haemoglobin concentrations [tHb]. Recently, we demonstrated that [tHb] measured in ex-vivo human whole-blood with a conventional sOCT system achieves a precision of 9.10 g/dL with a bias of 1.50 g/dL. This precision improved by acquiring data with a combination of focus tracking and zero-delay acquisition (FZA) that compensated for experimental limitations, increasing to 3.80 g/dL with a bias of 1.50 g/dL. Nevertheless, sOCT precision should improve at least to [Formula: see text] g/dL to be clinically relevant. Therefore, sOCT-based [tHb] determinations require the development of new analysis methods that reduce the variability of [tHb] estimations. In this work, we aim to increase sOCT precision by retrieving the [tHb] content from a numerical optimisation of the optical density (OD), while considering the blood absorption flattening effect. The OD-based approach simplifies previous two-step Lambert–Beer fitting approaches to a single step, thereby reducing errors during the fitting procedure. We validated our model with ex-vivo [tHb] measurements on flowing whole-blood samples in the clinical range (7–23 g/dL). Our results show that, with the new model, conventional sOCT can determine [tHb] with a precision of 3.09 g/dL and a bias of 0.86 g/dL compared to a commercial blood analyser. We present further precision improvement by combining the OD methodology with FZA, leading to a precision of 2.08 g/dL with a bias of 0.46 g/dL. Nature Publishing Group UK 2021-04-21 /pmc/articles/PMC8060256/ /pubmed/33883617 http://dx.doi.org/10.1038/s41598-021-88063-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Cuartas-Vélez, Carlos Veenstra, Colin Kruitwagen, Saskia Petersen, Wilma Bosschaart, Nienke Optical density based quantification of total haemoglobin concentrations with spectroscopic optical coherence tomography |
title | Optical density based quantification of total haemoglobin concentrations with spectroscopic optical coherence tomography |
title_full | Optical density based quantification of total haemoglobin concentrations with spectroscopic optical coherence tomography |
title_fullStr | Optical density based quantification of total haemoglobin concentrations with spectroscopic optical coherence tomography |
title_full_unstemmed | Optical density based quantification of total haemoglobin concentrations with spectroscopic optical coherence tomography |
title_short | Optical density based quantification of total haemoglobin concentrations with spectroscopic optical coherence tomography |
title_sort | optical density based quantification of total haemoglobin concentrations with spectroscopic optical coherence tomography |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060256/ https://www.ncbi.nlm.nih.gov/pubmed/33883617 http://dx.doi.org/10.1038/s41598-021-88063-4 |
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