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Environmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis
Macroalgae host various symbionts on their surface, which play a critical role in their growth and development processes. However, there is still incomplete understanding of this epiphytic bacteria-host algae interactions. This study comprehensively analysed variation of the epiphytic bacterial comm...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060329/ https://www.ncbi.nlm.nih.gov/pubmed/33883606 http://dx.doi.org/10.1038/s41598-021-87977-3 |
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author | Pei, Pengbing Aslam, Muhammad Du, Hong Liang, Honghao Wang, Hui Liu, Xiaojuan Chen, Weizhou |
author_facet | Pei, Pengbing Aslam, Muhammad Du, Hong Liang, Honghao Wang, Hui Liu, Xiaojuan Chen, Weizhou |
author_sort | Pei, Pengbing |
collection | PubMed |
description | Macroalgae host various symbionts on their surface, which play a critical role in their growth and development processes. However, there is still incomplete understanding of this epiphytic bacteria-host algae interactions. This study comprehensively analysed variation of the epiphytic bacterial communities (EBC) composition of red macroalga Gracilariopsis lemaneiformis at different geographic locations and environmental factors (i.e., nitrogen and phosphorus), which shape the EBC composition of G. lemaneiformis. The composition and structure of EBC were characterized using high throughput sequencing of the V3-V4 hypervariable region of the 16S rRNA gene. The results revealed that epiphytic bacteria varied significantly among three different geographic locations in China, i.e., Nan’ao Island (NA), Lianjiang County (LJ), and Nanri Island (NR). Redundancy analysis (RDA) showed that the relative abundance of Bacteroidetes, Firmicutes, Verrucomicrobia, and Epsilonbacteraeota at NR were strongly positively correlated with total nitrogen (TN), total phosphorus (TP), nitrate nitrogen (NO(3)-N), and dissolved inorganic nitrogen (DIN), but negatively correlated with nitrite nitrogen (NO(2)-N). The relative abundance of Cyanobacteria at NA and LJ were strongly positively correlated with NO(2)-N, but negatively correlated with TN, TP, NO(3)-N, and DIN. Besides, the Mantel test results indicated that the EBC composition was significantly correlated with these environmental factors, which was also confirmed by Spearman correlation analysis. Thus, environmental factors such as NO(3)-N and DIN play a key role in the community composition of epiphytic bacteria on G. lemaneiformis. This study provides important baseline knowledge on the community composition of epiphytic bacteria on G. lemaneiformis and shows correlation between different epiphytic bacteria and their surrounding environmental factors. |
format | Online Article Text |
id | pubmed-8060329 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-80603292021-04-23 Environmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis Pei, Pengbing Aslam, Muhammad Du, Hong Liang, Honghao Wang, Hui Liu, Xiaojuan Chen, Weizhou Sci Rep Article Macroalgae host various symbionts on their surface, which play a critical role in their growth and development processes. However, there is still incomplete understanding of this epiphytic bacteria-host algae interactions. This study comprehensively analysed variation of the epiphytic bacterial communities (EBC) composition of red macroalga Gracilariopsis lemaneiformis at different geographic locations and environmental factors (i.e., nitrogen and phosphorus), which shape the EBC composition of G. lemaneiformis. The composition and structure of EBC were characterized using high throughput sequencing of the V3-V4 hypervariable region of the 16S rRNA gene. The results revealed that epiphytic bacteria varied significantly among three different geographic locations in China, i.e., Nan’ao Island (NA), Lianjiang County (LJ), and Nanri Island (NR). Redundancy analysis (RDA) showed that the relative abundance of Bacteroidetes, Firmicutes, Verrucomicrobia, and Epsilonbacteraeota at NR were strongly positively correlated with total nitrogen (TN), total phosphorus (TP), nitrate nitrogen (NO(3)-N), and dissolved inorganic nitrogen (DIN), but negatively correlated with nitrite nitrogen (NO(2)-N). The relative abundance of Cyanobacteria at NA and LJ were strongly positively correlated with NO(2)-N, but negatively correlated with TN, TP, NO(3)-N, and DIN. Besides, the Mantel test results indicated that the EBC composition was significantly correlated with these environmental factors, which was also confirmed by Spearman correlation analysis. Thus, environmental factors such as NO(3)-N and DIN play a key role in the community composition of epiphytic bacteria on G. lemaneiformis. This study provides important baseline knowledge on the community composition of epiphytic bacteria on G. lemaneiformis and shows correlation between different epiphytic bacteria and their surrounding environmental factors. Nature Publishing Group UK 2021-04-21 /pmc/articles/PMC8060329/ /pubmed/33883606 http://dx.doi.org/10.1038/s41598-021-87977-3 Text en © The Author(s) 2021, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Pei, Pengbing Aslam, Muhammad Du, Hong Liang, Honghao Wang, Hui Liu, Xiaojuan Chen, Weizhou Environmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis |
title | Environmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis |
title_full | Environmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis |
title_fullStr | Environmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis |
title_full_unstemmed | Environmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis |
title_short | Environmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis |
title_sort | environmental factors shape the epiphytic bacterial communities of gracilariopsis lemaneiformis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060329/ https://www.ncbi.nlm.nih.gov/pubmed/33883606 http://dx.doi.org/10.1038/s41598-021-87977-3 |
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