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Expanding plant genome-editing scope by an engineered iSpyMacCas9 system that targets A-rich PAM sequences

The most popular CRISPR-SpCas9 system recognizes canonical NGG protospacer adjacent motifs (PAMs). Previously engineered SpCas9 variants, such as Cas9-NG, favor G-rich PAMs in genome editing. In this manuscript, we describe a new plant genome-editing system based on a hybrid iSpyMacCas9 platform tha...

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Detalles Bibliográficos
Autores principales:	Sretenovic, Simon, Yin, Desuo, Levav, Adam, Selengut, Jeremy D., Mount, Stephen M., Qi, Yiping
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2020
Materias:	Resource Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060698/ https://www.ncbi.nlm.nih.gov/pubmed/33898973 http://dx.doi.org/10.1016/j.xplc.2020.100101

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