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High-efficiency genome editing in plants mediated by a Cas9 gene containing multiple introns
The recent discovery of the mode of action of the CRISPR/Cas9 system has provided biologists with a useful tool for generating site-specific mutations in genes of interest. In plants, site-targeted mutations are usually obtained by the stable transformation of a Cas9 expression construct into the pl...
Autores principales: | Grützner, Ramona, Martin, Patrick, Horn, Claudia, Mortensen, Samuel, Cram, Erin J., Lee-Parsons, Carolyn W.T., Stuttmann, Johannes, Marillonnet, Sylvestre |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060730/ https://www.ncbi.nlm.nih.gov/pubmed/33898975 http://dx.doi.org/10.1016/j.xplc.2020.100135 |
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