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Long non-coding RNA LINC00460 serves as a potential biomarker and oncogene via regulation of the miR-320b/PBX3 axis in acute myeloid leukemia

Long non-coding RNA 00460 (LINC00460) has been reported to be involved in the tumorigenesis of various cancer types. However, the function of LINC00460 in acute myeloid leukemia (AML) remains elusive. Therefore, the present study aimed to investigate the role of LINC00460 in AML. The expression of L...

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Autores principales: Zhuang, Qiang, Jin, Zhenlin, Zheng, Xiangkuo, Jin, Ting, Xiang, Lina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060808/
https://www.ncbi.nlm.nih.gov/pubmed/33846790
http://dx.doi.org/10.3892/mmr.2021.12074
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author Zhuang, Qiang
Jin, Zhenlin
Zheng, Xiangkuo
Jin, Ting
Xiang, Lina
author_facet Zhuang, Qiang
Jin, Zhenlin
Zheng, Xiangkuo
Jin, Ting
Xiang, Lina
author_sort Zhuang, Qiang
collection PubMed
description Long non-coding RNA 00460 (LINC00460) has been reported to be involved in the tumorigenesis of various cancer types. However, the function of LINC00460 in acute myeloid leukemia (AML) remains elusive. Therefore, the present study aimed to investigate the role of LINC00460 in AML. The expression of LINC00460 in the serum of 80 diagnosed patients with AML and 67 healthy controls was measured via reverse transcription-quantitative polymerase chain reaction, and the results were compared with clinical features and patient outcomes. The expression of LINC00460 in 45 patients with cytogenetically normal-AML (CN-AML) was also assayed. Receiver operating characteristic (ROC) curves were generated to evaluate the sensitivity and specificity of serum LINC00460. In addition, the effects of LINC00460 on the viability, cell cycle distribution and apoptosis of AML cells were investigated. Bioinformatics tools were used to identify the possible mechanisms of how LINC00460 affects AML cells. It was found that the expression of LINC00460 was significantly upregulated in the serum of patients with AML and those with CN-AML. Higher expression of serum LINC00460 was positively associated with French-American-British classification and cytogenetics. Furthermore, ROC curve analyses demonstrated that serum LINC00460 could differentiate patients with AML from healthy individuals with an area under the curve of 0.8488 (95% CI, 0.7697–0.9279). The serum LINC00460 expression was also significantly decreased when the patients achieved complete remission. Kaplan-Meier analysis indicated that patients with high serum LINC00460 expression had a shorter overall survival time compared with the low serum LINC00460 expression group. Knockdown of LINC00460 inhibited viability, while inducing cell cycle arrest and apoptosis in AML cells. LINC00460 was also a decoy of microRNA (miR)-320b, which can further inhibit the expression of PBX homeobox 3 (PBX3). Collectively, the results suggested that LINC00460 may be applied as a potential diagnostic and prognostic biomarker for patients with AML. It was identified that LINC00460 may exert its effects, at least partly, via the miR-320b/PBX3 axis in AML.
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spelling pubmed-80608082021-04-25 Long non-coding RNA LINC00460 serves as a potential biomarker and oncogene via regulation of the miR-320b/PBX3 axis in acute myeloid leukemia Zhuang, Qiang Jin, Zhenlin Zheng, Xiangkuo Jin, Ting Xiang, Lina Mol Med Rep Articles Long non-coding RNA 00460 (LINC00460) has been reported to be involved in the tumorigenesis of various cancer types. However, the function of LINC00460 in acute myeloid leukemia (AML) remains elusive. Therefore, the present study aimed to investigate the role of LINC00460 in AML. The expression of LINC00460 in the serum of 80 diagnosed patients with AML and 67 healthy controls was measured via reverse transcription-quantitative polymerase chain reaction, and the results were compared with clinical features and patient outcomes. The expression of LINC00460 in 45 patients with cytogenetically normal-AML (CN-AML) was also assayed. Receiver operating characteristic (ROC) curves were generated to evaluate the sensitivity and specificity of serum LINC00460. In addition, the effects of LINC00460 on the viability, cell cycle distribution and apoptosis of AML cells were investigated. Bioinformatics tools were used to identify the possible mechanisms of how LINC00460 affects AML cells. It was found that the expression of LINC00460 was significantly upregulated in the serum of patients with AML and those with CN-AML. Higher expression of serum LINC00460 was positively associated with French-American-British classification and cytogenetics. Furthermore, ROC curve analyses demonstrated that serum LINC00460 could differentiate patients with AML from healthy individuals with an area under the curve of 0.8488 (95% CI, 0.7697–0.9279). The serum LINC00460 expression was also significantly decreased when the patients achieved complete remission. Kaplan-Meier analysis indicated that patients with high serum LINC00460 expression had a shorter overall survival time compared with the low serum LINC00460 expression group. Knockdown of LINC00460 inhibited viability, while inducing cell cycle arrest and apoptosis in AML cells. LINC00460 was also a decoy of microRNA (miR)-320b, which can further inhibit the expression of PBX homeobox 3 (PBX3). Collectively, the results suggested that LINC00460 may be applied as a potential diagnostic and prognostic biomarker for patients with AML. It was identified that LINC00460 may exert its effects, at least partly, via the miR-320b/PBX3 axis in AML. D.A. Spandidos 2021-06 2021-04-08 /pmc/articles/PMC8060808/ /pubmed/33846790 http://dx.doi.org/10.3892/mmr.2021.12074 Text en Copyright: © Zhuang et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhuang, Qiang
Jin, Zhenlin
Zheng, Xiangkuo
Jin, Ting
Xiang, Lina
Long non-coding RNA LINC00460 serves as a potential biomarker and oncogene via regulation of the miR-320b/PBX3 axis in acute myeloid leukemia
title Long non-coding RNA LINC00460 serves as a potential biomarker and oncogene via regulation of the miR-320b/PBX3 axis in acute myeloid leukemia
title_full Long non-coding RNA LINC00460 serves as a potential biomarker and oncogene via regulation of the miR-320b/PBX3 axis in acute myeloid leukemia
title_fullStr Long non-coding RNA LINC00460 serves as a potential biomarker and oncogene via regulation of the miR-320b/PBX3 axis in acute myeloid leukemia
title_full_unstemmed Long non-coding RNA LINC00460 serves as a potential biomarker and oncogene via regulation of the miR-320b/PBX3 axis in acute myeloid leukemia
title_short Long non-coding RNA LINC00460 serves as a potential biomarker and oncogene via regulation of the miR-320b/PBX3 axis in acute myeloid leukemia
title_sort long non-coding rna linc00460 serves as a potential biomarker and oncogene via regulation of the mir-320b/pbx3 axis in acute myeloid leukemia
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060808/
https://www.ncbi.nlm.nih.gov/pubmed/33846790
http://dx.doi.org/10.3892/mmr.2021.12074
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