Cargando…

Chiasmata and the kinetochore component Dam1 are crucial for elimination of erroneous chromosome attachments and centromere oscillation at meiosis I

Establishment of proper chromosome attachments to the spindle requires elimination of erroneous attachments, but the mechanism of this process is not fully understood. During meiosis I, sister chromatids attach to the same spindle pole (mono-oriented attachment), whereas homologous chromosomes attac...

Descripción completa

Detalles Bibliográficos
Autores principales: Wakiya, Misuzu, Nishi, Eriko, Kawai, Shinnosuke, Yamada, Kohei, Katsumata, Kazuhiro, Hirayasu, Ami, Itabashi, Yuta, Yamamoto, Ayumu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061696/
https://www.ncbi.nlm.nih.gov/pubmed/33529549
http://dx.doi.org/10.1098/rsob.200308
_version_ 1783681620106018816
author Wakiya, Misuzu
Nishi, Eriko
Kawai, Shinnosuke
Yamada, Kohei
Katsumata, Kazuhiro
Hirayasu, Ami
Itabashi, Yuta
Yamamoto, Ayumu
author_facet Wakiya, Misuzu
Nishi, Eriko
Kawai, Shinnosuke
Yamada, Kohei
Katsumata, Kazuhiro
Hirayasu, Ami
Itabashi, Yuta
Yamamoto, Ayumu
author_sort Wakiya, Misuzu
collection PubMed
description Establishment of proper chromosome attachments to the spindle requires elimination of erroneous attachments, but the mechanism of this process is not fully understood. During meiosis I, sister chromatids attach to the same spindle pole (mono-oriented attachment), whereas homologous chromosomes attach to opposite poles (bi-oriented attachment), resulting in homologous chromosome segregation. Here, we show that chiasmata that link homologous chromosomes and kinetochore component Dam1 are crucial for elimination of erroneous attachments and oscillation of centromeres between the spindle poles at meiosis I in fission yeast. In chiasma-forming cells, Mad2 and Aurora B kinase, which provides time for attachment correction and destabilizes erroneous attachments, respectively, caused elimination of bi-oriented attachments of sister chromatids, whereas in chiasma-lacking cells, they caused elimination of mono-oriented attachments. In chiasma-forming cells, in addition, homologous centromere oscillation was coordinated. Furthermore, Dam1 contributed to attachment elimination in both chiasma-forming and chiasma-lacking cells, and drove centromere oscillation. These results demonstrate that chiasmata alter attachment correction patterns by enabling error correction factors to eliminate bi-oriented attachment of sister chromatids, and suggest that Dam1 induces elimination of erroneous attachments. The coincidental contribution of chiasmata and Dam1 to centromere oscillation also suggests a potential link between centromere oscillation and attachment elimination.
format Online
Article
Text
id pubmed-8061696
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher The Royal Society
record_format MEDLINE/PubMed
spelling pubmed-80616962021-05-14 Chiasmata and the kinetochore component Dam1 are crucial for elimination of erroneous chromosome attachments and centromere oscillation at meiosis I Wakiya, Misuzu Nishi, Eriko Kawai, Shinnosuke Yamada, Kohei Katsumata, Kazuhiro Hirayasu, Ami Itabashi, Yuta Yamamoto, Ayumu Open Biol Research Establishment of proper chromosome attachments to the spindle requires elimination of erroneous attachments, but the mechanism of this process is not fully understood. During meiosis I, sister chromatids attach to the same spindle pole (mono-oriented attachment), whereas homologous chromosomes attach to opposite poles (bi-oriented attachment), resulting in homologous chromosome segregation. Here, we show that chiasmata that link homologous chromosomes and kinetochore component Dam1 are crucial for elimination of erroneous attachments and oscillation of centromeres between the spindle poles at meiosis I in fission yeast. In chiasma-forming cells, Mad2 and Aurora B kinase, which provides time for attachment correction and destabilizes erroneous attachments, respectively, caused elimination of bi-oriented attachments of sister chromatids, whereas in chiasma-lacking cells, they caused elimination of mono-oriented attachments. In chiasma-forming cells, in addition, homologous centromere oscillation was coordinated. Furthermore, Dam1 contributed to attachment elimination in both chiasma-forming and chiasma-lacking cells, and drove centromere oscillation. These results demonstrate that chiasmata alter attachment correction patterns by enabling error correction factors to eliminate bi-oriented attachment of sister chromatids, and suggest that Dam1 induces elimination of erroneous attachments. The coincidental contribution of chiasmata and Dam1 to centromere oscillation also suggests a potential link between centromere oscillation and attachment elimination. The Royal Society 2021-02-03 /pmc/articles/PMC8061696/ /pubmed/33529549 http://dx.doi.org/10.1098/rsob.200308 Text en © 2021 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited.
spellingShingle Research
Wakiya, Misuzu
Nishi, Eriko
Kawai, Shinnosuke
Yamada, Kohei
Katsumata, Kazuhiro
Hirayasu, Ami
Itabashi, Yuta
Yamamoto, Ayumu
Chiasmata and the kinetochore component Dam1 are crucial for elimination of erroneous chromosome attachments and centromere oscillation at meiosis I
title Chiasmata and the kinetochore component Dam1 are crucial for elimination of erroneous chromosome attachments and centromere oscillation at meiosis I
title_full Chiasmata and the kinetochore component Dam1 are crucial for elimination of erroneous chromosome attachments and centromere oscillation at meiosis I
title_fullStr Chiasmata and the kinetochore component Dam1 are crucial for elimination of erroneous chromosome attachments and centromere oscillation at meiosis I
title_full_unstemmed Chiasmata and the kinetochore component Dam1 are crucial for elimination of erroneous chromosome attachments and centromere oscillation at meiosis I
title_short Chiasmata and the kinetochore component Dam1 are crucial for elimination of erroneous chromosome attachments and centromere oscillation at meiosis I
title_sort chiasmata and the kinetochore component dam1 are crucial for elimination of erroneous chromosome attachments and centromere oscillation at meiosis i
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061696/
https://www.ncbi.nlm.nih.gov/pubmed/33529549
http://dx.doi.org/10.1098/rsob.200308
work_keys_str_mv AT wakiyamisuzu chiasmataandthekinetochorecomponentdam1arecrucialforeliminationoferroneouschromosomeattachmentsandcentromereoscillationatmeiosisi
AT nishieriko chiasmataandthekinetochorecomponentdam1arecrucialforeliminationoferroneouschromosomeattachmentsandcentromereoscillationatmeiosisi
AT kawaishinnosuke chiasmataandthekinetochorecomponentdam1arecrucialforeliminationoferroneouschromosomeattachmentsandcentromereoscillationatmeiosisi
AT yamadakohei chiasmataandthekinetochorecomponentdam1arecrucialforeliminationoferroneouschromosomeattachmentsandcentromereoscillationatmeiosisi
AT katsumatakazuhiro chiasmataandthekinetochorecomponentdam1arecrucialforeliminationoferroneouschromosomeattachmentsandcentromereoscillationatmeiosisi
AT hirayasuami chiasmataandthekinetochorecomponentdam1arecrucialforeliminationoferroneouschromosomeattachmentsandcentromereoscillationatmeiosisi
AT itabashiyuta chiasmataandthekinetochorecomponentdam1arecrucialforeliminationoferroneouschromosomeattachmentsandcentromereoscillationatmeiosisi
AT yamamotoayumu chiasmataandthekinetochorecomponentdam1arecrucialforeliminationoferroneouschromosomeattachmentsandcentromereoscillationatmeiosisi