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A two-step PCR assembly for construction of gene variants across large mutational distances
Construction of empirical fitness landscapes has transformed our understanding of genotype–phenotype relationships across genes. However, most empirical fitness landscapes have been constrained to the local genotype neighbourhood of a gene primarily due to our limited ability to systematically const...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062255/ https://www.ncbi.nlm.nih.gov/pubmed/33928191 http://dx.doi.org/10.1093/biomethods/bpab007 |