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Microbial Key Players Involved in P Turnover Differ in Artificial Soil Mixtures Depending on Clay Mineral Composition
Nutrient turnover in soils is strongly driven by soil properties, including clay mineral composition. One main nutrient is phosphorus (P), which is known to be easily immobilized in soil. Therefore, the specific surface characteristics of clay minerals might substantially influence P availability in...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062357/ https://www.ncbi.nlm.nih.gov/pubmed/33161521 http://dx.doi.org/10.1007/s00248-020-01635-1 |
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author | Tanuwidjaja, Irina Vogel, Cordula Pronk, Geertje J. Schöler, Anne Kublik, Susanne Vestergaard, Gisle Kögel-Knabner, Ingrid Mrkonjic Fuka, Mirna Schloter, Michael Schulz, Stefanie |
author_facet | Tanuwidjaja, Irina Vogel, Cordula Pronk, Geertje J. Schöler, Anne Kublik, Susanne Vestergaard, Gisle Kögel-Knabner, Ingrid Mrkonjic Fuka, Mirna Schloter, Michael Schulz, Stefanie |
author_sort | Tanuwidjaja, Irina |
collection | PubMed |
description | Nutrient turnover in soils is strongly driven by soil properties, including clay mineral composition. One main nutrient is phosphorus (P), which is known to be easily immobilized in soil. Therefore, the specific surface characteristics of clay minerals might substantially influence P availability in soil and thus the microbial strategies for accessing P pools. We used a metagenomic approach to analyze the microbial potential to access P after 842 days of incubation in artificial soils with a clay mineral composition of either non-expandable illite (IL) or expandable montmorillonite (MT), which differ in their surface characteristics like soil surface area and surface charge. Our data indicate that microorganisms of the two soils developed different strategies to overcome P depletion, resulting in similar total P concentrations. Genes predicted to encode inorganic pyrophosphatase (ppa), exopolyphosphatase (ppx), and the pstSCAB transport system were higher in MT, suggesting effective P uptake and the use of internal poly-P stores. Genes predicted to encode enzymes involved in organic P turnover like alkaline phosphatases (phoA, phoD) and glycerophosphoryl diester phosphodiesterase were detected in both soils in comparable numbers. In addition, P(o) concentrations did not differ significantly. Most identified genes were assigned to microbial lineages generally abundant in agricultural fields, but some were assigned to lineages known to include oligotrophic specialists, such as Bacillaceae and Microchaetaceae. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00248-020-01635-1. |
format | Online Article Text |
id | pubmed-8062357 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-80623572021-05-05 Microbial Key Players Involved in P Turnover Differ in Artificial Soil Mixtures Depending on Clay Mineral Composition Tanuwidjaja, Irina Vogel, Cordula Pronk, Geertje J. Schöler, Anne Kublik, Susanne Vestergaard, Gisle Kögel-Knabner, Ingrid Mrkonjic Fuka, Mirna Schloter, Michael Schulz, Stefanie Microb Ecol Environmental Microbiology Nutrient turnover in soils is strongly driven by soil properties, including clay mineral composition. One main nutrient is phosphorus (P), which is known to be easily immobilized in soil. Therefore, the specific surface characteristics of clay minerals might substantially influence P availability in soil and thus the microbial strategies for accessing P pools. We used a metagenomic approach to analyze the microbial potential to access P after 842 days of incubation in artificial soils with a clay mineral composition of either non-expandable illite (IL) or expandable montmorillonite (MT), which differ in their surface characteristics like soil surface area and surface charge. Our data indicate that microorganisms of the two soils developed different strategies to overcome P depletion, resulting in similar total P concentrations. Genes predicted to encode inorganic pyrophosphatase (ppa), exopolyphosphatase (ppx), and the pstSCAB transport system were higher in MT, suggesting effective P uptake and the use of internal poly-P stores. Genes predicted to encode enzymes involved in organic P turnover like alkaline phosphatases (phoA, phoD) and glycerophosphoryl diester phosphodiesterase were detected in both soils in comparable numbers. In addition, P(o) concentrations did not differ significantly. Most identified genes were assigned to microbial lineages generally abundant in agricultural fields, but some were assigned to lineages known to include oligotrophic specialists, such as Bacillaceae and Microchaetaceae. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00248-020-01635-1. Springer US 2020-11-07 2021 /pmc/articles/PMC8062357/ /pubmed/33161521 http://dx.doi.org/10.1007/s00248-020-01635-1 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Environmental Microbiology Tanuwidjaja, Irina Vogel, Cordula Pronk, Geertje J. Schöler, Anne Kublik, Susanne Vestergaard, Gisle Kögel-Knabner, Ingrid Mrkonjic Fuka, Mirna Schloter, Michael Schulz, Stefanie Microbial Key Players Involved in P Turnover Differ in Artificial Soil Mixtures Depending on Clay Mineral Composition |
title | Microbial Key Players Involved in P Turnover Differ in Artificial Soil Mixtures Depending on Clay Mineral Composition |
title_full | Microbial Key Players Involved in P Turnover Differ in Artificial Soil Mixtures Depending on Clay Mineral Composition |
title_fullStr | Microbial Key Players Involved in P Turnover Differ in Artificial Soil Mixtures Depending on Clay Mineral Composition |
title_full_unstemmed | Microbial Key Players Involved in P Turnover Differ in Artificial Soil Mixtures Depending on Clay Mineral Composition |
title_short | Microbial Key Players Involved in P Turnover Differ in Artificial Soil Mixtures Depending on Clay Mineral Composition |
title_sort | microbial key players involved in p turnover differ in artificial soil mixtures depending on clay mineral composition |
topic | Environmental Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062357/ https://www.ncbi.nlm.nih.gov/pubmed/33161521 http://dx.doi.org/10.1007/s00248-020-01635-1 |
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