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Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae

In our previous study, fatty acid-binding protein 5 (FABP5) was expressed in septoclasts with long processes which are considered to resorb uncalcified matrix of the growth plate (GP) cartilage, and no apparent abnormalities were detected in the histo-architecture of the GP of FABP5-deficient (FABP5...

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Detalles Bibliográficos
Autores principales: Bando, Yasuhiko, Tokuda, Nobuko, Ogasawara, Yudai, Onozawa, Go, Nagasaka, Arata, Sakiyama, Koji, Owada, Yuji, Amano, Osamu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062382/
https://www.ncbi.nlm.nih.gov/pubmed/33398436
http://dx.doi.org/10.1007/s00418-020-01953-y
Descripción
Sumario:In our previous study, fatty acid-binding protein 5 (FABP5) was expressed in septoclasts with long processes which are considered to resorb uncalcified matrix of the growth plate (GP) cartilage, and no apparent abnormalities were detected in the histo-architecture of the GP of FABP5-deficient (FABP5(−/−)) mice. Those finding lead us to hypothesize that another FABP can compensate the deletion of FABP5 in septoclasts of its gene-mutant mice. Based on the hypothesis, the present study examined the expression levels of several other FABPs in septoclasts and their morphology in FABP5(−/−) mouse tibiae. Processes of FABP5(−/−) septoclasts tend to be shorter than wild septoclasts. FABP4-positive septoclasts in FABP5(−/−) mice were more numerous than those cells in wild mice. Peroxisome proliferator-activated receptor (PPAR) γ was expressed in FABP4-positive septoclasts of FABP5(−/−) mice as well as mice administered with GW1929, a PPARγ agonist, suggesting that the occurrence of PPARγ induces an increase of FABP4-positive septoclasts. The present finding suggests that the functional exertion of FABP5 in septoclasts is supplemented by FABP4 in normal and FABP5(−/−) mice, and that the expression of FABP4 is up-regulated in accompany with PPARγ in FABP5(−/−) for maintenance of resorptive activity in the GP. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00418-020-01953-y.