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Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae

In our previous study, fatty acid-binding protein 5 (FABP5) was expressed in septoclasts with long processes which are considered to resorb uncalcified matrix of the growth plate (GP) cartilage, and no apparent abnormalities were detected in the histo-architecture of the GP of FABP5-deficient (FABP5...

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Autores principales: Bando, Yasuhiko, Tokuda, Nobuko, Ogasawara, Yudai, Onozawa, Go, Nagasaka, Arata, Sakiyama, Koji, Owada, Yuji, Amano, Osamu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062382/
https://www.ncbi.nlm.nih.gov/pubmed/33398436
http://dx.doi.org/10.1007/s00418-020-01953-y
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author Bando, Yasuhiko
Tokuda, Nobuko
Ogasawara, Yudai
Onozawa, Go
Nagasaka, Arata
Sakiyama, Koji
Owada, Yuji
Amano, Osamu
author_facet Bando, Yasuhiko
Tokuda, Nobuko
Ogasawara, Yudai
Onozawa, Go
Nagasaka, Arata
Sakiyama, Koji
Owada, Yuji
Amano, Osamu
author_sort Bando, Yasuhiko
collection PubMed
description In our previous study, fatty acid-binding protein 5 (FABP5) was expressed in septoclasts with long processes which are considered to resorb uncalcified matrix of the growth plate (GP) cartilage, and no apparent abnormalities were detected in the histo-architecture of the GP of FABP5-deficient (FABP5(−/−)) mice. Those finding lead us to hypothesize that another FABP can compensate the deletion of FABP5 in septoclasts of its gene-mutant mice. Based on the hypothesis, the present study examined the expression levels of several other FABPs in septoclasts and their morphology in FABP5(−/−) mouse tibiae. Processes of FABP5(−/−) septoclasts tend to be shorter than wild septoclasts. FABP4-positive septoclasts in FABP5(−/−) mice were more numerous than those cells in wild mice. Peroxisome proliferator-activated receptor (PPAR) γ was expressed in FABP4-positive septoclasts of FABP5(−/−) mice as well as mice administered with GW1929, a PPARγ agonist, suggesting that the occurrence of PPARγ induces an increase of FABP4-positive septoclasts. The present finding suggests that the functional exertion of FABP5 in septoclasts is supplemented by FABP4 in normal and FABP5(−/−) mice, and that the expression of FABP4 is up-regulated in accompany with PPARγ in FABP5(−/−) for maintenance of resorptive activity in the GP. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00418-020-01953-y.
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spelling pubmed-80623822021-05-05 Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae Bando, Yasuhiko Tokuda, Nobuko Ogasawara, Yudai Onozawa, Go Nagasaka, Arata Sakiyama, Koji Owada, Yuji Amano, Osamu Histochem Cell Biol Original Paper In our previous study, fatty acid-binding protein 5 (FABP5) was expressed in septoclasts with long processes which are considered to resorb uncalcified matrix of the growth plate (GP) cartilage, and no apparent abnormalities were detected in the histo-architecture of the GP of FABP5-deficient (FABP5(−/−)) mice. Those finding lead us to hypothesize that another FABP can compensate the deletion of FABP5 in septoclasts of its gene-mutant mice. Based on the hypothesis, the present study examined the expression levels of several other FABPs in septoclasts and their morphology in FABP5(−/−) mouse tibiae. Processes of FABP5(−/−) septoclasts tend to be shorter than wild septoclasts. FABP4-positive septoclasts in FABP5(−/−) mice were more numerous than those cells in wild mice. Peroxisome proliferator-activated receptor (PPAR) γ was expressed in FABP4-positive septoclasts of FABP5(−/−) mice as well as mice administered with GW1929, a PPARγ agonist, suggesting that the occurrence of PPARγ induces an increase of FABP4-positive septoclasts. The present finding suggests that the functional exertion of FABP5 in septoclasts is supplemented by FABP4 in normal and FABP5(−/−) mice, and that the expression of FABP4 is up-regulated in accompany with PPARγ in FABP5(−/−) for maintenance of resorptive activity in the GP. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00418-020-01953-y. Springer Berlin Heidelberg 2021-01-04 2021 /pmc/articles/PMC8062382/ /pubmed/33398436 http://dx.doi.org/10.1007/s00418-020-01953-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Paper
Bando, Yasuhiko
Tokuda, Nobuko
Ogasawara, Yudai
Onozawa, Go
Nagasaka, Arata
Sakiyama, Koji
Owada, Yuji
Amano, Osamu
Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae
title Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae
title_full Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae
title_fullStr Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae
title_full_unstemmed Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae
title_short Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae
title_sort expression and enhancement of fabp4 in septoclasts of the growth plate in fabp5-deficient mouse tibiae
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062382/
https://www.ncbi.nlm.nih.gov/pubmed/33398436
http://dx.doi.org/10.1007/s00418-020-01953-y
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