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Lepidopteran wing scales contain abundant cross-linked film-forming histidine-rich cuticular proteins
Scales are symbolic characteristic of Lepidoptera; however, nothing is known about the contribution of cuticular proteins (CPs) to the complex patterning of lepidopteran scales. This is because scales are resistant to solubilization, thus hindering molecular studies. Here we succeeded in dissolving...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062583/ https://www.ncbi.nlm.nih.gov/pubmed/33888855 http://dx.doi.org/10.1038/s42003-021-01996-4 |
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author | Liu, Jianqiu Chen, Zhiwei Xiao, Yingdan Asano, Tsunaki Li, Shenglong Peng, Li Chen, Enxiang Zhang, Jiwei Li, Wanshun Zhang, Yan Tong, Xiaoling Kadono-Okuda, Keiko Zhao, Ping He, Ningjia Arunkumar, Kallare P. Gopinathan, Karumathil P. Xia, Qingyou Willis, Judith H. Goldsmith, Marian R. Mita, Kazuei |
author_facet | Liu, Jianqiu Chen, Zhiwei Xiao, Yingdan Asano, Tsunaki Li, Shenglong Peng, Li Chen, Enxiang Zhang, Jiwei Li, Wanshun Zhang, Yan Tong, Xiaoling Kadono-Okuda, Keiko Zhao, Ping He, Ningjia Arunkumar, Kallare P. Gopinathan, Karumathil P. Xia, Qingyou Willis, Judith H. Goldsmith, Marian R. Mita, Kazuei |
author_sort | Liu, Jianqiu |
collection | PubMed |
description | Scales are symbolic characteristic of Lepidoptera; however, nothing is known about the contribution of cuticular proteins (CPs) to the complex patterning of lepidopteran scales. This is because scales are resistant to solubilization, thus hindering molecular studies. Here we succeeded in dissolving developing wing scales from Bombyx mori, allowing analysis of their protein composition. We identified a distinctive class of histidine rich (His-rich) CPs (6%–45%) from developing lepidopteran scales by LC-MS/MS. Functional studies using RNAi revealed CPs with different histidine content play distinct and critical roles in constructing the microstructure of the scale surface. Moreover, we successfully synthesized films in vitro by crosslinking a 45% His-rich CP (BmorCPR152) with laccase2 using N-acetyl- dopamine or N-β-alanyl-dopamine as the substrate. This molecular study of scales provides fundamental information about how such a fine microstructure is constructed and insights into the potential application of CPs as new biomaterials. |
format | Online Article Text |
id | pubmed-8062583 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-80625832021-05-05 Lepidopteran wing scales contain abundant cross-linked film-forming histidine-rich cuticular proteins Liu, Jianqiu Chen, Zhiwei Xiao, Yingdan Asano, Tsunaki Li, Shenglong Peng, Li Chen, Enxiang Zhang, Jiwei Li, Wanshun Zhang, Yan Tong, Xiaoling Kadono-Okuda, Keiko Zhao, Ping He, Ningjia Arunkumar, Kallare P. Gopinathan, Karumathil P. Xia, Qingyou Willis, Judith H. Goldsmith, Marian R. Mita, Kazuei Commun Biol Article Scales are symbolic characteristic of Lepidoptera; however, nothing is known about the contribution of cuticular proteins (CPs) to the complex patterning of lepidopteran scales. This is because scales are resistant to solubilization, thus hindering molecular studies. Here we succeeded in dissolving developing wing scales from Bombyx mori, allowing analysis of their protein composition. We identified a distinctive class of histidine rich (His-rich) CPs (6%–45%) from developing lepidopteran scales by LC-MS/MS. Functional studies using RNAi revealed CPs with different histidine content play distinct and critical roles in constructing the microstructure of the scale surface. Moreover, we successfully synthesized films in vitro by crosslinking a 45% His-rich CP (BmorCPR152) with laccase2 using N-acetyl- dopamine or N-β-alanyl-dopamine as the substrate. This molecular study of scales provides fundamental information about how such a fine microstructure is constructed and insights into the potential application of CPs as new biomaterials. Nature Publishing Group UK 2021-04-22 /pmc/articles/PMC8062583/ /pubmed/33888855 http://dx.doi.org/10.1038/s42003-021-01996-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Liu, Jianqiu Chen, Zhiwei Xiao, Yingdan Asano, Tsunaki Li, Shenglong Peng, Li Chen, Enxiang Zhang, Jiwei Li, Wanshun Zhang, Yan Tong, Xiaoling Kadono-Okuda, Keiko Zhao, Ping He, Ningjia Arunkumar, Kallare P. Gopinathan, Karumathil P. Xia, Qingyou Willis, Judith H. Goldsmith, Marian R. Mita, Kazuei Lepidopteran wing scales contain abundant cross-linked film-forming histidine-rich cuticular proteins |
title | Lepidopteran wing scales contain abundant cross-linked film-forming histidine-rich cuticular proteins |
title_full | Lepidopteran wing scales contain abundant cross-linked film-forming histidine-rich cuticular proteins |
title_fullStr | Lepidopteran wing scales contain abundant cross-linked film-forming histidine-rich cuticular proteins |
title_full_unstemmed | Lepidopteran wing scales contain abundant cross-linked film-forming histidine-rich cuticular proteins |
title_short | Lepidopteran wing scales contain abundant cross-linked film-forming histidine-rich cuticular proteins |
title_sort | lepidopteran wing scales contain abundant cross-linked film-forming histidine-rich cuticular proteins |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062583/ https://www.ncbi.nlm.nih.gov/pubmed/33888855 http://dx.doi.org/10.1038/s42003-021-01996-4 |
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