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Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles

Cell-free gene expression (CFE) systems from crude cellular extracts have attracted much attention for biomanufacturing and synthetic biology. However, activating membrane-dependent functionality of cell-derived vesicles in bacterial CFE systems has been limited. Here, we address this limitation by...

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Detalles Bibliográficos
Autores principales:	Hershewe, Jasmine M., Warfel, Katherine F., Iyer, Shaelyn M., Peruzzi, Justin A., Sullivan, Claretta J., Roth, Eric W., DeLisa, Matthew P., Kamat, Neha P., Jewett, Michael C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062659/ https://www.ncbi.nlm.nih.gov/pubmed/33888690 http://dx.doi.org/10.1038/s41467-021-22329-3

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